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| 1 | Geochemical Stratigraphy and Microvertebrate Assemblage Sequences across the Silurian/Devonian Transition in South China显示文摘跨越志留纪 / 泥盆纪转变的非海洋的碎屑状的岩石和浅海的碳酸盐和黑页岩的碳同位素(13Corg ) 分析在东方云南的 Qujing 和四川的 Zoige 从二个丰富地含化石的序列被比较,华南。在华南的二节, Xishancun 和 Putonggou 节,表明积极 13Corg 变在上面的 Pridoli 和更低的泥盆纪发生并且到达象25.2%一样重的山峰价值( Xishancun )并且19.9%( Putonggou )在最低 Lochkovian 跟随 thelodont Parathelodus 和 conodont Icriodus woschmidti woschmidti 的第一出现(仅仅在 Putonggou 节并且和 Protathyris-Lanceomyonia 腕足类动物的一种动物志)。这些结果在 13Corg 复制全球性已知的积极移动从最高志留纪到最低泥盆纪。越过在在华南的二节的志留纪 / 泥盆纪边界(SDB ) 的 13Corg 变化在类似于详细 SDB 的同位素的作文从在布拉格盆在 Klonk 全球标准 Stratotype 节和点(GSSP ) 的顶钻的地上凿穿 Klonk-1 弄弯的碳展出移动,捷克的共和国。另外,包括 Liaojiaoshan, Xishancun, Yanglugou 和 Xiaputonggou 集合,四个 microvertebrate 集合从分别地在 Xishancun 和 Putonggou 节暴露的志留纪 / 泥盆纪转变被认出。从两碳同位素地层学和 microvertebrate 集合顺序的结果建议在华南的 SDB 在 Xishancun 形成的底被定位(在样品 QX-20 和样品 QX-21 之间)在 Xishancun 节和 Xiaputonggou 形成的更低的部分(在样品 ZP-09 和样品 ZP-10 之间)在里面 Putonggou 节。为和越过 SDB 的 microvertebrate 遗体的变化的器官的碳的同位素的趋势能从不同沉积外形把一条途径提供给 SDB 的潜在的关联,它帮助水兵到相互关联做好非海洋的存款。 | ZHAO Wenjin WANG Nianzhong ZHU Min Ulrich MANN Ulrich HERTEN Andreas LǖKE | 2011 | Acta Geologica Sinica(English Edition)2011，85，2： | 9 |
| 2 | 鹅细小病毒VP3基因重组禽痘病毒转移载体的构建显示文摘从含有鹅细小病毒 (GPV)H1株主要结构蛋白VP3基因的重组质粒pPROEXHTb VP3中切取GPVH 1株VP3基因片段 ,将其亚克隆于 pSY5 3 8的EcoRⅠ位点 ,并将带有痘苗病毒启动子P11的LacZ报告基因平端克隆于上述重组子的SmaⅠ位点 ,再用NotⅠ切下同时含有VP3基因和LacZ报告基因的片段 ,亚克隆于pSY681的NotⅠ位点 ,构建了含有VP3基因的重组禽痘病毒转移载体。 | 田丽红 贾永清 王君伟 李一经 赵丽荣 Ulrich Neu mann | 2002 | 中国兽医科技2002，32，9： | 5 |
| 3 | Expulsion of oil from petroleum source rocks: inferences from pyrolysis of samples of unconventional grain size显示文摘 | Namik Yalcin M Mann Ulrich | 1998 | Organic Geochemistry1998，29，13： | 1 |
| 4 | Variability of petroleum inclusions in vein,fossil and vug cements Ageochemical study in the Barrandian basin(Lower Palaeozoic, Czech Republic) 显示文摘 | Herbert Volk Brian Horsfield Ulrich Mann | 2002 | Organic Gcochemistry2002，33，12： | 1 |
| 5 | Filament-based smoke with vortex shedding and variational reconnection显示文摘 | Steffen Wei?mann Ulrich Pinkall | 2010 | ACM Transactions on Graphics (TOG)2010，，4： | 1 |
| 6 | Petrology, palynology and organic geochemistry of Eocene lignite of Matanomadh, Kutch Basin, western India: Implications to depositional environment and hydrocarbon source potential显示文摘 | Suryendu Dutta Runcie P. Mathews Bhagwan D. Singh Suryakant M. Tripathi Alpana Singh Pratul K. Saraswati Santanu Banerjee Ulrich Mann | 2010 | International Journal of Coal Geology2010，，1： | 1 |
| 7 | Synthesis of tertiary amyl methyl ether(TAME):Equilibrium of the Multiple Reactions显示文摘 | Carsten Oost Kai Sundmacher Ulrich Hof mann | | 0，，18： | 1 |
| 8 | Filament-based smoke with vortex shedding and variational reconnection显示文摘 | Steffen Wei?mann Ulrich Pinkall | 2010 | ACM Transactions on Graphics (TOG)2010，，4： | 1 |
| 9 | Modeling and simulation of the injection of urea-water-solution for automotive SCR De NOx-systems 显示文摘 | Felix Birkhold A Ulrich Meingast Peter Wasser- mann | 2007 | Applied Catalysis B: Environmental2007，，70： | 1 |
| 10 | Experimental and theoretical investigations of a spray dryer with simultaneous chemical reaction显示文摘 | CHRISTIAN HORST ULRICH HO!MANN | 2001 | Chemical Engineering Science2001，56，： | 1 |
| 11 | Homologous recombination mediates stable Fah gene integration and phenotypic correction in tyrosinaemia mouse-model显示文摘AIM To stably correct tyrosinaemia in proliferating livers of fumarylacetoacetate-hydrolase knockout(Fah-/-) mice by homologous-recombination-mediated targeted addition of the Fah gene.METHODS C57 BL/6 Fah?exon5 mice served as an animal model for human tyrosinaemia type 1 in our study. The vector was created by amplifying human Fah c DNA including the TTR promoter from a lentivirus plasmid as described. The Fah expression cassette was flanked by homologous arms(620 bp and 749 bp long) of the Rosa26 gene locus. Mice were injected with 2.1 × 108 VP of this vector(r AAV8-ROSA26.HAL-TTR.FahROSA26.HAR) via the tail vein. Mice in the control group were injected with 2.1 × 108 VP of a similar vector but missing the homologous arms(r AAV8-TTR.Fah). Primary hepatocytes from Fah-/-recipient mice, treated with our vectors, were isolated and 1 × 106 hepatocytes were transplanted into secondary Fah-/-recipient mice by injection into the spleen. Upon either vector application or hepatocyte transplantation NTBC treatment was stopped in recipient mice. RESULTS Here, we report successful HR-mediated genome editing by integration of a Fah gene expression cassette into the 'safe harbour locus' Rosa26 by recombinant AAV8. Both groups of mice showed long-term survival, weight gain and FAH positive clusters as determined by immunohistochemistry analysis of liver sections in the absence of NTBC treatment. In the group of C57 BL/6 Fah?exon5 mice, which have been transplanted with hepatocytes from a mouse injected with r AAV8-ROSA26.HAL-TTR.Fah-ROSA26.HAR 156 d before, 6 out of 6 mice showed long-term survival, weight gain and FAH positive clusters without need for NTBC treatment. In contrast only 1 out 5 mice, who received hepatocytes from r AAV8-TTR.Fah treated mice, survived and showed few and smaller FAH positive clusters. These results demonstrate that homologous recombinationmediated Fah gene transfer corrects the phenotype in a mouse model of human tyrosinaemia type 1(Fah-/-mice) and is long lasting in a proliferating state of the liver as shown by withdrawal of NTBC treatment and serial transplantation of isolated hepatocytes from primary Fah-/-recipient mice into secondary Fah-/-recipient mice. This long term therapeutic efficacy is clearly superior to our control mice treated with episomal r AAV8 gene therapy approach.CONCLUSION HR-mediated r AAV8 gene therapy provides targeted transgene integration and phenotypic correction in Fah-/-mice with superior long-term efficacy compared to episomal r AAV8 therapy in proliferating livers. | Norman Junge Qinggong Yuan Thu Huong Vu Simon Krooss Christien Bednarski Asha Balakrishnan Toni Cathomen Michael P Manns Ulrich Baumann Amar Deep Sharma Michael Ott | 2018 | World Journal of Hepatology2018，10，2： | 0 |
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