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| 1 | Presence of Antibodies to Heat Stress Proteins and its Possible Significance in Workers Exposed to High Temperature and Carbon Monoxide显示文摘Antibodies to the ubiquitous group of stress proteins known as heat shock proteins (Hsps) have been found to be associated with a number of diseases in humans. Hsps are known to be induced by certain xenobiotics, some of which are common in the working environment. The biological significance of the presence of such autoantibodies is presently unclear. In the present study, we used immunoblotting to investigate the presence of antibodies against the different stress proteins, Hsp27, Hsp60, Hsp71, Hse (heat shock cognate ) 73 and Hsp89a and D in groups of workers exposed to high temperature or carbon monoxide. These data were related to a detailed clinical evaluation and to various laboratory measurements including electrocardiogram (ECG), B echogram, white blood cell counts and typing, the activity of alanine aminotransferase (ALT), acid phosphatase (ACP) and alkaline phosphatase (ALP) and lymphocyte DNA damage. Antibodies to Hsp27 and Hsp71 were found more frequently in the high temperature and carbon monoxide-exposed groups than in controls (P (0.05 ). The carbon monoxide-exposed group showed the highest incidence of anti-Hsp antibodies. Anti-Hsp60 antibodies were only detected in workers exposed to high temperature or carbon monoxide. The percentage of workers with abnormal ECG, B echogram changes and displaying hepatitis B antigen (HBsAg ) was higher in the carbon monoxide group than in the control group (P<0.05 ).There was a significant inerease in the activity of ALT in the high temperature and carbon monoxide groups and in the activities of ACP and ALP in the carbon monoxide group (P<0.05 ). The extent of DNA damage measured in lymphoeytes was higher in workers from the high temperature and carbon monoxide-cxposed groups. We suggest that the increased frequeney of antibodies to Hsps is the result of these damages, of the release of denatured Hsps and of a decrease in the phagocytic ability of macrophages in these workers. The data gathered in the present study show a statistical relation between the occurrence of antibodies against Hsps and the frequency of health problems in workers and suggest a potential role for the antibodies as useful biomarkers to assess whether workers are experieneing environmental | WU TANG-CHUN TANGUAY, ROBERT M. WU YANG HE HAN-ZHEN XU DAI-GEN FENG JIA-DE SHI WU-XIANG AND ZHANG GUO-GAO (Institute of Occupational Medicine, Tongji Medical University, Wuhan 430040, China Centre de recherche du CHUL and Lab of Cellular and Develo | 1996 | Biomedical and Environmental Sciences1996，9，4： | 23 |
| 2 | 接触高温、CO工人血浆中的HSPs抗体水平显示文摘应用WesternBlot方法,利用已纯化的HSPs抗原,检测了接触高温、CO工人血浆中HSP27、HSP60、HSP70、HSC70、HSP89α和HSP89β抗体水平。结果发现:工人血浆中HSP27、HSP70抗体阳性检出率以有CO接触职业史者最高,有高温接触史者次之,无两者接触者最低;HSP60抗体仅在有CO、高温接触史人群中出现;HSC70在3组间无差异;HSP89α、HSP89β在3组人群中均未检出。HSPs抗体阳性或阴性者与吸烟、饮酒和既往病史等方面无明显关系,但与职业接触史有关。 | 邬堂春 吴扬 冯家德 徐代根 贺涵贞 张国高 Robert Tanguay | 1995 | 中华劳动卫生职业病杂志1995，13，1： | 15 |
| 3 | PCBs和PBDEs对人类癌细胞和斑马鱼胚胎的毒性对比(英文)显示文摘研究了6种多氯联苯(PCBs)3,3′,4,4′-四氯联苯(PCB77)、2,3,3′,4,4′-五氯联苯(PCB105)、2,3′,4,4′,5-五氯联苯(PCB118)、3,3′,4,4′,5-五氯联苯(PCB126)、2,3,3',4,4',5-六氯联苯(PCB156)和商业型混合多氯联苯Aroclor1254,两种多溴联苯醚(PBDEs)2,2′,4,4′-四溴二苯醚(PBDE47)、十溴二苯醚(PBDE209)对人类癌细胞生长和斑马鱼脱膜与不脱膜胚胎发育的影响.8种化合物均使用0.01、0.1、1.0、10μmol·L-14个浓度进行1~6d的暴露实验.结果表明,PBDE209在最高浓度10μmol·L-1下对结肠癌细胞HCT116(暴露3d后)和RKO(暴露5d后)具有显著的生长抑制作用,所有化合物均对乳腺癌细胞没有显著影响.相比之下,化合物对受精后5~6h(5~6hpf)的斑马鱼胚胎的毒性效应显得比较明显,而各化合物对胚胎的致畸和致死效应又不相同,其毒性强弱依次为PCB126≈PCB156〉PCB1254(Aroclor1254)〉PBDE47〉PCB77〉PCB105≈PCB118≈PBDE209.其中PBDE209在未脱膜暴毒后均无致畸与致死现象,脱膜暴毒后最高浓度才表现出显著意义的致畸作用,而PBDE47在最高浓度下可产生高达80%的致畸率,这说明胚胎绒毛膜具有有效阻挡大分子物质如PBDE209进入的作用.PCBs的毒性效应与其空间结构密切相关.如PCB126和PCB105具有相同的分子式,前者在1μmol·L-1下就引起了显著的致死和致畸效应,而后者即使在10μmol·L-1下也没有显著的效应.实验结果也说明不同类型的实验对象所展示的毒性效应并不相同,化合物对体外培养的细胞和发育中的胚胎具有不同的影响. | 沈华萍 黄长江 陆芳 杨军华 TANGUAY Robert 孙鲁浙 董巧香 | 2009 | 生态毒理学报2009，4，5： | 11 |
| 4 | Zebrafish embryo toxicity of 15 chlorinated, brominated, and iodinated disinfection by-products显示文摘Disinfection to protect human health occurs at drinking water and wastewater facilities through application of non-selective oxidants including chlorine. Oxidants also transform organic material and form disinfection by-products(DBPs), many of which are halogenated and cyto-and genotoxic. Only a handful of assays have been used to compare DBP toxicity,and researchers are unsure which DBP(s) drive the increased cancer risk associated with drinking chlorinated water. The most extensive data set employs an in vitro model cell,Chinese hamster ovary cells. Traditionally, most DBP research focuses on the threat to human health, but the effects on aquatic species exposed to DBPs in wastewater effluents remain ill defined. We present the developmental toxicity for 15 DBPs and a chlorinated wastewater to a model aquatic vertebrate, zebrafish. Mono-halogenated DBPs followed the in vivo toxicity rank order: acetamides > acetic acids > acetonitriles ~ nitrosamines, which agrees well with previously published mammalian in vitro data. Di-and tri-halogenated acetonitriles were more toxic than their mono-halogenated analogues, and bromine-and iodine-substituted DBPs tended to be more toxic than chlorinated analogues. No zebrafish development effects were observed after exposure to undiluted or non-concentrated,chlorinated wastewater. We find zebrafish development to be a viable in vivo alternative or confirmatory assay to mammalian in vitro cell assays. | David Hanigan Lisa Truong Michael Simonich Robert Tanguay Paul Westerhoff | 2017 | Journal of Environmental Sciences2017，29，8： | 5 |
| 5 | ACOUSTIC MEASUREMENTS BUBBLES IN BIOLOGICAL TIESSURE显示文摘An acoustic based instrument, the ABS Acoustic Bubble Spectrometer?? (ABS), was investigated for the detection and quantification of bubbles in biological media. These include viscoelastic media (blood), materials of varying density (bone in tissue), non-homogenous distribution of bubbles (intravenous bubbly flow), and bubbles migrating in tissue (decompression sickness, DCS). The performance of the ABS was demonstrated in a series of laboratory experiments. Validation of the code was performed using a viscoelastic polymer solution, Polyox, in which the bubble size distribution and void fraction were determined by ABS measurements and with image analysis of high speed videos. These tests showed that the accuracy of the ABS was not significantly affected by viscoelasticity for bubbles smaller than 200 microns. The ABS detection and measurement of non-homogenous bubble distributions was demonstrated using a bubbly flow through a simulated vein surrounded by tissue. The scatter of acoustic signals due to bones in the acoustic pathway was also investigated. These in-vitro experiments were done using meat (beef) as a tissue simulant. Decompression experiments were done using beef meat which was held underwater at high pressure (9.9 atm) then rapidly decompressed. Bubble size distributions and void fraction calculations in these experiments were then validated using image analysis of high speed video. In addition, preliminary experiments were performed with the US Navy Medical Research Center, demonstrating the utility of the modified ABS system in detecting the evolution of bubbles in swine undergoing decompression sickness (DCS). These results indicate that the ABS may be used to detect and quantify the evolution of bubbles in-vivo and aid in the monitoring of DCS. | CHAHINE Georges L. TANGUAY Michel LORAINE Greg | 2009 | Journal of Hydrodynamics2009，21，1： | 3 |
| 6 | Daytime functional bladder capacity as a predictor of response to desmopressin in monosymptomatic nocturnal enuresis显示文摘 | Eller DA Austin PF Tanguay S | 1998 | Eur Urol1998，33，3： | 1 |
| 7 | Heat-shock response and limitation of tissue necrosis during occlusion/reperfusion in rabbit hearts显示文摘 | Currie RW Tanguay RM Kingma JG Jr | 1993 | Circulation1993，87，3： | 1 |
| 8 | Effect of transient gasdynamic processes on the impulse of pulse detonation engines 显示文摘 | Kiyanda C B Tanguay V Higgins A J | 2002 | Journal of Propulsion and Power2002，18，5： | 1 |
| 9 | Coronary artery endothelia protection after local delivery of 17β estradiol during balloon angioplasty in porcine model: a potential new pharmacologic approach to improve endothelial function 显示文摘 | Chandrasekar B Nattel S Tanguay JF | 2001 | J Am Coll Cardiol2001，38，5： | 1 |
| 10 | Doubledose versus standarddose clopidogrel and high-dose versus lowdose aspirin in individuals undergoing percutaneous coronary intervention for acute coronary syndromes(CURRENT-OASIS7):a randomised factorial trial显示文摘 | MEHTA SR TANGUAY JF EIKELBOOM JW | 2010 | Lancet2010，376，12： | 1 |
| 11 | Multiparametric analysis of waterline contamination in dental units显示文摘 | Barbeau J Tanguay R Faucher E | 1996 | Appl Environ Microbiol1996，62，11： | 1 |
| 12 | Haplotype variation and linkage disequilibrium in 313 human genes显示文摘 | Stephens J C Schneider J A Tanguay D A | 2001 | Science2001，293，： | 1 |
| 13 | Leiomyosarcoma of urinary bladder following cyclophosphamide therapy: reporl of two cases显示文摘 | Tanguay C Harvey I Houde M | 2003 | Mod Pathol2003，16，5： | 1 |
| 14 | Histological analysis of acute toxicity of 2,3,7,8-tetra-chlorodibenzop-dioxin(TCDD)in zebrafish显示文摘 | Zodrow I M Stegeman J J Tanguay R L | 2004 | Aquatic Toxicology2004，，66： | 1 |
| 15 | Tissue-specific posttranslational modification of the small heat shock protein HSP27 in Drosophila显示文摘 | Marin R Landry J Tanguay R M | | 0，，01： | 1 |
| 16 | Management of coronary artery diseage:therapeutic options in patients with diabetes显示文摘 | Hammound T Tanguay JF Bouraesa MG | 2000 | J Am Coll Cardiol2000，36，： | 1 |
| 17 | Management of coronary artery disease: therapeutic options in patients with diabetes 显示文摘 | Hammoud T Tanguay J F Bourassa M G | 2000 | J Am Coll Cardiol2000，36，2： | 1 |
| 18 | Coronary Artery Endothelial Protection after Local Delivery of 17beta-estradiol During Ballom Angioplasty in a Porcine Model:a Potential new Pharmacologic Approach to Improve Endothelial Function显示文摘 | CHANDRASEKAR B NATTEL S TANGUAY J F | 2001 | J Am Cell Cardio2001，38，： | 1 |
| 19 | Prevention of in-stent restenosis via reduction of thrombo-inflammatory reactions with recombinant P-selectin glycoprotein ligand-1显示文摘 | Tanguay JF Geoffroy P Sirois MG | 2004 | Thromb Haemost2004，91，6： | 1 |
| 20 | Direct comparison of ELISPOT and ELISA based assays for detection of individual cytokine secreting cells显示文摘 | Tanguay S Killion J | 1994 | Lymphokine Cytokine Res1994，13，4： | 1 |
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