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您的检索式：作者名="Timoney John"
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| 1 | 马链球菌Se39.612蛋白的克隆与表达显示文摘马腺疫是一种高度接触性疫病,困扰着发达国家的养马业。通过筛选马链球菌CF32株3kb~8kb随机基因组文库,获得一株阳性克隆。测序拯救质粒得到序列,确定插入片段编码完整aa的分子量为39.612Ku(Se39.612),根据序列设计特异性引物PCR扩增该基因,分子克隆表达该蛋白。用纯化Se39.612蛋白检测马腺疫康复血清及鼻腔冲洗液,表明Se39.612在体液免疫及粘膜免疫中起作用。 | 刘杰 杨建德 Timoney John | 2007 | 中国预防兽医学报2007，29，3： | 2 |
| 2 | Cloning, Expression, and Homology Modeling of GroEL Protein from Leptospira interrogans Serovar Autumnalis Strain N2显示文摘Leptospirosis is an infectious bacterial disease caused by Leptospira species. In this study, we cloned and se- quenced the gene encoding the immunodominant protein GroEL from L. interrogans serovar Autumnalis strain N2, which was isolated from the urine of a patient during an outbreak of leptospirosis in Chennai, India. This groEL gene encodes a protein of 60 kDa with a high degree of homology (99% similarity) to those of other leptospiral serovars. Recombinant GroEL was overexpressed in Escherichia coli. Immunoblot analysis indi- cated that the sera from confirmed leptospirosis patients showed strong reactivity with the recombinant GroEL while no reactivity was observed with the sera from seronegative control patient. In addition, the 3D structure of GroEL was constructed using chaperonin complex cpn60 from Thermus thermophilus as template and vali- dated. The results indicated a Z-score of -8.35, which is in good agreement with the expected value for a pro- tein. The superposition of the Cα traces of cpn60 structure and predicted structure of leptospiral GroEL indi- cates good agreement of secondary structure elements with an RMSD value of 1.5 . Further study is necessary to evaluate GroEL for serological diagnosis of leptospirosis and for its potential as a vaccine component. | Kalimuthusamy Natarajaseenivasan Santhanam Shanmughapriya Sridhar Velineni Sergey C. Artiushin John F. Timoney | 2011 | Genomics, Proteomics & Bioinformatics2011，9，4： | 0 |
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