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| 1 | Production of transgenic calves by somatic cell nuclear transfer显示文摘Bovine fetal oviduct epithelial cells were transfected with constructed double marker selective vector(pCE-EGFP-IRES-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant(Neo^r) genes by electroporation, and a transgenic cell line was obtained. Somatic cell nuclear transfer (SCNT) was cartied out using the transgenic cells as nuclei donor. A total of 424 SCNT embryos were reconstructed and 208 (49.1%) of them developed to blastocyst stage. 17 blastocysts on D 7 after reconstruction were transferred to 17 surrogate calves,and 5 (29.4%) recipients were found to be pregnant. Three of them maintained to term and delivered three cloned calves.PCR and Southern blot analysis confirmed the integration of transgene in all of the three cloned calves. In addition, expression of EGFP was detected in biopsy isolated from the transgenic cloned calves and fibroblasts derived from the biopsy. Our results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor. Furthermore, all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic cells and SCNT embryos using the constructed double marker selective vector. | GONGGuochun DAIYunping FANBaoliang ZHUHuabing WANGLili WANGHaiping TANGBo LIUYing LIRong WANGRong HUANGYinghua LINing | 2004 | Chinese Science Bulletin2004，49，2： | 7 |
| 2 | Preparation of magnetic ordered mesoporous carbon composit and its application in direct electrochemistry of horseradish peroxidase显示文摘 | ZHENG Jing XU Jingli JIN Tangbo | 2013 | Electroanalysis2013，25，9： | 1 |
| 3 | Ab Initio Calculation of Room Temperature Ionic Liquid 1-Ethyl-3-Methyl-Imidazolium Chlorocuprate (Ⅰ)显示文摘Hartree-Fock 被采用了调查 EMIM (+)(1-ethyl-3-methyl-imidazolium (+)) 的电子结构, CuCl (?) 2, Cu2Cl (?) 3, CuCl2 (?) , EMIM (+)-CuCl(?)2, EMIM (+)-Cu2Cl(?)3, 和 EMIM (+)-CuCl(2?)3 配对。 EMIM (+)的完整的优化和频率分析, CuCl (?) 2 , Cu2Cl (?) 3 , CuCl ( 2 ?) 3 ,八起始的 EMIM (+)-CuCl(?)2,六 EMIM (+)-Cu2Cl(?)3,和四 EMIM (+) -CuCl(2?)3 几何学用在 6-31+G 软包裹的 Gaussian-94 被执行了(氘的符号, p )为氢,碳,氮,氯原子和 Hay-Wadt 的基础集合水平为铜的有效核心潜力原子。EMIM (+)-CuCl(?)2, EMIM (+)-Cu2Cl(?)3, EMIM (+)-CuCl(2?)3, 单身者 EMIM (+) 的最低精力的电子结构, CuCl (?) 2, Cu2Cl (?) 3,并且 CuCl (2 ) 3 (?) 比较地被学习了。计算结果证明最低精力的 EMIM (+)-CuCl(?)3 对 conformer 是到 17 号元素氯的化学符号的五戒指平行 ? aCu ? 有 3.2 的 aCl 吗?距离,最低精力的 EMIM (+)-CuCl(2?)3 对 conformer 是到 CuCl (2 ) 的五戒指平行有 3.4 的 3 (?) 飞机?距离,和最低精力的优化 EMIM (+)-Cu2Cl(?)3 对 conformer 是对 17 号元素氯的化学符号垂直的五枚戒指 ? aCu ? aCl ? aCu ? 有 3.0 的 aCl 飞机吗?在终端 17 号元素氯的化学符号原子和 EMIM (+) 的 5 戒指之间的距离。在阳离子和阴离子之间的结合被 6 号元素碳的化学符号引起 ? 啊 ?- 被费用帮助增强的 17 号元素氯的化学符号氢键。频率分析建议因为没有想象的频率出现,所有静止的点是最小的。分配频率与试验性的报告一致。因为 EMIM (+) 和阳离子和阴离子的费用分散的笨重不对称现象,相互作用的低精力导致离子的液体, EMIM (+)-CuCl(?)2, EMIM (+)-Cu2Cl(?)3, 和 EMIM (+)-CuCl(2?)3 的低熔点。EMIM (+)-CuCl(?)2, EMIM (+)-Cu2Cl(?)3, 和 EMIM (+)-CuCl(2?)3 的相互作用精力分别地是 309.0 kJ/mol, 316.8 kJ/mol,和 320.2 kJ/mol。经由在阳离子和阴离子之间的距离的相互作用精力的关系被单个点精力扫描也调查。给词调音:离子的液体;Hartree-Fock;1-ethyl-3-methyl-imidazolium;chlorocuprate (I) | Renqing Lue Hejin Tangbo Zuogang Cao | 2007 | Journal of Natural Gas Chemistry2007，16，1： | 1 |
| 4 | Synthesis of Dimethyl Carbonate from Methanol and Carbon dioxide using Potassium Methoxide as Catalyst under Mild Conditions显示文摘 | Qinghai Cai Chao Jin Bin Lu Hejin Tangbo Yongkui Shan | 2005 | Catalysis Letters2005，，3： | 1 |
| 5 | Complex Model Simplification Algorithm with Improved Error Evaluation显示文摘Given the framework of incremental mesh simplification based on edge collapse, the paper proposes a mesh simplification algorithm using an improved approach for measuring simplification error. The algorithm uses edge collapse to simplify the triangle mesh and maintains surface error approximations using c\|error for the faces which have changed after edge collapse and d\|error for the faces which become degenerated after edge collapse. Also, we report some results using a variety of computer graphics models, which can show that the algorithm can achieve the desired simplification effect. | JIANGHong-bo LUYan-sheng TANGBo | 2005 | Wuhan University Journal of Natural Sciences2005，10，2： | 0 |
| 6 | Sensitized effect of β-cyclodextrin on the fluorescence in the determination of carbaryl显示文摘Based on the significant enhancement of fluorescence intensity of carbaryl in inclusion complex, a spetrofluorimetric method with high sensitivity was developed for the determination of carbaryl in aqueous solution. Under the optimum conditions, the complex had excitation and emission maxima at 278 nm and 332 nm, respectively. The linear range of the method was 7.0 ng/ml—1500 ng/ml with a detection limit of 1.2 ng/ml. The proposed method was successfully used to determine quantitatively of carbaryl in cottonseeds. | LIUWen-li TANGBo | 2004 | Journal of Environmental Sciences2004，16，5： | 0 |
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