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| 1 | Mining and validation of novel genotyping-bysequencing(GBS)-based simple sequence repeats(SSRs)and their application for the estimation of the genetic diversity and population structure of coconuts(Cocos nucifera L.)in Thailand显示文摘Coconut(Cocos nucifera L.)is an important economic crop in tropical countries.However,the lack of a complete reference genome and the limitations of usable DNA markers hinder genomic studies and the molecular breeding of coconut.Here,we present the results of simple sequence repeat(SSR)mining from a high-throughput genotyping-bysequencing(GBS)study of a collection of 38 coconut accessions.A total of 22,748 SSRs with di-,tri-,tetra-,penta-and hexanucleotide repeats of five or more were identified,2451 of which were defined as polymorphic loci based on locus clustering in 38 coconut accessions,and 315 loci were suitable for the development of SSR markers.One hundred loci were selected,and primer pairs for each SSR locus were designed and validated in 40 coconut accessions.The analysis of 74 polymorphic markers identified between 2 and 9 alleles per locus,with an average of 3.01 alleles.The assessment of the genetic diversity and genetic relationships among the 40 coconut varieties based on the analysis of population structure,principal coordinate analysis(PCoA),and phylogenetic tree analysis using the 74 polymorphic SSR markers revealed three main groups of coconuts in Thailand.The identified SSR loci and SSR markers developed in this study will be useful for the study of coconut diversity and molecular breeding.The SSR mining approach used in this study could be applied to other plant species with a complex genome regardless of the availability of reference genome. | Kanamon Riangwong Samart Wanchana Wanchana Aesomnuk Chatree Saensuk Phakchana Nubankoh Vinitchan Ruanjaichon Tippaya Kraithong Theerayut Toojinda Apichart Vanavichit Siwaret Arikit | 2020 | Horticulture Research2020，7，1： | 4 |
| 2 | Quantitative trait loci associated with drought tolerance at reproductive stage in rice 显示文摘 | Lanceras J C Pantuwan G Jongdee B Toojinda T | 2004 | Plant Physiology2004，135，1： | 1 |
| 3 | Introgression of quantitative trait loci (QTLs) determining stripe rust resistance in barley :anexample of marker- assisted line development显示文摘 | Toojinda T Baird E Booth A | 1998 | Theor Appl Genet1998，96，： | 1 |
| 4 | Quantitative trait loci associated with drought tolerance at reproductive stage in rice 显示文摘 | Lanceras J C Pantuwan G Jongdee B Toojinda T | 2004 | Plant Physiol2004，135，1： | 1 |
| 5 | Ascherxanthone B from Aschersonia luteola, a new antifungal compound active against rice blasl pathogen Magnaporthe grisea 显示文摘 | Chutrakul C Boonruangprapa T Suvannakad R Isaka M Sirithunya P Toojinda T Kirtikara K | 2009 | JAppl Microbiol2009，107，5： | 1 |
| 6 | Evaluation of genetic diversity among soybean genotypes using SSR and SNP显示文摘 | P Tanya P Srinives T Toojinda | 2001 | Korean J Crop Sci2001，46，4： | 1 |
| 7 | Development of elite indica rice lines with wide spectrnm of resistance to Thai blast isolates by pyramiding multiple resistance QTLs 显示文摘 | Sreewongchai T Toojinda T Thanintorn N | 2010 | Plant breeding2010，129，： | 1 |
| 8 | Molecular genetics of submergence tolerance in rice: QTL analysis of key traits 显示文摘 | Toojinda T Siangliw M Tragroonrung S | 2003 | Annals of Botany2003，91，： | 1 |
| 9 | Microsatellite markers flanking the tins2 gene facilitated tropical TGMS rice line development显示文摘 | LOPEZ M T TOOJINDA T VANAVICHIT A | 2003 | Crop Science2003，43，： | 1 |
| 10 | Introgression of quantita- tive trait loci(QTLS)determining stripe rust resistance in bar- ley, An example of marker-assisted line development 显示文摘 | Toojinda T Baird E Booth A | 2004 | Theoretical and Applied Genetics2004，96，： | 1 |
| 11 | Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)显示文摘 | T. Toojinda L. H. Broers X. M. Chen P. M. Hayes A. Kleinhofs J. Korte D. Kudrna H. Leung R. F. Line W. Powell L. Ramsay H. Vivar R. Waugh | 2000 | TAG Theoretical and Applied Genetics2000，，4： | 1 |
| 12 | Thai jasmine rice carrying QTLch9 (SubQTL) is submergence tolerant显示文摘 | Siangliw M Toojinda T Tragoonrung S Vanavichit A | | 0，，02： | 1 |
| 13 | Evaluation of Agronomic Traits in Chromosome Segment Substitution Lines of KDML105 Containing Drought Tolerance QTL under Drought Stress显示文摘Drought is a major abiotic constraint to rice production in rainfed lowland and insufficiently irrigated areas.The improvement of drought tolerant varieties is one of the strategies to reduce the negative effects of drought.Quantitative trait loci(QTLs) for primary and secondary traits related to drought tolerance(DT) on chromosomes 1,3,4,8 and 9 that determined from double haploid lines derived from a cross between CT9993 and IR62266 were introgressed and dissected into small pieces in the genetic background of Khao Dawk Mali 105(KDML105) to develop chromosome segment substitution line(CSSL) population.The CSSLs were evaluated at the reproductive stage for their agronomic performance and yield components under drought stress,and results were compared with irrigated condition.The flowering of CSSL lines was 6 to 7 d earlier than KDML105.The mean values of grain yields in the CSSLs were higher than KDML105 under drought and irrigated conditions.At irrigated condition,the grain yields of introgression lines carrying DT-QTLs from chromosomes 4 and 8 were higher than that of KDML105,whereas other traits showed little difference with KDML105.Analysis indicated that grain yield has positive correlation with plant height,tiller and panicle number per plant,and total grain weight per plant under drought stress while negatively correlated with days to flowering.As mentioned above,CSSLs showing good adaptation under drought stress can be used as genetic materials to improve drought tolerance in Thai rainfed lowland rice breeding program,and as materials to dissect genes underlying drought tolerance. | Vaiphot KANJOO Kanchana PUNYAWAEW Jonaliza L.SIANGLIW Suwat JEARAKONGMAN Apichart VANAVICHIT Theerayut TOOJINDA | 2012 | Rice science2012，19，2： | 1 |
| 14 | ~ai jasmine rice carrying QTLchg(SubQrrL) is submergence tolerant显示文摘 | SIANGLIW M TOOJINDA T TRAGOONRUNG S | 2003 | Annals of Bota- ny2003，91，： | 1 |
| 15 | Molecular bre- eding for rainfed lowland rice in the Mekong region 显示文摘 | TOOJINDA T TRAGOONRUNG S VANAV1CHIT A | 2005 | Plant Prod Sci2005，8，3： | 1 |
| 16 | Characterization of Myanmar Paw San Hmwe Accessions Using Functional Genetic Markers显示文摘Paw San Hmwe(PSM) rice has been cultivated in many areas of Myanmar for a long time. Strong aroma,good taste and its elongation during cooking are the key characteristics of PSM rice. Thirty-one PSM accessions were genotypically characterized,and their physical grain and cooking quality traits were studied. We used specific gene markers associated with aroma,apparent amylose content(AAC) and alkali spreading value to determine the alleles carried by different PSM accessions. The results revealed that six PSM accessions(PSM10,PSM12,PSM13,PSM21,PSM22 and PSM30) had a 3-bp insertion in Os2 AP gene. Gel consistency(GC) allele was predominant among the PSM accessions for gelatinization temperature(GT),however,the phenotype observed was between low and intermediate GT because of the combination of the GC allele with the presence of low GT allele at heterozygous state from the other loci of the SSIIa gene. Intermediate to high AAC was observed among the PSM accessions corresponding to the haplotype identified for the single nucleotide polymorphism G/T and the(CT)n repeat in the Wx gene. The characterization and grouping data of PSM accessions posted benefits to Myanmar seed banks,and our results will help in maintaining the integrity of PSM rice variety. | Kyaw Swar Oo Alisa KONGJAIMUN Srisawat KHANTHONG Myint YI Tin Tin MYINT Siriporn KORINSAK Jonaliza Lanceras SIANGLIW Khin Myo MYINT Apichart VANAVICHIT Chanate MALUMPONG Theerayut TOOJINDA | 2015 | Rice science2015，22，2： | 0 |
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