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| 1 | Different approaches to caudate lobectomy with“curettage and aspiration”technique using a special instrument PMOD:A Report of 76 cases显示文摘AIM: To study different approaches to caudate lobectomy with 'curettage and aspiration' technique using Peng's multifunctional operative dissector (PMOD). The surgical procedure of isolated complete caudate lobectomy was specially discussed.METHODS: In 76 cases of various types of caudate lobectomy, three approaches were used including left side approach, right side approach, and anterior approach.Among the 76 cases, isolated complete caudate lobectomy was carried out in 6 cases with transhepatic anterior approach. The surgical procedure consisted of mobilization of the total liver, ligation and separation of the short hepatic veins, splitting the liver parenchyma through the Cantlie's plane, ligation and division of the caudate portal triads from the hilum, dissection of the root of major hepatic veins,detachment of the caudate lobe from liver parenchyma.RESULTS: The mean operative time was 285±51 min,the mean blood loss was 1 600 mi. No severe complications were observed. Among the 6 cases receiving isolated complete caudate lobectomy with transhepatic anterior approach, one case died 17 months after operation due to disease recurrence and liver failure, the other 5 cases have been alive without recurrence, with one longest survival of 49 months.CONCLUSION: The choice of approach is essential to the success of caudate lobectomy. As PMOD and 'curettage and aspiration' technique can delineate intrahepatic or extrahepatic vessels clearly, caudate lobe resection has become safer, easier and faster. | Shu—YouPeng Jiang—TaoLi Yi-PingMou Ying-BinLiu Yu-LianWu He-QingFang Li-PingCao LiChen Xiu-JunCai Cheng-HongPeng | 2003 | World Journal of Gastroenterology2003，9，10： | 29 |
| 2 | Surveillance, epidemiology, and pathogen spectrum of hand, foot, and mouth disease in mainland of China from 2008 to 2017显示文摘Hand,foot and mouth disease(HFMD)was reported in May 2,2008 to be the 38th legally notifiable disease in China's National Notifiable Disease Reporting and Surveillance System.In order to solve the infection,an extensive three-level HFMD surveillance laboratory network was established.In this study,the framework of that network is assessed and the incidence of HFMD in China from 2008 to 2017 is reported using a descriptive epidemiologic method.During these 10 years,a series of techniques have been widely applied in all the network laboratories.Using information and material obtained from the network,a virus bank and database containing 18,238 viruses were established.Nationally,18,184,834 HFMD cases,including 152,436 severe cases and 3633 fatal cases,were reported in mainland of China.The average annual incidence in the population was 133.99/100,000 people,with a maximum incidence of 205.06/100,000 people in 2014.The incidence and mortality rates of HFMD were the highest in children aged 1–2 years.The numbers of reported cases fluctuated,with a high incidence observed every 2 years.An overall increase in the number of reported cases was also observed throughout the study period.Despite this,the incidence of severe cases and the mortality rate have been decreasing.High-risk regions are located in southern,eastern,and central China.Two peaks of HFMD infection cases were observed annually except for Northeast China.Different proportions of enterovirus serotypes were observed during the studied years.The predominant enterovirus varies from year to year,but the disease severity is always closely related to the specific serotype.EV-A71 is the dominant serotype associated with severe and fatal cases,with constituent ratios of 70.03%and 92.23%,respectively.The studied highly sensitive and efficient surveillance network provides information that is critical for prevention and control of the disease.It is extremely necessary and important to continuously conduct extensive virological surveillance for HFMD. | Tianjiao Ji Taoli Han Xiaojuan Tan Shuangli Zhu Dongmei Yan Qian Yang Yang Song Aili Cui Yan Zhang Naiying Mao Songtao Xu Zhen Zhu Dandan Niu Yong Zhang Wenbo Xu | 2019 | Biosafety and Health2019，1，1： | 26 |
| 3 | The antitumor effect of tanshinone IIA on antiproliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line显示文摘The effects of tanshinone IIA on the proliferation of the human non-small cell lung cancer cell line A549 and its possible mechanism on the VEGF/VEGFR signal pathway were investigated.The exploration of the interaction between tanshinone IIA and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs.The CCK-8 assay was used to evaluate the proliferative activity of A549 cells treated with tanshinone IIA(2.5 80 μmol/L) for 24,48 and 72 h,respectively.Flow cytometry was used for the detection of cell apoptosis and cell cycle perturbation.VEGF and VEGFR2 expression were studied by Western blotting.The binding mode of tanshinone IIA within thecrystal structure of the VEGFR2 protein was evaluated with molecular docking analysis by use of the CDOCKER algorithm in Discovery Studio 2.1.The CCK-8 results showed that tanshinone IIA can significantly inhibit A549 cell proliferation in a dose-and time-dependent manner.Flow cytometry results showed that the apoptosis rate of tested group was higher than the vehicle control,and tanshinone IIAtreated cells accumulated at the S phase,which was higher than the vehicle control.Furthermore,the expression of VEGF and VEGFR2 was decreased in Western blot.Finally,molecular docking analysis revealed that tanshinone IIA could be stably docked into the kinase domain of VEGFR2 protein with its unique modes to form H-bonds with Cys917 and π–π stacking interactions with Val848.In conclusion,tanshinone IIA may suppress A549 proliferation,induce apoptosis and cell cycle arrest at the S phase.This drug may suppress angiogenesis by targeting the protein kinase domains of VEGF/VEGFR2. | Jun Xie Jiahui Liu Heng Liu Shihui Liang Meigui Lin Yueyu Gu Taoli Liu Dongmei Wang Hui Gee Sui-lin Mo | 2015 | Acta Pharmaceutica Sinica B2015，5，6： | 26 |
| 4 | Inhibitory effect of topiramate on Lewis lung carcinoma metastasis and its relation with AQP1 water channel显示文摘AIM: To study the effect of topiramate on tumor metastasis and its relation with aquaporin 1 (AQP1) water channel. METHODS: Lewis lung carcinoma metastatic model was used to determine the effect of topiramate on tumor growth and metastasis. Colorimetric estimation was used to investigate the action of topiramate on carbonic anhydrase (CA) activity. Western blotting and immunohistochemical analysis were used to study the influence of topiramate on AQP1 water channel expression in lungs or tumor tissues of mice bearing Lewis lung carcinoma.RESULTS: Treatment with topiramate (120 mg.kg^-1.d^-1, ig for 20 d) reduced the growth of primary tumor signifi-cantly (P<0.05). Its inhibitory rate of metastasis was 81.25 %. Topiramate inhibited CA activity in lungs of mice in a dose-dependent manner. Topiramate apparently decreased AQP1 protein expression and immunostaining in lungs or in tumor microvessel endothelial cells of mice. CONCLUSION: Suppression of AQP1 water channel expression may be an important pathway for the inhibitory effect of topiramate on tumor metastasis. | BingMA YangXIANG TaoLI He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，1： | 25 |
| 5 | Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis显示文摘AIM: To study effects of acetazolamide on aquaporin-1 (AQP1) protein expression and angiogenesis. METHODS: Establishing Lewis-lung-carcinoma model, the localization of AQP1 in tumor tissues was investigated by immuno- histochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. RESULTS: Immunohistochemical localization of AQP1in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAMand endothelial cell proliferation. CONCLUSION: Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis. | YangXIANG BingMA TaoLI Jun-weiGAO He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，6： | 23 |
| 6 | Expression of subtypes of somatostatin receptors in hepatic stellate cells显示文摘AIM: To elucidate the mechanism by which somatostatin and its analogue exert the influence on liver fibrosis, and to investigate the mRNA expression of somatostatin receptors subtypes (SSTRs) and the distribution of somatostatin analogue octreotide in rat hepatic stellate cells (HSCs). METHODS: HSCs were isolated from Sprague Dawley (SD) rats by in situ perfusion and density gradient centrifugation. After several passages, the mRNA expression of 5 subtypes of SSTRs were assessed by reverse transcription-polymerase chain reaction (RT-PCR). HSCs were planted on coverslip and co-cultured with octreotide tagged by FITC. Then the distribution of FITC fluorescence was observed under laser scanning confocal microscope (LSCM) in 12-24 h. RESULTS: There were mRNA expression of SSTR2, SSTR3 and SSTR5 but not SSTR1 and SSTR4 in SD rat HSCs. The mRNA expression level of SSTR2 was significantly higher than that of other subtypes (P<0.01). FITC fluorescence of octreotide was clearly observed on the surface and in the cytoplasm, but not in the nuclei of HSCs under LSCM. CONCLUSION: The effect exerted by somatostatin and its analogues on HSCs may mainly depend on the expression of SSTR2, SSTR3 and SSTR5. Octreotide can perfectlycombine with HSCs, and thereby exerts its biological activity on regulating the characters of active HSCs. This provides a potential prevention and management against liver fibrosis. | Sheng-HanSong Xi-ShengLeng TaoLi Zhi-ZhongQin Ji-RunPeng LiZhao Yu-HuaWei XinYu | 2004 | World Journal of Gastroenterology2004，10，11： | 8 |
| 7 | Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oocyte显示文摘AIM: To study the effects of acetazolamide and anordiol on osmotic water permeability in aquaporin 1 (AQP1)-cRNA injected Xenopus oocyte and their mechanisms. METHODS: AQP1 gene constructed in pBluescript was transcripted into cRNA in vitro and then the cRNA was injected in Xenopus oocytes. The effects of acetazolamide and anordiol on the water transport function of AQP1 were observed by assaying the osmotic swelling of oocytes.In addition, their effects on protein expression of AQP1 were quantitatively investigated by Western blotting method.RESULTS: After incubation for 15 min or 72 h, acetazolamide, a carbonic anhydrase inhibitor, equally reduced the water permeability of AQPI-cRNA injected oocyte in a dose-dependent manner. After incubation for 72 h, anordiol,an antiestrogen with partial estrogenic activity, reduced the osmotic water permeability dose dependently as well;however, no discernable action was observed after incubation with anordiol for 15 min. The Western blotting analysis showed that acetazolamide did not influence the protein expression of AQP1. However, after incubation for 72 h with anordiol (10 μmol/L), the quantity of AQP1 in the oocyte membrane was decreased dramatically(P<0.05). CONCLUSION: Both acetazolamide and anordiol inhibited the osmotic water permeability of AQP1-cRNA injected oocyte, but their mechanisms were different. Acetazolamide functionally inhibited the osmotic water permeability of AQP1, whereas anordiol primarily decreased the amount of AQP1 protein in the oocyte membrane. | BingMA YangXIANG Sheng-meiMU TaoLI He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，1： | 6 |
| 8 | PhieCBEs:Plant High-Efficiency Cytidine Base Editors with Expanded Target Range显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing can efficiently produce gene-knockout mutants.On the other hand,CRISPR/Cas-derived base editors offer the ability to induce precise nucleotide substitutions(Komor et al.,2016).Cytidine base editors(CBEs)consist of a cytidine deaminase fused with a Cas9-nickase variant(Cas9n,with a D10A substitu-tion)and can achieve site-specific C-to-T substitution.Similarly,adenine base editors use an adenine deaminase forA-to-G substi-tution.These systems have been used in various organisms(Mishra et al.,2019).However,the Cas9 complex requires target sites containing NGG protospacer adjacent motifs(PAMs),thus restricting selection of potential targets.A number of CBEs have been developed using Cas9 variants(mostly Cas9n),cytidine deaminases(such as rAPOBEC1 and PmCDA1),and uracil glycosylase inhibitor(UGI)domains.These CBEs of the first generation(BE1,rAPOBEC1-dCas9),second generation(BE2,rAPOBEC1-dCas9-UGI),and third generation(BE3,rAPOBEC1-Cas9n-UGI)have moderate editing efficiencies in mammalians(Komor etal.,2016). | Dongchang Zeng Taoli Liu Jiantao Tan Yaling Zhang Zhiye Zheng Bin Wang Degui Zhou Xianrong Xie Menghui Guo Yao-Guang Liu Qinlong Zhu | 2020 | Molecular Plant2020，13，12： | 5 |
| 9 | Peroxisome proliferator-activated receptor γ ligands suppress liver carcinogenesis induced by diethylnitrosamine in rats显示文摘AIM: Peroxisome proliferator-activated receptor γ (PPARγ) is known to regulate growbh arrest and terminal differentiation of adipocytes and is used clinically as a new class of antidiabetic drugs. Recently, several studies have reported that treatment of cancer cells with PPARγ ligands could induce cell differentiation and apoptosis, suggesting a potential application as chemopreventive agents against carcinogenesis. In the present study, 3 different kinds of PPARγ ligands were subjected to the experiments to confirm their suppressive effects on liver carcinogenesis.METHODS: Three PPARy ligands, pioglitazone (Pio) (200 ppm),rosiglitazone (Rosi) (200 ppm), and troglitazone (Tro) (1 000 ppm) were investigated on the induction of the placental form of rat glutathione S-transferase (rGST P) positive foci, a precancerous lesion of the liver,and liver cancer formation using a diethylnitrosamine-induced liver cancer model in Wistar rats, and dose dependency of a PPART ligand was also examined.RESULTS: PPART ligands reduced the formation of rGST P-positive foci by diethylnitrosamine and induction of liver cancers was also markedly suppressed by a continuous feeding of Pio at 200 ppm.CONCLUSION: PPART ligands are potential chemoprevenvea gents for liver carcinogenesis. | Yan-TongGuo Xi-ShengLeng TaoLi Jing-MingZhao Xi-HouLin | 2004 | World Journal of Gastroenterology2004，10，23： | 4 |
| 10 | mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense显示文摘AIM: To study the immune response of host to Helicobacter pylori VacA.METHODS: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h postinfection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot.RESULTS: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion.CONCLUSION: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response. | Jian-PingYuan TaoLi Zhen-HongLi Gui-ZhenYang Bao-YuHu Xiao-DongShi Tie-LiuShi Shan-QingTong Xiao-KuiGuo | 2004 | World Journal of Gastroenterology2004，10，10： | 4 |
| 11 | Mechanical Properties of Deep-buried Marble Material Under Loading and Unloading Tests显示文摘The mechanical properties are essentially different when rock material is subjected to loading or unloading conditions. In this study, loading and unloading tests with various confining pressures are conducted to investigate the mechanical properties of marble material samples taken from the deep diversion tunnels of Jinping II Hydropower Station. The stress-strain relationship, failure characteristics and strength criterion are compared and analyzed based on the experiment results. The results show: in the loading and unloading test, peak strength, lateral strain, axial strain and plastic deformation increase significantly as the confining pressure increases. Lateral strain increased significantly and obvious lateral dilatancy can be observed to the change of confining pressure; The fracture mode is mainly the single shear fracture for the triaxial compression test and post-peak test, angle between the failure surface and the ends of the rock material becomes smaller as the confining pressure increases. Hoek-Brown strength criterion reflects the strength characteristics of marble material under two different unloading conditions, and has some supplementary effects to the rock material of mechanical field. | 李新平 ZHAO Hang WANG Bin XIAO Taoli | 2013 | Journal of Wuhan University of Technology(Materials Science)2013，28，3： | 2 |
| 12 | Plant Synthetic Metabolic Engineering for Enhancing Crop Nutritional Quality显示文摘Nutrient deficiencies in crops are a serious threat to human health,especially for populations in poor areas.To overcome this problem,the development of crops with nutrient-enhanced traits is imperative.Biofortification of crops to improve nutritional quality helps combat nutrient deficiencies by increasing the levels of specific nutrient components.Compared with agronomic practices and conventional plant breeding,plant metabolic engineering and synthetic biology strategies are more effective and accurate in synthesizing specific micronutrients,phytonutrients,and/or bioactive components in crops.In this review,we discuss recent progress in the field of plant synthetic metabolic engineering,specifically in terms of research strategies of multigene stacking tools and engineering complex metabolic pathways,with a focus on improving traits related to micronutrients,phytonutrients,and bioactive components.Advances and innovations in plant synthetic metabolic engineering would facilitate the development of nutrient-enriched crops to meet the nutritional needs of humans. | Qinlong Zhu Bin Wang Jiantao Tan Taoli Liu Li Li Yao-Guang Liu | 2020 | Plant Communications2020，1，1： | 2 |
| 13 | The mobilization of rat’s mesenchymal stem cells into peripheral blood by LiCL and its potency differentiation显示文摘Mesenchymal stem cells (MSCs) are multipotent cells, which can differentiate into different tissues. It is still controversial whether MSCs can be isolated from adult peripheral blood (PB) under normal conditions and whether they can be mobilized in a way similar to that of hematopoietic stem cells (HSCs). In this study, rat MSCs circulating in the PB under normal conditions can be isolated and cultured, and MSCs from PB and BM can be mobilized by LiCL. The mobilized MSCs can be induced to differentiate into neuron by such factors as β-mercaptoethanol and supernatants of nerve cell cultures. The present study provides a broader perspective on the repair of neural trauma. | DENG dun ZOU ZhongMin ZHOU TaoLi AI GuoPing WANG JunPing DONG ShiWu SU YongPing | 2008 | Chinese Science Bulletin2008，53，17： | 2 |
| 14 | Triterpenoids from the Schisandraceae family显示文摘 | Wei Liexiao Rong Taoli Sheng Xionghuang | 2008 | Natural Product Reports2008，25，5： | 1 |
| 15 | What determines employment opportunity for college graduates in China after higher education reform? 显示文摘 | TaoLi JuyanZhang | 2009 | China Economic Review2009，，211： | 1 |
| 16 | Combination of Images with Diverse Focuses Using the Spatial Frequency显示文摘 | Shu Taoli Kwok J T Wang Yaonan | 2001 | Information Fusion2001，2，3： | 1 |
| 17 | Mechanical properties of deep-buried marble material under loading and unloading tests显示文摘 | Xinping Li Hang Zhao Bin Wang Taoli Xiao | 2013 | Journal of Wuhan University of Technology-Mater. Sci. Ed2013，，3： | 1 |
| 18 | Genotypes Diversity of Acute Gastroenteritis Outbreaks Caused by Human Sapovirus-Beijing Municipality,China,2015-2021显示文摘Introduction:Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of acute gastroenteritis(AGE)globally.A seven-year active surveillance study was conducted to investigate the molecular epidemiology of HuSaVs associated with AGE outbreaks in Chaoyang District of Beijing Municipality,China from January 2015 to December 2021.Methods:Fecal and anal swab samples were obtained from patients experiencing AGE outbreaks.HuSaVs were identified through reverse transcription polymerase chain reaction(RT-PCR),and partial viral protein 1(VP1)sequences(approximately 434 base pairs)were utilized for genotyping,single nucleotide polymorphism(SNP)analysis,and phylogenetic examination.Results:HuSaVs were identified in 71 AGE outbreaks,demonstrating a detection rate of 10.5%,second only to norovirus.The primary demographic affected by HuSaV were children under the age of 5 in kindergarten settings.Infection rates tended to peak during two distinct periods:May to June and September to December.Upon genotyping,seven distinct genotypes emerged.GII.3 was the most prevalent,accounting for 54.9%of cases,followed by GI.1(12.7%),GI.2(9.9%),GII.5(7.0%),GI.5(2.8%),GI.6(1.4%),GII.1(1.4%),and untyped cases(9.9%).A phylogenetic analysis of GII.3 identified three distinct groups,with 15 notable SNPs observed.Conclusions:This study offers a comprehensive analysis of the persistent prevalence of HuSaV outbreaks in Chaoyang District,Beijing Municipality,China.Over time,the diversity of HuSaV subtypes has shifted,and it is now recognized as the second leading viral agent responsible for AGE outbreaks.This highlights the importance of ongoing surveillance in the future. | Yang Jiao Taoli Han Xiao Qi Yan Gao Jianhong Zhao Yue Zhang Beibei Li Zheng Zhang Jialiang Du Lingli Sun | 2023 | China CDC weekly2023，5，28： | 1 |
| 19 | Fabrication and Dielectric Characterization of Advanced BaTiO显示文摘 | Zhi‐MinDang You‐QinLin Hai‐PingXu Chang‐YongShi Sheng‐TaoLi JinboBai | 2008 | Adv Funct Mater2008，，10： | 1 |
| 20 | ResearchontreatmentofamoniaGnitrogenoflandfillleachatebymicroGwavemodifiedzeolite显示文摘 | Shang Ping Liu Taoli Kong Xiangjun | 2010 | NonGMetallicMines2010，33，2： | 1 |
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