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| 1 | Homocysteine mediates transcriptional changes of the inflammatory pathway signature genes in human retinal pigment epithelial cells显示文摘AIM:To test whether homocysteine(Hcy) can influence the transcriptional profile, we hypothesized that Hcy can lead to the induction of proinflammatory molecules in the retinal cells of aging people.METHODS:An unbiased in vitro inflammatory pathway focused study was designed employing retinal pigment epithelial(RPE) cell line, ARPE-19. Cells were cultured in the presence or absence of Hcy to capture target genes' expression profile. Three different concentrations of Hcy were added in the culture medium of confluent monolayers. c RNAs were made from the isolated total RNAs and the labeled c RNA probes were hybridized to microarrays specific for human disease pathway inflammatory cytokines, chemokines and their receptor gene micro-array panels as per manufacture's recommendations. Two Hcy up-regulated molecules:IL6 and CEBPB were further validated via Western blot analysis. Hcy's effect on ARPE-19 cellular morphology and genomic DNA integrity were also evaluated.RESULTS:Gene microarray analyses of RPE cells in response to Hcy treatment revealed alterations in the expressions of several inflammatory gene transcripts such as CCL5, CEBPB, IL13RA2, IL15 RA, IL6, IL8 and CXCL3 that were up-regulated. The transcripts for C3, CCL2, IL11 RA and IL18 genes exhibited down-regulation. The IL6 and CEBPB expressions were subsequently validated at the protein levels. Treatment of the retinal cells with increasing Hcy concentration influenced their density in culture however their morphology and DNA integrity remained unaffected. CONCLUSION:These findings suggest that Hcy can potentially mediate the expression of chemokines,cytokines and interleukins receptors in the retinal cells without having any debilitating effects on their morphology and the genomic DNA integrity. | Mahavir Singh Suresh C Tyagi | 2017 | International Journal of Ophthalmology(English edition)2017，10，5： | 10 |
| 2 | A hypothesis for treating inflammation and oxidative stress with hydrogen sulfide during age-related macular degeneration显示文摘Age-related macular degeneration(AMD) is a leading cause of blindness and is becoming a global crisis since affected people will increase to 288 million by 2040.Genetics,age,diabetes,gender,obesity,hypertension,race,hyperopia,iris-color,smoking,sun-light and pyroptosis have varying roles in AMD,but oxidative stress-induced inflammation remains a significant driver of pathobiology.Eye is a unique organ as it contains a remarkable oxygengradient that generates reactive oxygen species(ROS) which upregulates inflammatory pathways.ROS becomes a source of functional and morphological impairments in retinal pigment epithelium(RPE),endothelial cells and retinal ganglion cells.Reports demonstrated that hydrogen sulfide(H_2S) acts as a signaling molecule and that it may treat ailments.Therefore,we propose a novel hypothesis that H_2S may restore homeostasis in the eyes thereby reducing damage caused by oxidative injury and inflammation.Since H_2S has been shown to be a powerful antioxidant because of its free-radicals' inhibition properties in addition to its beneficial effects in age-relatedconditions,therefore,patients may benefit from H_2S salubrious effects not only by minimizing their oxidant and inflammatory injuries to retina but also by lowering retinal glutamate excitotoxicity. | Akash K George Mahavir Singh Rubens Petit Homme Avisek Majumder Harpal S SANDhu Suresh C Tyagi | 2018 | International Journal of Ophthalmology(English edition)2018，11，5： | 3 |
| 3 | Genes and genetics in eye diseases: a genomic medicine approach for investigating hereditary and inflammatory ocular disorders显示文摘Past 25 y have witnessed an exponential increase in knowledge and understanding of ocular diseases and their respective genetic underpinnings. As a result, scientists have mapped many genes and their variants that can influence vision and health of our eyes. Based on these findings, it is becoming clear that an early diagnosis employing genetic testing can help evaluate patients' conditions for instituting treatment plan(s) and follow-up care to avoid vision complications later. For example, knowing family history becomes crucial for inherited eye diseases as it can benefit members in family who may have similar eye diseases or predispositions. Therefore, gathering information from an elaborate examination along with complete assessment of past medical illness by ophthalmologists followed by consultation with geneticists can help create a roadmap for making diagnosis and treatment precise and beneficial. In this review, we present an update on ocular genomic medicine that we believe has tremendous potential towards unraveling genetic implications in ocular diseases and patients' susceptibilities. We also discuss translational aspects of genetic ophthalmology and genome engineering that may help advance molecular diagnostics and therapeutics. | Mahavir Singh Suresh C Tyagi | 2018 | International Journal of Ophthalmology(English edition)2018，11，1： | 2 |
| 4 | Review on thermal energy storage with phase change materials and applications 显示文摘 | Sharma A Tyagi V V Chen C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，2： | 1 |
| 5 | Collagen network of the myocardium function,structural remodeling and regulatory mechanisms显示文摘 | Weber K T Sun Y Tyagi S C | 1994 | J Mol Cell Cardiol1994，26，3： | 1 |
| 6 | Silibinin down-regulates survivin protein and mRNA expression and causes caspases activation and apoptosis in human bladder transitional-cell papilloma RT4 cells显示文摘 | TYAGI A K AGARWAL C SINGH R P | 2003 | Biochem Biophys Res Comm2003，312，4： | 1 |
| 7 | Uric acid: a new look at an old risk marker for cardio vascular disease, met abolic syndrome, and type 2 diabetes mellitus: the u rate redox shutter显示文摘 | HAYDEN M R TYAGI S C | 2004 | Nutr Metab (Lond)2004，1，： | 1 |
| 8 | Susceptibility to tuberculo- sis: clues from studies with inbred and outbred New Zealand White rabbits显示文摘 | Dorman S E Hatem C L Tyagi S | 2004 | Infect Immun2004，72，3： | 1 |
| 9 | Mechanisms of endothelial dysfunction with development of type 1 diabetes mellitus role of insulin and C-peptide显示文摘 | Bratcher A P Rodriguez W E Tyagi S C | 2005 | Cell Biochem2005，96，6： | 1 |
| 10 | Endocrine disrupting compounds removal from wastewater, a new challenge 显示文摘 | Auriol M Filali-Meknassi Y Tyagi R D Adams C D Surampalli R Y | 2006 | Process Biochemistry2006，41，3： | 1 |
| 11 | Silibinin causes cellcycle arrest and apoptosis in human bladder transitional cellcarcinoma cells by regulating CDKI - CDK - cyclin cascade,and caspase 3 and PARP cleavages显示文摘 | Tyagi A Agarwal C Harrison G | 2004 | Carcinogenesis2004，25，9： | 1 |
| 12 | Bacillus licheniformis proteases as high value added products from fermentation ofwastewater sludge: pre-treatment of sludge to increase the performance of the process 显示文摘 | Drouin M Lai C K Tyagi R D | 2008 | Water Science and Technology2008，57，3： | 1 |
| 13 | Review on ther- mal energy storage with phase change materials and applications 显示文摘 | SHARMA A TYAGI V V CHEN C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，2： | 1 |
| 14 | Silibinin acti- vates p53-caspase 2 pathway and causes caspase-mediated cleavage of Cipl/p21 in apoptosis induction in bladder tran- sitional-cell papilloma RT4 cells: evidence for a regulatory loop between p53 and caspase 2显示文摘 | TYAGI A SINGH R P AGARWAL C | 2006 | Carcinogenesis2006，27，11： | 1 |
| 15 | Reliability,risk and uncertainty analysis using generic expection functions 显示文摘 | Tyagi A Haan C T | 2001 | Journal of Environmental Engineering2001，127，10： | 1 |
| 16 | Silibinin causes cell cycle arrest and apoptosis in human bladder tran- sitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade,and caspase 3 and PARP cleavages显示文摘 | TYAGI A AGARWAL C HARRISON G | 2004 | Carcinogene- sis2004，25，9： | 1 |
| 17 | Review on thermal energy storage with phase change materials and applications 显示文摘 | SHARMA A TYAGI V V CHEN C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，： | 1 |
| 18 | A Fuzzy Logic Based Load Frequency Controller In a Competitive Electricity Environment显示文摘 | TYAGI B SRIVASTAVA S C | 2003 | Power Engineering Society General Meeting IEEE July2003，，2： | 1 |
| 19 | Silibinin down-regulates survivin protein and mRNA expression and causes caspases activation and apoptosis in human bladder transitional-cell papilloma RT4 cells 显示文摘 | Tyagi AK Agarwal C Singh RP | 2003 | Biochem Biophys Res Commun2003，312，4： | 1 |
| 20 | Folio acid improves inner ear vascularization in hyperhomocysteinemic mice显示文摘 | Kundu S Munjal C Tyagi N | 2012 | Hear Res2012，284，12： | 1 |
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