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| 1 | Linagliptin alleviates fatty liver disease in diabetic db/db mice显示文摘AIM To study the effects of linagliptin on the structural signs of non-alcoholic fatty liver disease(NAFLD) in db/db mice. METHODS Male diabetic db /db mice(BKS.Cg-Dock7m+/+Leprdb/J) aged 10 wk received the dipeptidyl peptidase 4(DPP4) inhibitor linagliptin(10 mg/kg) or saline as a placebo once per day by gavage for 8 wk. Intact db/db mice served as controls. Structural changes in the liver were analyzed from light and electron microscopic images of sections from intact, placebo-treated and linagliptin-treated animals. We estimated the changes in hepatocytes, sinusoidal cells, liver microvasculature and lymphatic roots. Hepatic staining for lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1) was assessed by immunohistochemistry. RESULTS In 18-wk-old diabetic mice, liver steatosis(predominantly microvesicular and mediovesicular steatosis) was accompanied by dilation of the roots of the lymphatic system, interlobular blood vessels and bile canaliculi. Compared to saline-treated mice, linagliptin-treated mice exhibited a reduction in the mean numeral densities of hepatocytes with lipid droplets(92.4% ± 1.7% vs 64.9% ± 5.8% per field of view, P = 0.0002) and a lower proportion of hepatocytes with a high density of lipid droplets(20.7% ± 3.6% vs 50.4% ± 3.1%, P = 0.0007). We observed heterogeneous hepatocytes and relatively preserved cell structures in the linagliptin group. Dilation of blood and lymphatic vessels, as well as ultrastructural changes in the hepatocyte endoplasmic reticulum and mitochondria, were alleviated by linagliptin treatment. In intact and placebo-treated mice, immunohistochemical staining for LYVE-1 was observed in the endothelial cells of interlobular lymphatic vessels and on the membranes of some endothelial sinusoidal cells. We observed an enlarged LYVE-1 reaction area in linagliptin-treated mice compared to intact and placebo-treated mice. The improvement in the structural parameters of the liver in linagliptin-treated mice was independent to changes in the plasma glucose levels. CONCLUSION The DPP4 inhibitor linagliptin alleviates liver steatosis and structural changes in the hepatic microvasculature and lymphatic roots in a model of NAFLD in diabetic db/db mice. | Svetlana V Michurina Irina Ju Ishenko Vadim V Klimontov Sergey A Archipov Natalia E Myakina Marina A Cherepanova Eugenii L Zavjalov Galina V Koncevaya Vladimir I Konenkov | 2016 | World Journal of Diabetes2016，7，19： | 2 |
| 2 | Axitinib versus sorafenib as first-line therapy in patients with metastatic renal-cell carcinoma: a randomised open-label phase 3 trial显示文摘 | Thomas E Hutson Vladimir Lesovoy Salman Al-Shukri Viktor P Stus Oleg N Lipatov Angel H Bair Brad Rosbrook Connie Chen Sinil Kim Nicholas J Vogelzang | 2013 | Lancet Oncology2013，，13： | 2 |
| 3 | Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients显示文摘AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ2 = 3.89, all patients; χ2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (χ2 = 4.71, gastric cancer; χ2 = 4.11, gastrointestinal cancer; P < 0.05). The correlation was observed to exist between the level of anti-TF IgG and the count of lymphocytes (r = 0.517, P < 0.01), as well as between the level of anti- Tn IgG and that of serum CA 19-9 (r = 0.481, P < 0.05). No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stage- and morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens. | Eugeniy P Smorodin Oleg A Kurtenkov Boris L Sergeyev Kristel E Kodar Valentin I Chuzmarov Vladimir P Afanasyev | 2008 | World Journal of Gastroenterology2008，14，27： | 2 |
| 4 | What is organizational knowledge?显示文摘 | Tsoukas H Vladimirou E | 2001 | Journal of Management Studies2001，38，7： | 1 |
| 5 | Control and anti control of chaos in an analog neural network with time delay 显示文摘 | Bondarenko Vladimir E | 2002 | Chaos Solitons and Fractals2002，13，1： | 1 |
| 6 | Lipopilic tartaric acid esters as enantioselective ionophores显示文摘 | Vladimir P Mice K Martin E | 1989 | Angew Chem Int Ed1989，28，： | 1 |
| 7 | Thermal processing of sewage sludge (II) 显示文摘 | LUCIE H JAROSLAV B VLADIMIR U THOMAS E | 2008 | Appl Therm Eng2008，28，16： | 1 |
| 8 | Robust H position control synthesis of an electro-hydraulic servo system 显示文摘 | VLADIMIR M ZELJKO S MARIO E | 2010 | ISA Transactions2010，49，4： | 1 |
| 9 | Pae X, a Second Pectin Acetylesterase of Erwinia chrysanthemi 3937显示文摘 | Vladimir E Shevchik Nicole HCP | 2003 | JOURNAL OF BACTERIOLOGY2003，185，10： | 1 |
| 10 | The Exopolygalacturonate Lyase PelW and the Oligogalacturonate Lyase Ogl, Two Cytoplasmic Enzymes of Pectin Catabolism in Erwinia chrysanthemi 3937显示文摘 | VLADIMIR E SHEVCHIK GUY CONDEMINE | 1999 | JOURNAL OF BACTERIOLOGY1999，181，13： | 1 |
| 11 | Measurement of the concentration ratio for <SUP>13</SUP>? and <SUP>12</SUP>? isotopes at atmospheric pressure by carbon dioxide absorption of diode laser radiation at ~2 μm显示文摘 | E S Mironchuk I V Nikolaev Vladimir N Ochkin S S Rodionova Maksim V Spiridonov Sergei N Tskhai | 2009 | Quantum Electronics2009，，4： | 1 |
| 12 | Comparison of Al-induced gene expression in sensitive and tolerant soybean cultivar显示文摘 | Vladimir E Winfriede W Renate M | 2003 | Journal of Experiment Botany2003，54，： | 1 |
| 13 | Protective role of nitric oxide in ischemia and reperfusion injury of the liver显示文摘 | Tsuyoshi Shimamura Yue Zhu Shimin Zhang Maeng Bong Jin Naoki Ishizaki Atsushi Urakami Eishi Totsuka Akihiro Kishida Randall Lee Vladimir Subbotin Hiroyuki Furukawa Thomas E Starzl Satoru Todo | 1999 | Journal of the American College of Surgeons1999，，1： | 1 |
| 14 | Long-term hypoxia increases endothelial nitric oxide syn-thase expression in the ovine fetal adrenal显示文摘 | Tshepo R Monau Vladimir E Vargas Natalie King | 2009 | Reproduc-tive Sciences2009，16，9： | 1 |
| 15 | Conformal anomaly as a source of soft pho-tons in heavy ion col isions显示文摘 | Gokce B Dmitri E K Vladimir S | | 0，，： | 1 |
| 16 | Cluster discovery techniques for exploratory spatial data analysis 显示文摘 | VLADIMIR E 1998 | 1998 | Geographical Information Science1998，12，5： | 1 |
| 17 | Cardiogenic shock complicating acute myocardial infarction—etiologies, management and outcome: a report from the SHOCK Trial Registry显示文摘 | Judith S Hochman Christopher E Buller Lynn A Sleeper Jean Boland Vladimir Dzavik Timothy A Sanborn Emilie Godfrey Harvey D White John Lim Thierry LeJemtel | 2000 | Journal of the American College of Cardiology2000，，3： | 1 |
| 18 | Fibromuscular dysplasia in an accessory renal artery causing renovaseular hypertension: a case report显示文摘 | Zeina AR Vladimir W Barmeir E | 2007 | J Med Case Reports2007，1，： | 1 |
| 19 | What is Organization Knowledge显示文摘 | Tsoukas H Vladimirou E | 2001 | Journal of Management Studies2001，38，7： | 1 |
| 20 | A (H1N1) pandemic influenza and its prevention by vaccination: paediatricians' opinions before and after the beginning of the vaccination campaign显示文摘 | Dube E Fannie D Vladimir G | 2011 | BMC Public Health2011，11，： | 1 |
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