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| 1 | Controlling self-assembling and tumor cell-targeting of protein-only nanoparticles through modular protein engineering显示文摘Modular protein engineering is suited to recruit complex and multiple functionalities in single-chain polypeptides. Although still unexplored in a systematic way, it is anticipated that the positioning of functional domains would impact and refine these activities, including the ability to organize as supramolecular entities and to generate multifunctional protein materials. To explore this concept, we have repositioned functional segments in the modular protein T22-GFP-H6 and characterized the resulting alternative fusions. In T22-GFP-H6, the combination of T22 and H6 promotes selfassembling as regular nanoparticles and selective binding and internalization of this material in CXCR4-overexpressing tumor cells, making them appealing as vehicles for selective drug delivery. The results show that the pleiotropic activities are dramatically affected in module-swapped constructs, proving the need of a carboxy terminal positioning of H6 for protein self-assembling, and the accommodation of T22 at the amino terminus as a requisite for CXCR4^+ cell binding and internalization. Furthermore, the failure of self-assembling as regular oligomers reduces cellular penetrability of the fusions while keeping the specificity of the T22-CXCR4 interaction.All these data instruct how multifunctional nanoscale protein carriers can be designed for smart, protein-driven drug delivery, not only for the treatment of CXCR4^+ human neoplasias, but also for the development of anti-HIV drugs and other pathologies in which CXCR4 is a relevant homing marker. | Eric Volta-Duran Olivia Cano-Garrido Naroa Serna Hector Lopez-Laguna Laura Sanchez-Garcia Mireia Pesarrodona Alejandro Sanchez-Chardi Ramon Mangues Antonio Villaverde Esther Vazquez Ugutz Unzueta | 2020 | Science China Materials2020，63，1： | 2 |
| 2 | Pressure-con- trolled versus volume-controlled ventilation during one-lung ventilation for thoracic surgery显示文摘 | UNZUETA M C CASAS J I MORAL M V | 2007 | Anesth Analg2007，10，4： | 1 |
| 3 | Microstructure and wear resistance of pearlitic rail steels 显示文摘 | A J Perez- Unzueta J H Beynon | 1993 | Wear1993，，： | 1 |
| 4 | Pressure-controlled versus volume-controlled ventilation during one-lung ventilation for thoracic surgery显示文摘 | Unzueta MC Casas JI Moral MV | 2007 | Anesth Analg2007，104，5： | 1 |
| 5 | Semicontinuous emulsion copolymerization of methyl methacrylate and n-butyl acrylate ( 1 ) : Effect of mixed emulsifiers in seeded polymerization显示文摘 | UNZUETA E FORCADA J | 1995 | Polymer1995，36，5： | 1 |
| 6 | Alveolar recruitment improves ventilation during thoracic surgery: a randomized controlled trial显示文摘 | Unzueta C Tusman G Suarez-Sipmann F | 2012 | Br J Anaesth2012，108，3： | 1 |
| 7 | Intracellular targeting of CD44 + cells with self-assembling, protein only nanoparticles显示文摘 | Pesarrodona M Ferrer-Miralles N Unzueta U | 2014 | Int J Pharm2014，473，12： | 1 |
| 8 | Pressure-controlled versus volume-controlled ventilation during one-lung ventilation for thoracic surgery 显示文摘 | Unzueta M C Cases J I Moral M V | 2007 | Anesth Analg2007，104，5： | 1 |
| 9 | Alveolar recruitment improves ventilation during thoracic surgery: a randomized controlled trial 显示文摘 | Unzueta C Tusman G Suarez -Sipmann F | 2012 | Br J Anaesth2012，108，3： | 1 |
| 10 | Pressure- controlled versus volume-controlled ventilation during one- lung ventilation for thoracic surgery显示文摘 | UNZUETA M C CASAS J I MORAL M V | 2007 | Anesth Analg2007，104，5： | 1 |
| 11 | Microstructure and wear resistance of pearlitic rail steels 显示文摘 | A J Perez - Unzueta J H Beynon | 1993 | Wear1993，，： | 1 |
| 12 | Pressure-controlled versus Volume_controlled ventilation During One-lung Ventilation For Thoracic Surgery 显示文摘 | Carmen Unzueta M Ignaciocasas J Victoria Moral M | 2007 | International Anesthesia Research Society2007，104，： | 1 |
| 13 | Alveolar recruit- ment improves ventilation during thoracic surgery: a randomized controlled trial显示文摘 | Unzueta C Tusman G Suarez-Sipmann F | 2012 | Br J Anaesth2012，108，3： | 1 |
| 14 | Pressure-controlled versus vol-ume-controlled ventilation during one-lung ventilation for thoracic surgery显示文摘 | Unzueta MC CasasJI Moral MV | | 0，，： | 1 |
| 15 | Alveolar recruit- ment improves ventilation during thoracic surgery: a randomized controlled triM显示文摘 | Unzueta C Tusman G Suarez-Sipmann F | 2012 | Br J Anaesth2012，108，3： | 1 |
| 16 | Pressure controlled versus volume controlled ventilation during one-lung ventilation for thoracic surgery显示文摘 | Unzueta MC Casas JI Moral MV | | 0，，： | 1 |
| 17 | Prevalence and characteristics of three clinical phenotypes of chronic obstructive pulmonary disease (COPD)显示文摘 | José Luis Izquierdo-Alonso Jose Miguel Rodriguez-GonzálezMoro Pilar de Lucas-Ramos Irune Unzueta Xabier Ribera Esther Antón Antonio Martín | 2013 | Respiratory Medicine2013，，5： | 1 |
| 18 | Alveolar recruitment improves ventilation during thoracic surgery: a randomized controlled trial 显示文摘 | Unzueta C Tusman G Suarez-Sipmann F | 2012 | Br J Anaesth2012，108，3： | 1 |
| 19 | Perioperative nutritional support显示文摘 | Morán López JM Piedra León M García Unzueta MT | 2014 | Cir Esp2014，92，6： | 1 |
| 20 | Pressure-controlled versus volume-controlled ventilation during one-lung ventilation for thoracic surgery显示文摘 | Unzueta MC Casas JI Moral MV | 2007 | Anesth Analg2007，104，5： | 1 |
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