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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Droplet bar- coding for single-cell transcriptomics applied to embryonic stem cells显示文摘 | Klein AM Mazutis L Akartuna I Tallapragada N Veres A Li V Peshkin L Weitz DA Kirschner MW | 2015 | Ceil2015，161，： | 1 |
| 3 | Endoscopical and histological features in bile reflux gastritis显示文摘 | Vere CC Cazacu S Comanescu V | 2005 | Rom Morphol Embryol2005，46，4： | 1 |
| 4 | Design and control tradeoffs for rockbins in passively solar heated houses with trombe walls, direct gain and high solar fractious 显示文摘 | Sebald A V Vered G | 1987 | Solar Energy1987，39，4： | 1 |
| 5 | Endoscopical and histological features in bile reflux gastritis 显示文摘 | Vere CC Cazacu S Comanescu V | 2005 | Rom Morphol Em- bryol2005，46，4： | 1 |
| 6 | Endoscopical and histologi- cal features in bile reflux gastritis 显示文摘 | Vere CC Cazacu S Comanescu V | 2005 | Rom JMorphol Embryol2005，46，4： | 1 |
| 7 | Psychological dis-tress and social support are determinants of changing oral health status among an immigrant population from Ethio-pia显示文摘 | Vered Y Soskolne V Zini A | | 0，，02： | 1 |
| 8 | Development and e- valuation of pH-sensitive sodium alginate/chitosan microp articles containing the antituberculosis drug rifampicin显示文摘 | Lacerda L Parize A L Vere V | 2014 | MaterSci and Eng: C2014，39，： | 1 |
| 9 | Global optimization-based control algorithms applied to boundary layer transition problems显示文摘 | VERES G V TUTTY O R | 2004 | Control Engineering Practice2004，12，4： | 1 |
| 10 | Endoscopical and histological features in bile reflux gastritis显示文摘 | Vere CC Cazacu S Comanescu V | 2005 | Rom J Morphol Embryol2005，46，4： | 1 |
| 11 | Design and control tradeoffs for rockbins in passively solar heated houses with trombe walls, direct gain and high solar fractions 显示文摘 | Sebald A V Vered G | 1987 | Solar Energy1987，，4： | 1 |
| 12 | Endoscopical and histological features in bile refluxgastritis 显示文摘 | VERE CC CAZACU S COMANESCU V | 2005 | Rom Morphol Embryo12005，46，4： | 1 |
| 13 | Effect of crosslingking agents on chitosan microspheres in controlled release of diclofenac sodium显示文摘 | Goncalves V L Laranjeira M Fávere V T | 2005 | Polimeros2005，15，1： | 1 |
| 14 | The interaction between reaetive dye containing vinylsulfone group and ehitosan microspheres 显示文摘 | KIMURA I Y LARANJEIRA M C DE F VERE V T | 2002 | International Journal of Polymeric Materials2002，51，8： | 1 |
| 15 | Endoscopical and histologi- cal features in bile reflux gastritis 显示文摘 | Vere CC Cazacu S Comainescu V | 2005 | Rom J Morphol Embryol2005，46，4： | 1 |
| 16 | Endoscopical and histological features in bile reflux gastritis显示文摘 | Vere CC Cazacu S Comnescu V | 2005 | Rom J Morphol Embryol2005，46，4： | 1 |
| 17 | Cross-linked quaternary chitosan as an adsorbent for the removal ofthe reactive dye from aqueous solutions显示文摘 | Rosa S Laranjeira M C M Riela H G Fávere V T | 2008 | Journal of Hazard-ous Materials2008，155，12： | 1 |
| 18 | Simultaneous photoluminescence and SERS observation of nanodiamond at laser deposition on noble metals 显示文摘 | Karmenyan A V Perevedentseva E Veres M | 2013 | Plasmonics2013，8，2： | 1 |
| 19 | Carotenoid composition and photochemical activity of four sandy grassland species 显示文摘 | Veres S Toth V R Laposi R | 2006 | Photosynthetica2006，44，2： | 1 |
| 20 | Effects of the available nitrogen on the photosynthetic activity and xanthophylls cycle pool of maize in field显示文摘 | Toth V R Meszkaros I Veres S at al | 2002 | Plant Physiol2002，159，： | 1 |
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