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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | Phytochemical and biological activities of Crataegus sinaica growing in Egypt显示文摘Objective:To evaluate the cardiac activity and hepatoprotection of Crataegus sinaica (C.sinaica).Methods:All the isolated compounds were isolated by open-column liquid chromatography(CC) using sephadex LH-20 as stationary phase.Elution of the column was performed with EtOH or MeOH.The phytochemical investigation of the young stem of C.sinaica for the first time together with the leaves and flowers lead to the isolation and identification of quercetin,hyperoside,vitexin-2'-0-rhamnoside,epicatechin,procyanidin B2 and procyanidins CI.Results:Rats treated with the low and high dose of C.sinaica leaves with flowers extract showed 15%and 17%reduction in the heart rate,and reduction in the STsegment by 107%and 57%;respectively.The T-amplitude was decreased by 59%of the high dose extract.On the other hand,the young steins and leaves with flowers extracts of C.sinaica on primary culture of rat hepatocytes monolayer indicated a hepatoprotection for the total extract,ethyl acetate,butanol,and chlor of orm fractions at 100μg/mL,75μg/mL,50μg/mL,and 25μg/mL; respectively.Conclusions:The results of these chemical and biological studies suggest the use of C.sinaica growing in Egypt as a preventive drug against cardiovascular and hepatic diseases. The chemical studies suggest the use of woody young stems as a newly investigated bioactive organ.The extraction of unsaturated fatty acids from the seeds of the plant would serve as a good health and nutritive product. | Alaa Tawfeek Refaat Abdelaaty Abdelaziz Shahat Nermine Ahmed Ehsan Nemat Yassin Faiza Hammouda Elsayed Abou Tabl Shams Imbabi Ismail | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，4： | 2 |
| 3 | Serum cystatin C and tubular urinary enzymes as biomarkers of renal dysfunc- tion in type 2 diabetes mellitus 显示文摘 | Assal HS Tawfeek S Rasheed EA | 2013 | Clin Med Insights Endocrinol Diabetes2013，6，6： | 1 |
| 4 | Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis显示文摘 | Al-Sherbiny MM Farrag AA Fayad MH Makled MK Tawfeek GM Ali NM 2004 | 2004 | Parasitol Res2004，93，2： | 1 |
| 5 | Serum cystatin C and tu- bular urinary enzymes as biomarkers of renal dysfunction in type 2 diabetes mellitus 显示文摘 | Assal HS Tawfeek S Rasheed EA | 2013 | Clin Med Insights Endocrinol Diabetes2013，6，： | 1 |
| 6 | Immunological indicators of morbidity in human schistosomiasis mansoni : role of vascular endo- thelial growth factor and anti-soluble egg antigen IgG4 in disease pro- gression显示文摘 | Tawfeek GM Alafifi AM Azmy MF | 2003 | J Egypt Soc Parasitol2003，33，2： | 1 |
| 7 | Establishment of human clones producing neutralizing humanmonoclonal antibodies to the envelope E1/E2 protein of hepatitis C virus by EBVimmortalization of immune CD22+ B cells显示文摘 | Ashraf Tabll Yasmine El Abd Noha G. Bader El Din Reem El Shenawy Tawfeek H. Abdelhafez Mostafa El Awady Hanan El-Mohamady Sergei Viazov | | 2014 (3,4)0，，： | 1 |
| 8 | Evaluation of an IgG cystatin capture enzyme-linked immunosorbent assay for the detection of anti-cysteine proteinases antibodies in asymptomatic trichomoniasis patients显示文摘 | Tawfeek GM Oteifa NM E1-Gozamy BR | 2003 | J Egypt Soc Parasitol2003，33，1： | 1 |
| 9 | Comparative analysis of the diagnostic performance of crude sheep hydatid cyst fluid, purified antigen B and its subunit( 12 Kda) ,assessed by ELISA, in the diagnosis of human cystic echinococcosis显示文摘 | Gihan M Tawfeek Hala S | 2011 | Parasitol Res2011，108，： | 1 |
| 10 | Serum Cystatin C and Tubular Urinary Enzymes as Biomarkers of Renal Dysfunction in Type 2 Diabetes Mellitus显示文摘 | Assal H S Tawfeek S Rasheed E A | 2013 | Clin Med Insights Endoerinol Diabe tes2013，6，： | 1 |
| 11 | Efficacy of an infant formula containing anti-E, coli colostral antibodies from hyperimmunized cows in preventing diarrhea in infants and children: a field trial 显示文摘 | Tawfeek H I Najim N H A1-Mashikhi S | 2003 | Int J Infect Dis2003，7，2： | 1 |
| 12 | Comparative analysis of the diagnostic performance of crude sheep hydatid cyst fluid, purified antigen B and its subunit (12 kDa), as- sessed by ELISA, in the diagnosis of human cystic echinococco- sis显示文摘 | Tawfeek GM Elwakil HS E1-Hoseiny L | 2011 | Parasitol Res2011，108，2： | 1 |
| 13 | Disruption of PTH receptor 1 in T cells protects against PTH-induced bone loss显示文摘 | Tawfeek H Bedi B Li JY | 2010 | PLoS One2010，5，12： | 1 |
| 14 | Efficacy of an infant formula containing anti-E, coli colostral antibodies from hyperimmunized cows in preventing diarrhea in infants and children: A field trial 显示文摘 | Tawfeek H l Najim N H AI-Mashikhi S | 2003 | International Journal of Infectious Diseases2003，，7： | 1 |
| 15 | Printing cotton fabrics with reactive dyes of high reactivity from an acidic print- ing paste显示文摘 | EI-Hamaky Y H Tawfeek S Ibrahim D F | 2007 | Coloration Technology2007，123，6： | 1 |
| 16 | Ef- ficacy of an infant formula containing anti-Escherichia coli colostral antibodies from hyperimmunized cows in preventing diarrhea in infants and children:a field trial显示文摘 | TAWFEEK H I NAJIM N H AI-MASHIKNSI S | 2003 | International Journal of Infectious Diseases2003，7，2： | 1 |
| 17 | Is unilateral transversus abdominis plane block an analgesic alternative for ureteric shock wave lithotripsy显示文摘 | Elnabtity AM Tawfeek MM Keera AA | 2015 | Anesth Essays Res2015，9，1： | 1 |
| 18 | Neutralization of the acidified seawater effluent from the flue gas desulfurization process: Experi- mental investigation, dynamic modeling, and simu- lation显示文摘 | TOKUMURA M BABA M TAWFEEK H | 2006 | Industrial b- Engineering Chemistry Re- search2006，45，18： | 1 |
| 19 | Serum cystatin C and tubular urinary enzymes as biomarkers of renal dysfunction in type 2diabetes mellitus显示文摘 | Assal HS Tawfeek S Rasheed EA | 2013 | Clin Med Insights Endocrinol Diabetes2013，6，： | 1 |
| 20 | Ovariectomy disregulatesosteoblast and osteoclast formation through the T-cell receptorCD40 ligand 显示文摘 | Li JY Tawfeek H BediB | 2011 | Proc Natl Acad Sci USA2011，108，2： | 1 |
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