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| 1 | Cloning and sequence characteristics of the genomic gene of a rice metallothionein显示文摘Northern blot analysis showed that a metallothionein gene, ricMT is expressed strongly in the stem of rice with an expression level that could be more than 100-fold stronger than in leaf blades. The results suggest that the 5’ upstream region flanking the coding sequence of the ricMT may contain a fairly strong promoter To elucidatr its regulation and promoter structure, the genomic clones of ricMT were screened out from a rice genomic library and a fragment of about 4084 bp was sequenced. The fragment included a 5’ upstream region of ca. 2970 bp, a transcription region of ca. 690 bp and a 3’ downstream region of ca. 420 bp. Computer analysis of the sequence homology showed that the 5’ upstream region included a putative TATA box, a putative CAAT box, and a typical metal-responsive element TGCGCGCG. The results will promote further understanding of the mechanisms of gene regulation and metal response of plant metallothionein proteins. | YU Lihua, LIU Jinyuan, Masaaki Umeda, Hirofumi Uchimiya & ZHAO Nanming Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Beijing 100084, China Institute of Molecular and Cellular Bioscie | 2000 | Chinese Science Bulletin2000，45，2： | 4 |
| 2 | Application of real-time RT-PCR quantification to evaluate differential expression of Arabidopsis Aux/IAA genes显示文摘The molecular techniques including Northern blot, dot blot, in situ hybridization, etc. have been successfully used to estimate semi-quantitatively mRNA levels in plant samples. In this study, we employed a real-time reverse transcription-PCR (RT-PCR) assay using SYBR Green I fluorescence methodology to evaluate accurate quantitation and sequence specific detection of Aux/IAA mRNA levels in Arabidopsis. Results obtained indicate a linear dynamic range of 102-106 Aux/IAA mRNA copies with standard deviations of generally less than 15%. As a model experiment, the outcome of analysis of expression patterns of five Aux/IAA genes in Arabidopsis under various chemical and temperature treatments is presented. The method presented here provides a sensitive and rapid technique to evaluate plant Aux/IAA mRNA expression levels in nanogram order. | LIU Jinyuan Chiharu Oura Evalour T.Aspuria Yutaka Oono Hirofumi Uchimiya | 2001 | Chinese Science Bulletin2001，46，19： | 2 |
| 3 | Transgenic rice plants after direct gene trans~r protoplasts显示文摘 | Tofiyana K Afimoto Y Uchimiya H et ~ | 1988 | BioTechnology1988，6，： | 1 |
| 4 | In- fluence of Pyrolysis Temperature on Biochar Property and Function as a Heavy Metal Sorbent in Soil显示文摘 | Uchimiya M Wartelle L H Klasson K T | 2011 | Journal of Agricultural and Food Chemistry2011，59，6： | 1 |
| 5 | Molecular and physiological evaluation of transgenic tobacco plants expressing a maize phospho enol pyruvate carboxylase gene under the control of the cauliflower mosaic virus 35S promoter显示文摘 | Hiroyuki Kogami Mariko Shono Takayoshi Koike Syuuichi Yanagisawa Katsura Izui Naoki Sentoku Shigeyuki Tanifuji Hirofumi Uchimiya Seiichi Toki | 1994 | Transgenic Research1994，，5： | 1 |
| 6 | Ara6,a plantunique novel type Rab GTPase,functions in the endocytic pathway of Arabidopsis thaliana显示文摘 | YAMAGUCHI M UCHIMIYA H | 2001 | EMBO J2001，，20： | 1 |
| 7 | Suppression of thymidine phosphorylase-mediated angiogenesis and tumor growth by 2-deoxy-L-ribose显示文摘 | Uchimiya H Furukawa T Okamoto M | 2002 | Cancer Res2002，62，： | 1 |
| 8 | Influence of pyrolysis temperature on biochar property and function as a heavy metal sorbent in soil 显示文摘 | UCHIMIYA M WARTELLE L H KLASSON K T | 2011 | Journal of Agricultural and Food Chemistry2011，59，6： | 1 |
| 9 | 显示文摘 | E. Aspuria C. Ooura G. Chen H. Uchimiya Y. Oono | 2002 | Plant Cell Reports2002，，1： | 1 |
| 10 | Screening bio- chars for heavy metal retention in soil:Role of oxygen functional groups显示文摘 | Uchimiya M Chang S Klasson K T | 2011 | Journal of Hazardous Materi- als2011，190，13： | 1 |
| 11 | Isolation and expression analysis of two rice genes encoding the major intrinsic protein显示文摘 | Qiang Liu Masaaki Umeda Hirofumi Uchimiya | 1994 | Plant Molecular Biology1994，，6： | 1 |
| 12 | Immobilization of heavy metal ions by broiler litter-derived bioehars in water and soil显示文摘 | Uchimiya M Lima I M Klasson K T | 2010 | Joumal of Agrieultural and Food Chemistry2010，58，9： | 1 |
| 13 | Influence of soil prop- erties on heavy metal sequestration by biochar amendment:Cop- per sorption isotherms and the release of cations 显示文摘 | Uchimiya M Klasson KT Wartelle LH | 2011 | Chemo- sphere2011，82，10： | 1 |
| 14 | Trangsgenic rice plants after direct gene transfer into protoplasts显示文摘 | Toriyama K Arimoto Y Uchimiya H | 1988 | Bio/Technology1988，6，： | 1 |
| 15 | Bialaphos treatment of transgenic rice plants expressing a bar gene prevents infection by the sheath blight pathogen (Rhizoctonia solani)显示文摘 | Uchimiya H Iwata M Nojiri C | 1993 | Bio/Technology1993，11，： | 1 |
| 16 | Isolation and characterization of two cDNA clones for mRNAs that are abundantly expressed in immature anthers of rice (Oryza sativa L.)显示文摘 | Yukako Hihara Chikage Hara Hirofumi Uchimiya | 1996 | Plant Molecular Biology1996，，6： | 1 |
| 17 | Supression of thymidine phosphorylase-mediated angiogenesis and tumor growth by 2-deoxy-L-ribose 显示文摘 | Uchimiya H Furukawa T Okamoto M | 2002 | Cancer Res2002，62，10： | 1 |
| 18 | Immobilization of Heavy Metal Ions (Cu Ⅱ, Cd Ⅱ, Ni Ⅱ, and Pb Ⅱ )by Broiler Litter-Derived Biochars in Water and Soil 显示文摘 | Uchimiya M Lima I M Klasson K T | 2010 | Journal of Agricultural and Food Chemistry2010，58，9： | 1 |
| 19 | Overexpression of Bax inhibitor suppresses the fungal elicitor-induced cell death in rice (Oryza sativa L) cells显示文摘 | Matsumura H Nirasawa S Kiba A Urasaki N Saitoh H Ito M Kawai-Yamada M Uchimiya H Terauchi R | 2003 | Plant J2003，33，3： | 1 |
| 20 | Influence of pyrolysistemperature on biochar property and function as a heavy metal sorbentin soil显示文摘 | Uchimiya M Wartelle L H KIasson K T | 2011 | Journal of Agricultural and Food Chemistry2011，59，6： | 1 |
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