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| 1 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Dis- solution kinetics of enargite in dilute C1JCl-media 显示文摘 | Herreros O Quiroz R Hemandez M C Vinals J | 2002 | Hydrometallurgy2002，64，： | 1 |
| 3 | Nitrate paradigm does nat hold up for sugarcane 显示文摘 | Robinson N Brackin R Vinall K | 2011 | Plos One2011，6，： | 1 |
| 4 | Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens显示文摘 | Vinale F Marra R Scala F | 2006 | Letters in Applied Microbiology2006，13，： | 1 |
| 5 | Fetal echocardiography at 11+0 to 13+6 weeks using four-dimensional spatiotemporal image correlation telemedicine via an Internet link:a pilot study显示文摘 | Vinals F Ascenzo R Naveas R | | 0，，06： | 1 |
| 6 | Accuracy in the determination of the Kovats retention index mathematical dead time 显示文摘 | Guardino X Albaiges J Firpo G Rodriguez - Vinals R Gassiot M | 1976 | J Chromatogr1976，118，： | 1 |
| 7 | Soil conditioning of sand for EPB applications: A laboratory research显示文摘 | VINAl R OGGERI C PElLA D | 2008 | Tunneling and Underground Space Technology2008，23，3： | 1 |
| 8 | Metronomic chemotherapy: an antiangiogenic scheduling 显示文摘 | LAQUENTE B VINALS F GERMA J R | 2007 | Clinical & Translational Oncology2007，9，2： | 1 |
| 9 | Genistein combined polysaceharide enhances activity of docetaxel,bicalutamide and Src kinase inhibition in androgen-dependent and independent prostate cancer cell lines显示文摘 | Burich R A Holland W S Vinall R L | 2008 | BJU Int2008，102，10： | 1 |
| 10 | The anticancer agent prodigiosin induces p21WAF1 expression via transforming growth factor-beta receptor pathway 显示文摘 | Soto-Cerrato V Vinals F Lambert J R | 2007 | Biochem Pharmacol2007，74，9： | 1 |
| 11 | Fetal echocardiography at 11 +0 to 13 + 6 weeks using four-dimensional spatiotemporal image correlation telemedicine via an Internet link: A pilot study 显示文摘 | Vinals F Ascenzo R Naveas R | 2008 | Ultrasound Obstet Gyneco12008，31，6： | 1 |
| 12 | The fetal cerebellar vermis:anatomy and biometric assessment using volume contrast imaging in the C-plane(VCI-C)显示文摘 | Vinals F Munoz M Naveas R | 2005 | Ultrasound Obstet Gynecol2005，26，6： | 1 |
| 13 | Major Secondary Metabolites Produced by Two Commercial Trichoderma Strains Active against Different Phytopathogens显示文摘 | VINALE F MARRA R SCALA F | 2006 | Applied Microbiology2006，43，2： | 1 |
| 14 | Simple approach to prenatal diagnosis of transposition of the great arteries显示文摘 | Vinals F Ascenzo R Poblete P | 2006 | Ultrasound Obstet Gynecol2006，28，1： | 1 |
| 15 | Fusion of phos- pholipid vesicles induced by muscle glyceraldehyde-3- phosphate dehydrogenase in the absence of calcium 显示文摘 | Morero R D Vinals A L Bloj B | 1985 | Biochemistry1985，24，8： | 1 |
| 16 | The Fetal Cerebellar Vermis:Anatomy and Biometric Assessment Using Volume Contrast Imaging in the C-plane (VCI-C)显示文摘 | Vinals F Munoz M Naveas R | | 0，，06： | 1 |
| 17 | The fetal cerebellar vermis:anatomy and biometric assessment using volume contrast imaging in the C-plane (VCI-C)显示文摘 | Vinals F Munoz M Naveas R | 2005 | Ultrasound obstet Gynecol2005，26，6： | 1 |
| 18 | Prodigiosin induces the proapoptotic gene NAG-1 via glycogen synthase kinase-3βactivity in human breast cancer cells显示文摘 | SOTO-CERRATO V VINALS F LAMBERT J R | 2007 | Molecular Cancer Therapeutics2007，6，1： | 1 |
| 19 | Simple approach to prenatal diagnosis of transposition of the great arteries 显示文摘 | Vinals F Ascenzo R Poblete P | 2006 | Ultrasound Obstet Gynecol2006，28，： | 1 |
| 20 | Simple approach to prenatal diagnosis of transposition of the great arteries显示文摘 | Vinals F Ascenzo R Poblete P | 2006 | Ultrasound in Obstetrics & Gynecology2006，28，1： | 1 |
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