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| 1 | Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes显示文摘Objective: The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new application for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3 T3-L1 preadipocytes/adipocytes as a model.Methods: Three leaf extracts and thirteen flavonoids isolated from C. odorata were tested for their effect on lipid accumulation in 3 T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive control. The effects of active flavonoids on the adipocytes were confirmed by oil red 0 staining and visualized under a light microscope.Results: n-Hexane and ethyl acetate extracts of C. odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 μg/ml. Four flavonoids, pectolinarigenin, kaempferide, 4,2'-dihydroxy-4',5',6'-trimethoxychalcone and dillenetin, exhibited significant, concentrationdependent inhibitory effects on lipid accumulation in 3 T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2'-dihydroxy-4',5',6'-trimethoxychalcone, which caused 75% and 90% inhibition of cellular lipid accumulation at 30 and 50 μmol/L, respectively. Both kaempferide and 4,2'-dihy droxy-4',5',6'-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant.Conclusion: C. odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accumulation in 3 T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids. | Supakanya Kumkarnjana Rutt Suttisri Ubonthip Nimmannit Thongchai Koobkokkruad Chutichot Pattamadilok Nontima Vardhanabhuti | 2018 | Journal of Integrative Medicine2018，16，6： | 2 |
| 2 | Preparation of turmeric oil-loaded chitosanalginate biopolymeric nanocapsules 显示文摘 | Pranee Lertsutthiwong Pornchai Rojsitthisak Ubonthip Nimmannit | 2009 | Materials Science and Engineering C2009，，29： | 1 |
| 3 | Preparation of tur- meric oil loaded chitosan-alginate biopolymeric nanocap- sules显示文摘 | Pranee L Pornchai R Ubonthip N | 2009 | Materials Science and Engineering C2009，29，： | 1 |
| 4 | Nitric oxide regulates cell sensitivity to cisplatin-induced apoptosis through s-nitrosylation and inhibition of bcl-2 ubiquitination显示文摘 | PITHI C UBONTHIP N CHRISTIAaN S | 2006 | Cancer Research2006，66，： | 1 |
| 5 | Preparation of turmeric oil-loaded chitosan-alginate biopolymeric nanocapsules显示文摘 | Pranee Lertsutthiwong Pornchai Rojsitthisak Ubonthip Nimmannit | | 0，，： | 1 |
| 6 | 显示文摘 | Pranee L Pornchai R Ubonthip N | 2009 | Mater Sci Eng C2009，29，： | 1 |
| 7 | Preparation of tnrmericoil-loaded chitosan-alginate biopolymerienanocapsules显示文摘 | PRANCE LERTSUTTHIWONG POMCHAIGO JSITTHISAK UBONTHIP NIMMANNIT | 2009 | Materials Science and Engineering C2009，，29： | 1 |
| 8 | Curcumin sensitizes non-small cell lung cancer cell anoikis through reactive oxygen species-mediated Bcl-2 downregulation显示文摘 | Varisa Pongrakhananon Ubonthip Nimmannit Sudjit Luanpitpong Yon Rojanasakul Pithi Chanvorachote | 2010 | Apoptosis2010，，5： | 1 |
| 9 | Flavonoids kaempferide and 4,2’-dihydroxy-4’,5’,6’-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells显示文摘Objective: Kaempferide and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone (DTMC) are two major flavonoids found in Chromolaena odoraia Linn, leaf extract. The aim of this study was to elucidate the mechanism by which these two flavonoids exerted their effect on adipogenesis. The inhibitory effect of kaempferide and DTMC on adipocyte differentiation and their mechanisms involving mitotic clonal expansion (MCE) and apoptosis during the early stage of adipogenesis were investigated. Methods: Confluent 3T3-L1 preadipocytes were induced to differentiate and exposed to the flavonoids during various phases of difTerentiaiion. Intracellular lipid accumulation, cell density and expression of the transcription factors peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding pro-teins a were assessed using AdipoRed, Oil red 0 and Western blot assays. Effects of both flavonoids on cell proliferation and apoptosis were also determined by carboxyfluorescein diacetate succinimidyl ester and annexin V-fluorescein isothiocyanate/propidium iodide-staining assays, respectively. Results: Kaempferide and DTMC showed significant, concentration-dependent anti-adipogenic activity and effect on cell density in the early phase of adipogenesis. The expression of the transcription factors seemed to be reduced when the treatment was prolonged or in the early phase of adipogenesis. These flavonoids interrupted MCE via inhibition of preadipocyte proliferation and induction of apoptosis. DTMC was nearly three times more potent than kaempferide in inducing apoptosis. Conclusion: Kaempferide and DTMC exerted their anti-adipogenic activity through inhibition of MCE, either by suppressing cell proliferation or by inducing apoptosis during the early phase of differentiation. | Supakanya Kumkarnjana Rutt Suttisri Ubonthip Nimmannit Apirada Sucontphunt Mattaka Khongkow Thongchai Koobkokkruad Nontima Vardhanabhuti | 2019 | Journal of Integrative Medicine2019，17，4： | 0 |
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