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| 1 | Ectopic expression of clusterin/apolipoprotein J or Bcl-2 decreases the sensitivity of HaCaT cells to toxic effects of ropivacaine显示文摘Local anesthetics inhibit cell proliferation and induce apoptosis in various cell types. Ropivacaine, a unique, novel tertiary amine-type anesthetic, was shown to inhibit the proliferation of several cell types including keratinocytes. We found that Ropivacaine could inhibit the proliferation and induce apoptosis in an immortalized human keratinocyte line, HaCaT, in a dose- and time-dependent manner and with the deprivation of serum. The dose-dependent induction of apoptosis by ropivacaine was demonstrated by DNA fragmentation analysis and the proteolytic cleavage of a caspase-3 substrate — poly (ADP-ribose) polymerase (PARP). In addition, ropivacaine downregulated the expression of clusterin/ apoliporotein J, a protein with anti-apoptotic properties, in a dose-dependent manner, which well correlated with the induction of apoptosis of HaCaT cells. To investigate the role of clusterin/apoliporotein J in ropivacaine-induced apoptosis, HaCaT cells overexpressing clusterin/apoliporotein J were generated and compared to cells expressing the well estab- lished anti-apoptotic Bcl-2 protein. Ectopic overexpression of the secreted form of clusterin/apoliporotein J or Bcl-2 decreased the sensitivity of HaCaT cells to toxic effects of ropivacaine as demonstrated by DNA fragmentation, the proteolytic cleavage of PARP and by a reduction in procaspase-3 expression. Furthermore, the downregulation of endogenous clusterin/apolipoprotein J levels by ropivacaine suggested that this might be one mechanism by which ropivacaine induced cell death in HaCaT cells. In conclusion, the ability of ropivacaine to induce antiproliferative responses and to suppress the expression of the anti-apoptotic protein clusterin/apolipoprotein J, combined with previ- ously reported anti-inflammatory activity and analgesic property of the drug, suggests that ropivacaine may have poten- tial utility in the local treatment of tumors. | Evangelos KONTARGIRIS Evangelos KOLETTAS Athina VADALOUCA Ioannis P. TROUGAKOS Efstathios S. GONOS Vasiliki KALFAKAKOU | 2004 | Cell Research2004，14，5： | 2 |
| 2 | A comparative study of propofol and thiopental as induction agents for elective caesarean section 显示文摘 | Siafaka I Vadalouca A Gatziou B | 1992 | Clin Exp Obstet Gynecol1992，19，2： | 1 |
| 3 | A randomized,double-blind,placebo-controlled study of preemptively administered intravenous parecoxib:effect on anxiety levels and procedural pain during epidural catheter placement for surgical operations or for chronic pain therapy显示文摘 | Vadalouca A Moka E Chatzjdimitriou A | 2009 | Pain Pract2009，9，3： | 1 |
| 4 | Renin-aldosterone system alterations during abdominal gynaecological operations under general or combined general and epidural anaesthesia显示文摘 | Petropoulos G Vadalouca A Siafaka I | 2000 | Clin Exp Obstet Guynecol2000，27，1： | 1 |
| 5 | Opioid rotation in patients with cancer: a review of the current literature显示文摘 | VADALOUCA A MOKA E ARGYRA E SIKIOTI P SIAFAKA I | 2008 | J Opioid Manag2008，4，4： | 1 |
| 6 | Opioid rotation in patients with cancer:a review of the current literature显示文摘 | Vadalouca A Moka E Argyra E | | 0，，04： | 1 |
| 7 | Opioid rotation in patients with cancer:a review of the current literature显示文摘 | Vadalouca A Moka E A rgyra E | 2008 | J Opioid Manag2008，4，4： | 1 |
| 8 | A randomized ,double - blind, placebo - controlled study of preemptively administered intravenous parecoxib:effect on anxiety levels and procedural pain during epidural catheter placement for surgical operations or for chronic pain therapy 显示文摘 | Vadalouca A Moka E Chatzidimitriou A | 2009 | Pain Pract2009，9，3： | 1 |
| 9 | Pharmacological treatment of neuropathic cancer pain:a comprehensive review of the current literature显示文摘 | Vadalouca A Raptis E Moka E | 2012 | Pain Pract2012，12，3： | 1 |
| 10 | Opioid rotation in patients with cancer: a review of the current literature显示文摘 | Vadalouca A Moka E Argyra E | 2008 | J Opioid Manag2008，4，4： | 1 |
| 11 | Effect on anxiety levels and procedural pain during epidural catheter placement for surgical operations or for chronic pain therapy显示文摘 | Vadalouca A Moka E Chatzidimitriou A | 2009 | Pain Pract2009，9，3： | 1 |
| 12 | A random-ized,double-blind,placebo-controlled study of preemptively administered intravenous parecoxib:effect on anxiety levels and procedural pain during epidural catheter placement for surgical operations or for chronic pain therapy显示文摘 | Vadalouca A Moka E Chatzidimitriou A | 2009 | Pain Pract2009，9，3： | 1 |
| 13 | Pharmacological treatment of neuropathic cancer pain: acom prehensive review of the current literature 显示文摘 | Vadalouca A Raptis E Moka E | 2012 | Pain Pract2012，12，3： | 1 |
| 14 | A randomized,doubleblind,placebo-controlled study of preemptively administered intravenous parecoxib:effect on anxiety levels and procedural pain during epidural catheter placement for surgical operations or for chronic pain therapy显示文摘 | Vadalouca A Moka E Chatzidimitriou A | | 0，，03： | 1 |
| 15 | Effect on anxiety levels and procedural pain during epidural catheter placement for surgical operations or for chronic pain therapy 显示文摘 | Vadalouca A Moka E Chatzidimitriou A | 2009 | Pain Pract2009，9，3： | 1 |
| 16 | Opioid rotcation in patients with cancer:a review of the current literature显示文摘 | Vadalouca A Moka E Arqyra t | 2008 | J Opioid Manag2008，4，4： | 1 |
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