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| 1 | Isolation, screening and identifi cation of Bacillus spp. as direct-fed microbial candidates for aflatoxin B_1 biodegradation显示文摘Objective: To evaluate the ability of Bacillus spp. as direct-fed microbials(DFM) to biodegrade al atoxin B1(AFB1) by using an in vitro digestive model simulating in vivo conditions.Methods: Sixty-nine Bacillus isolates were obtained from intestines, and soil samples were screened by using a selective media method against 0.25 and 1.00 μg/m L of AFB1 in modii ed Czapek-Dox medium. Plates were incubated at 37 °C and observed every two days for two weeks. Physiological properties of the three Bacillus spp. candidates were characterized biochemically and by 16 S r RNA sequence analyzes for identii cation. Tolerance to acidic p H, osmotic concentrations of Na Cl, bile salts were tested, and antimicrobial sensitivity proi les were also determined. Bacillus candidates were individually sporulated by using a solid fermentation method and combined. Spores were incorporated into 1 of 3 experimental feed groups: 1) Negative control group, with unmedicated starter broiler feed without AFB1; 2) Positive control group, with negative control feed contaminated with 0.01% AFB1; 3) DFM treated group, with positive control feed supplemented with 109 spores/g. After digestion time(3:15 h), supernatants and digesta were collected for high-performance liquid chromatography l uorescence detection analysis by triplicate.Results: Three out of those sixty-nine DFM candidates showed ability to biodegrade AFB1 in vitro based on growth as well as reduction of l uorescence and area of clearance around each colony in modii ed Czapek-Dox medium which was clearly visible under day light after 48 h of evaluation. Analysis of 16S-DNA identii ed the strains as Bacillus amyloliquefaciens, Bacillus megaterium and Bacillus subtilis. The three Bacillus strains were tolerant to acidic conditions(p H 2.0), tolerant to a high osmotic pressure(Na Cl at 6.5%), and were able to tolerate 0.037% bile salts after 24 h of incubation. No signii cant dif erences(P > 0.05) were observed in the concentrations of AFB1 in neither the supernatants nor digesta samples evaluated by highperformance liquid chromatography with l uorescence detection between positive control or DFM treated groups. Conclusions: In vitro digestion time was not enough to confirm biodegradation of AFB1. Further studies to evaluate the possible biodegradation ef ects of the BacillusDFM when continuously administered in experimentally contaminated feed with AFB1, are in progress. | Rosario Galarza-Seeber Juan David Latorre Xochitl Hernandez-Velasco Amanda Drake Wolfenden Lisa Renee Bielke Anita Menconi Billy Marshall Hargis Guillermo Tellez | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，9： | 3 |
| 2 | Complications of Fibrin Glue in Pterygium Surgery with Amniotic Membrane Transplant显示文摘Purpose:To compare the complications and the recurrence rate between fibrin glue (TISSEEL) and Vicryl 8-0 sutures in amniotic membrane transplant during pterygium surgery.Methods:Patients who underwent pterygium surgery with amniotic membrane transplant (AMT) in the Wang Eye Clinic (Doraville,USA).were randomly categorized into two groups:one group received TISSEEL and the other group received Vicryl 8-0 sutures.All procedures for both groups were performed by one ophthalmologist.Twenty-four patients (24 eyes) participated in the TISSEEL group (22 eyes with primary surgery and 2 eyes with surgery for recurrence) and 19 patients (19 eyes) took part in the suture group (18 primary eyes and 1 recurrence).The patients with recurrent pterygium in both groups had not received AMT previously.Post-operatively,patients were followed up for one week and then one year to check for any signs of complications and recurrence.Results:Conjunctival inflammation occurred in 3 eyes (12.5%) in the TISSEEL group,and 6 eyes (31.6%) in the suture group.(P<0.05,Chi-square test).In the TISSEEL group,1 eye (4.2%) showed recurrence,while there were no recurrences in the sutures group.There were 3 dry eyes (15.8%) in the suture group and 2 dry eyes (8.3%) in the TISSEEL group (P>0.05).No dislocated conjunctival grafts,Dellen,inflammation,infection,bleeding,pyogenic granuloma,or scleromalacia was observed in either group.Conclusion:Pterygium surgery with AMT had less conjunctival inflammation and dry eye in patients receiving fibrin glue than those with Vicryl 8-0 sutures. | Lan P.Nguyen Zhengxiang Wang Jesley Molina Andrea Tellez Tek Chemoriya | 2012 | Eye Science2012，27，1： | 2 |
| 3 | A local spatial decision support system for developing countries based on MCA,fuzzy sets and OWA–case study of a municipality in Cuba显示文摘This paper presents a Spatial Decision Support System for local governments of developing countries.It allows municipality government,enterprises,scientific community and civil society to address decision problems using GIS.The framework is supported by four modules of information technologies:Environmental Decision Support Database,Data Manipulation,Decision Support,and Mapping.A case study is presented covering the implementation of this framework in one municipality of Cuba.An example of land suitability planning for coconut crops is used to evaluate the system performance and usability.Results show local municipalities are able to use this framework to solve local decision problems using state of the art decision making even with low infrastructure development. | Ricardo DELGADO TELLEZ ZHONG Ershun ZUHU Yang Arisleydis PEÑA DE LA CRUZ | 2013 | Geo-Spatial Information Science2013，16，2： | 2 |
| 4 | Hydrolysis of sorghum straw using phosphoric acid: Evaluation of furfural production 显示文摘 | VAZQUEZ M OLIVA M TELLEZ L | 2007 | Bioresource Technology2007，98，16： | 1 |
| 5 | Fungicidal potential of methoxylated flavones from citrus for in vitro control of Colletotrichum loeosporioides, causal agent of anthracnose disease in tropical fruits 显示文摘 | Almada Ruiz E Martinez Tellez M A Hem ndez Alamos M M | 2003 | Pest Manag Sci2003，59，： | 1 |
| 6 | Application of Advanced Dy-namics Modeling Significantly Improves the Stability ofRotary Steerable Drilling with Roller Reamer 显示文摘 | Carlos Perez Tellez | 2009 | IPTC139782009，，： | 1 |
| 7 | Fully human antibodies to MCAM/ MUC18 inhibit tumor growth and metastasis of human melanoma显示文摘 | Mills L Tellez C Huang S | | 0，，17： | 1 |
| 8 | GYY4137,a novel hydrogen sulfide-re- leasing molecule,protects against endotoxie shock in the rat显示文摘 | Li LSalto Tellez M Tan CH | 2009 | Free Radie Biol Med2009，47，1： | 1 |
| 9 | Performance evaluation of an activated sludge system for removing petroleum hydrocarbons from oilfield produced water 显示文摘 | Tellez G T Nirmalakhandan N Jorge G L | 2002 | Advances in Environ- mental Research2002，，6： | 1 |
| 10 | Phase II trial ofehemoembo- lization for the treatment to metastatic carcinoma to the liver and rev- iew of the literature显示文摘 | Tellez C Benson AB Lyster MT | 2008 | Cancer2008，82，7： | 1 |
| 11 | Nursing Informatics Education Past, Present, and Future显示文摘 | Michelle Tellez | 2012 | CIN: Computers, Informatics, Nursing2012，，5： | 1 |
| 12 | Phase Ⅱ trial of ehemoemboli- zation for the treatment to metastatic colorectal carcinoma to the liver and review of the literature显示文摘 | Tellez C Benson AB Lyster MT | 1998 | Cancer1998，82，7： | 1 |
| 13 | Risk factors associated with depression in patients with Ⅱ diabetes mellitus 显示文摘 | Tellez JF Cardiel MH | 2002 | Arch Med Res2002，33，1： | 1 |
| 14 | Extended a- trial conduction system characterized by the expression of the HCN4 channel and connexin45 显示文摘 | Yamamoto M Dobrzynski H Tellez J | 2006 | Cardiovasc Res2006，72，2： | 1 |
| 15 | Iber-assisted self- diverting acid brings a new perspective to hot, deep carbonate stimulation in mexico 显示文摘 | F Martin M Quevedo F Tellez F | 2010 | SPE 1389102010，，： | 1 |
| 16 | Controllability of heat exchanger networks 显示文摘 | Rodolfo Tellez William Y Svrcek Brent R Young | 2006 | Heat Transfer Engineering2006，27，6： | 1 |
| 17 | Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma显示文摘 | Mills L Tellez C Huang S | 2002 | Cancer Res2002，62，： | 1 |
| 18 | Serum levels of IL-lbeta, IL-6and TNF-alpha in infertile patients with o- varian dysfunction 显示文摘 | Vital Reyes VS Tellez Velasco S Hinojosa Cruz JC | 2005 | Ginecol Obstet Mex2005，73，60： | 1 |
| 19 | Autoradiographic study of serotonin transporter during memory formation显示文摘 | Tellez R Rocha L Castillo C Meneses A | | 0，，： | 1 |
| 20 | Complications of epilepsy surgery—A systematic review of focal surgical resections and invasive EEG monitoring显示文摘 | Walter J. Hader Jose Tellez‐Zenteno Amy Metcalfe Lisbeth Hernandez‐Ronquillo Samuel Wiebe Churl‐Su Kwon Nathalie Jette | 2013 | Epilepsia2013，，5： | 1 |
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