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| 1 | 小麦农杆菌介导转基因植株的稳定获得和检测显示文摘以预培养 4 d的小麦幼胚愈伤组织为受体 ,L B培养基上激活培养 3 d或 AB培养基上激活培养 4 d,并在WCC培养基中重悬的 C5 8c1农杆菌菌系为供体 ,将含有目的基因、标记基因的 p PTN2 4 9、p PTN 2 5 4、p PTN2 70、p SIS-GFP等重组 DNA质粒转入了小麦 ,经在含 10~ 5 0 mg/ L geneticin( G4 18)、5 0 mg/ L cefotaxime、5 0 m g/ L vancomycin、5 0 m g/ L ticillicin选择培养基上多次选择 ,移栽前利用 npt EL ISA检测 ,移栽后利用叶片离体退绿、PCR和 South-ern blot 4种方法综合检测 ,共获得了 2 9株转基因植株 ,转化频率平均为 0 .82 %。在所获得的转基因植株中 ,单拷贝整合植株占 65 .5 2 %。研究结果还表明 ,不同基因型的转化效率显著不同 ,除了 Bobwhite外 ,笔者也从扬麦 10号获得了转基因植株 ;EL ISA测定值的高低与外源 DNA整合的拷贝数有一定关系 ;初步建立了农杆菌介导稳定转化小麦的技术体系。 | 叶兴国 Shirley Sato 徐惠君 杜丽璞 Tom Clemente | 2001 | 中国农业科学2001，34，5： | 66 |
| 2 | BCL、RIP细胞凋亡基因向小麦中的导入和赤霉病抗性鉴定显示文摘利用农杆菌介导法将BCL和RIP 2个与细胞凋亡有关的基因分别导人了小麦品种扬麦10号和Bobwhite,转化效率分别为1.60%和1.25%。Southern检测结果表明,细胞凋亡基因已稳定整合到小麦染色体上,多数植株为单拷贝整合。Northern检测结果表明,细胞凋亡基因能够在小麦遗传背景中高水平转录为RNA。转基因植株T1代分离比例为2.11~2.33:1,表明外源基因在后代中能够稳定遗传。BCL转基因植株和RIP转基因植株对赤霉病均表现出一定抗性,其中BCL-20、BCL-21、RIP-8、RIP-18等15个T1代植株的小穗发病率为5.6%~16.1%,可望进一步培育抗赤霉病小麦新材料。 | 叶兴国 Shirley Sato 徐惠君 杜丽璞 黄益洪 陆维忠 Tom Clemente | 2005 | 作物学报2005，31，11： | 12 |
| 3 | Experimental-computational study of fibrous particle transport and deposition in a bifurcating lung model显示文摘 | Xiaole Chen Wenqi Zhong Josin Tom Clement Kleinstreuer Yu Feng Xiaopu He | 2016 | Particuology2016，14，5： | 3 |
| 4 | Regular Production of Transgenic Wheat Mediated by Agrobacterium tumefaciens显示文摘Wheat is the number one crop both in acreage and total yield in the world. Therefore, it is very important to improve wheat by gene engineering techniques even though it belongs to the plants insensitive to gene transformation, especially to Agrobacterium-mediated transformation. Wheat immature embryos of 1.0-1.5mm in size, C58c1 of Agrobacterium strain harboring pPTN249, pPTN270, pPTN254, and pSIS-GFP respectively (all the vectors contain the aphA selectable gene driven by enhanced 35S promoter and a target gene controlled by ubi promoter or E35S promoter), AB medium for Agrobacterium activate culture, WCC medium for co-culture, were used in this study. The embryos with 4 days of pre-culture were transferred onto selection medium with 10mg/L geneticin, 50mg/L ticarcillin, 50mg/L vancomycin, and 50mg/L cefotaxine after 30 minutes of infection and 2 days of co-cultivation with Agrobacterium. Followed callus production,shoot regeneration on selection medium, 114 resistant plantlets were obtained from 10 transformation experiments of four genotypes. By nptⅡ ELISA (nptⅡ enzyme assay), PCR, Southern blot and leaf bleach,29 positive plants were identified from two genotypes of Bobwhite and Yanglmai 10, with an average transformation efficiency of 0.82 %. The result tested by Southern blot also showed that the transgenic plants with single- copy integration of target gene took 65.52% among total positive plants. The ELISA value of transgenic plant was also related to the copies of alien DNA integrating into wheat chromosomes, the transgenic plants with single copy integration giving higher ELISA value than the ones with 2 or 3 copies integration. | Tom Clemente | 2002 | Agricultural Sciences in China2002，1，3： | 3 |
| 5 | Payments for biodiversity conservation in the context of weak institutions: Comparison of three programs from Cambodia显示文摘 | Tom Clements Ashish John Karen Nielsen Dara An Setha Tan E.J. Milner-Gulland | 2009 | Ecological Economics2009，，： | 1 |
| 6 | Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybean显示文摘 | Helene Eckert Brad LaVallee Bruce J. Schweiger Anthony J. Kinney Edgar B. Cahoon Tom Clemente | 2006 | Planta2006，，5： | 1 |
| 7 | An evaluation of dengue type-2 inactivated, recombinant subunit, and live-attenuated vaccine candidates in the rhesus macaque model显示文摘 | J. Robert Putnak Beth-Ann Coller Gerald Voss David W. Vaughn David Clements Iain Peters Gary Bignami Hou-Shu Houng Robert C.-M. Chen David A. Barvir Jitvimol Seriwatana Sylvie Cayphas Nathalie Gar?on Dirk Gheysen Niranjan Kanesa-thasan Mike McDonell Tom H | 2005 | Vaccine2005，，35： | 1 |
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