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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Acid- and basecatalyzed hydrolysis of chloroacetamide herbicides 显示文摘 | Carlson D L Than K D Roberts A L | 2006 | Journal of Agricultural and Food Chemistry2006，53，16： | 1 |
| 3 | Successful hematopoietic reconstitution with transplantation of erythrocyte-depleted allogeneic human umbilic cord blood cells in a child with leukemia显示文摘 | PAHWA RN FLEISCHER A THAN S | 1994 | Proc Natl Acad Sci1994，91，： | 1 |
| 4 | Distribution of epilithic di- atoms in response to environmental conditions in an urban tropical stream,Central Kenya 显示文摘 | George G Diritu A Than N | 2006 | Biodiversity and Con- servation2006，15，： | 1 |
| 5 | Graphene quantum dots as universal fluorophores and their use in re- vealing regulated traflqcking of insulin receptors in adipocytes 显示文摘 | Zheng XT Than A AnanthanarayaA eta/ | 2013 | ACS Nano2013，7，7： | 1 |
| 6 | Measuring modularity - based manufacturing practices and their impact on mass customization capa- bility: a customer - driven perspective 显示文摘 | Tu Q Vonderembse M A Ragn Nathan T S Ragu Na- than B | 2004 | Decision Sciences2004，35，2： | 1 |
| 7 | Chemorheological study of a dicyanate ester for the simulation of the resintransfer molding process 显示文摘 | Maazouz A Texier C Than M | 1998 | Composites Science and Technology1998，58，5： | 1 |
| 8 | Enterobacter sakazakii bacteraemia with multiple splenic abscesses in a 25-year-old woman:a case report显示文摘 | SEE K C THAN H A TANG T | 2007 | Age and Ageing2007，36，： | 1 |
| 9 | Safety, immunogenicity and efficacy of an alphavirus replicon- based swine influenza virus hemagglutinin vaccine显示文摘 | Ryan L Vander Veen Alan T Loyna than Mark A Mogler | 2012 | Vaccine2012，30，： | 1 |
| 10 | Review of the lubrication of metallic surfaces by zinc dialkyl-dithiophates显示文摘 | Mark A Nicholls Than Do Peter R Norton | 2005 | Tribology International2005，38，： | 1 |
| 11 | Chemical and mechanical properties of ZDDP antiwear films on steel and thermal spray coatings studied by XANES spectroscopy and nanoindentation techniques显示文摘 | Mark A N Than D Peter R N | 2003 | Tribology Letters2003，15，3： | 1 |
| 12 | Monochioamine inhibits the expression of E - selectin and intercellular adnesion molecute - 1 in duced by TNF - alpha through the suppression of NF - KB activation in human endothelials cell 显示文摘 | OMORI M OGINO T THAN T A | 2002 | Free Radic Res2002，，： | 1 |
| 13 | Reduction of CuO in Hz : in situ time- resolved XRD studies 显示文摘 | RODRIGUEZ JSOI~ A KIM JAE Y HANSON JONA- THAN C | 2003 | Catalysis Letters2003，85，3： | 1 |
| 14 | Early Dynamic Change in High-Sensitivity Cardiac Troponin T in the Investigation of Acute Myocardial Infarction显示文摘 | Aldous Sally J Richards A Mark Cullen Louise Than Martin P | 2011 | EN2011，，： | 1 |
| 15 | Molecular identification of Ectomycorrhizal mycelium in soil horizons 显示文摘 | Renske L Paul A L Than W K | 2003 | Appl Environ Microbiol2003，69，1： | 1 |
| 16 | Heat Exchanger Design显示文摘 | Than S T M Lin K A Mon M S | 2008 | World A- cademy of Science Engineering and Technology2008，46，： | 1 |
| 17 | Aniseikonia testing in an adult population using a new computerized test,the Aniseikonia Inspector显示文摘 | Codiss D A Rutstein R P Than T P et aI | 2005 | Binocul Vis Strabismus Q2005，20，4： | 1 |
| 18 | Acid- and base-catalyzed hydrolysis of chlomacetamide herbicides 显示文摘 | Carlson D L Than K D Roberts A L | 2006 | Journal of Agricultural and Food Chemistry2006，54，13： | 1 |
| 19 | Micromechanics Of Ti-10V-2Fe-3Al:In-situ synchrotron characterisation and modeling显示文摘 | Raghuna Than S L Stapleton A M Dashwood R J Jackson M Dye D | 2007 | Acta Mateialia2007，，55： | 1 |
| 20 | Acid-and basic-catalyzed hydrolysis of chloroacetamide herbicides显示文摘 | CARLSON D L THAN K D ROBERTS A L | 2006 | Journal of Agricultural and Food Chemistry2006，54，： | 1 |
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