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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | The veres adapter: clinical experience with a new device for jet bronchoscopy ventilation via a laryngeal mask airway during flexible显示文摘 | Veres J Slavei K Errhalt P | 2011 | Anesth Analg2011，112，3： | 1 |
| 3 | Comparative study in the Ames test of benzo pyrene and 2-aminoanthra- cene metabolic activation using rat hepatic $9 and hep- atocytes following in vivo or in vitro induction 显示文摘 | Jenmitz K Veres Z Torok G | 2004 | Mu- tagenesis2004，19，3： | 1 |
| 4 | In vitro anti-diabetic activity and chemical characterization of an apolar fraction of Morus alba leaf water extract 显示文摘 | Hunyadi A Veres K Danko B | 2013 | Phytother Res2013，27，6： | 1 |
| 5 | TH1/TH2 imbalance,measured by circulating and intracytoplasmic inflammatory cytokines-immunological alterations in acute coronary syndrome and stable coronary artery disease显示文摘 | Szodoray P Timar O Veres K | 2006 | Scand J Immunol2006，64，3： | 1 |
| 6 | Electrical contact of redox enzymes with electrodes: novel approaches for amperometric biosensors显示文摘 | Eugenii K Vered H S Bilha W | 1997 | Bioelectrochem and Bioener1997，42，1： | 1 |
| 7 | TH1/TH2 imbal- ance,measured by circulating and intracytoplasmic in- flammatory cytokines--immunological alterations in acute coronary syndrome and stable coronary artery disease 显示文摘 | Szodoray P Timar O Veres K | 2006 | Scand J Immunol2006，64，3： | 1 |
| 8 | Biliary efflux transporters in volved in the clearance of rosuvastatin in sandwich culture of prima- ry rat hepatocytes显示文摘 | Jernnitz K Veres Z Tuqyi R | 2010 | Toxicol In Vitro2010，24，2： | 1 |
| 9 | Study of the oleanolic and ursolic acid contents of some species of the Lamiaceae显示文摘 | JANICSáK G VERES K KAKASY A Z | 2006 | Biochemical Systematics and Ecology2006，34，： | 1 |
| 10 | Th1/Th2 imbalance,measured by circulating and intracytoplasmic inflammatory cytokines immunological alterations in acute coronary syndrome and stable coronary artery disease显示文摘 | Szodoray P Timar O Veres K | 2006 | Scand J Immunol2006，64，3： | 1 |
| 11 | The neuropeptide calci- tonin gene-related peptide affects allergic airway inflammation by modulating dendritic cell function 显示文摘 | Rochlitzer S Veres TZ Kuhne K | 2011 | Clin Exp Allergy2011，41，11： | 1 |
| 12 | TH1/TH2 imbalance,measured by circulating and intracytoplasmic inflammatory cytokines-immunological alterations in acute coronary syndrome and stable coronary artery disease显示文摘 | Timar O Veres K | 2006 | Scand J Immunol2006，64，： | 1 |
| 13 | Early experiences with image-guided transoral surgery for the pathologies of the upper cervical spine显示文摘 | Veres R Bagó A Fedorcsák I | 2001 | Spine2001，26，12： | 1 |
| 14 | The neuropeptide ealcitonin gene-related peptide affects allergic airway inflammation by modulating dendritic cell function显示文摘 | Rochlitzer S Veres TZ Kiihne K | 2011 | Clin Exp Allergy2011，41，11： | 1 |
| 15 | Evaluation of peri-operative epidural analgesia with ropivacaine, ropivacaine andsufentanil, and ropivacaine, sufentanil and epinephrine in isofluraneanesthetized dogs undergoing tibial plateau levelling osteotomy 显示文摘 | Adami C Veres - Ny^ki K Spadavecchia C | 2012 | Veterin J2012，2，： | 1 |
| 16 | The neuropeptide calcitonin gene-related peptide affects allergic airway inflam- marion by modulating dendritic cell function 显示文摘 | Rochlitzer S Veres T Z Kiihne K | 2011 | Clin Exp Allergy2011，41，1： | 1 |
| 17 | Eevaluation of peri -operdural analgesia with ropivacaine, ropivacaine and sufen- tanil, and ropivacaine, sufentanil and epinephrine in isoflurane an- esthetized dogs undergoing tibial plateau leveling osteotomy 显示文摘 | Adami C Veres - Nyeki K Spadavecchia C | 2012 | Vet J2012，194，2： | 1 |
| 18 | Susceptibility to Klebsiella pneumonaie infection in collaborative cross mice is a complex trait controlled by at least three loci acting at different time points显示文摘 | Vered K Durrant C Mott R | 2014 | BMC Genomies2014，15，： | 1 |
| 19 | TH1/TH2 imbalance, measured by circulating and intracytoplasmic inflammatory cytokines-immunological alterations in acute coronary syndrome and stable coronary artery disease 显示文摘 | Szodoray P Timar O Veres K | 2006 | Scand J Immunol2006，64，3： | 1 |
| 20 | Evaluation of antibodies to oxidized low-density lipoprotein and assess ment of C-reactive protein in acute coronary syndrome and stable coronary artery disease显示文摘 | Soltesz P Veres K Laczik R | | 0，，02： | 1 |
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