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| 1 | Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium显示文摘Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey's fibers of the periodontal ligament (PDL). However, little is known about the regulatory morphogens that control differentiation and function of these progenitor cells, as well as the progenitor cells involved in crown and root formation. We investigated the role of bone morphogenetic protein-2 (Bmp2) in these processes by the conditional removal of the Bmp2 gene using the Sp7-Cre-EGFP mouse model. Sp7-Cre-EGFP first becomes active at E18 in the first molar, with robust Cre activity at postnatal day 0 (P0), followed by Cre activity in the second molar, which occurs after P0. There is robust Cre activity in the periodontium and third molars by 2 weeks of age. When the Bmp2 gene is removed from Sp7+ (Osterix+) cells, major defects are noted in root, cellular cementum and periodontium formation. First, there are major cell autonomous defects in root-odontoblast terminal differentiation. Second, there are major alterations in formation of the PDLs and cellular cementum, correlated with decreased nuclear factor IC (Nfic), periostin and α-SMA+ cells. Third, there is a failure to produce vascular endothelial growth factor A (VEGF-A) in the periodontium and the pulp leading to decreased formation of the microvascular and associated candidate stem cells in the Bmp2-cKOSp7-Cre-EGFP . Fourth, ameloblast function and enamel formation are indirectly altered in the Bmp2-cKOSp7-Cre-EGFP . These data demonstrate that the Bmp2 gene has complex roles in postnatal tooth development and periodontium formation. | Audrey Rakian Wu-Chen Yang Jelica Gluhak-Heinrich Yong Cui Marie A Harris Demitri Villarreal Jerry Q Feng Mary MacDougall Stephen E Harris | 2013 | International Journal of Oral Science2013，5，2： | 14 |
| 2 | In vitro antifungal activity of extracts of Mexican Chihuahuan Desert plants against postharvest fruit fungi显示文摘 | D. Jasso de Rodríguez R. Rodríguez García F.D. Hernández Castillo C.N. Aguilar González A. Sáenz Galindo J.A. Villarreal Quintanilla L.E. Moreno Zuccolotto | 2011 | Industrial Crops & Products2011，，1： | 2 |
| 3 | Surface topography and free energy regulate osteogenesis of stem cells: effects of shape-controlled gold nanoparticles显示文摘The surface free energy of a biomaterial plays an important role in the early stages of cell-biomaterial interactions,profoundly influencing protein adsorption,interfacial water accessibility,and cell attachment on the biomaterial surface.Although multiple approaches have been developed to engineer the surface free energy of biomaterials,systematically tuning their surface free energy without altering other physicochemical properties remains challenging.In this study,we constructed an array of chemically-equivalent surfaces with comparable apparent roughness through assembly of gold nanoparticles adopting various geometrically-distinct shapes but all capped with the same surface ligand,(1-hexadecyl)trimethylammonium chloride,on cell culture substrates.We found that bone marrow stem cells exhibited distinct osteogenic differentiation behaviours when interacting with different types of substrates comprising shape-controlled gold nanoparticles.Our results reveal that bone marrow stem cells are capable of sensing differences in the nanoscale topographical features,which underscores the role of the surface free energy of nanostructured biomaterials in regulating cell responses.The study was approved by Institutional Animal Care and Use Committee,School of Medicine,University of South Carolina. | Kamolrat Metavarayuth Esteban Villarreal Hui Wang Qian Wang | 2021 | Biomaterials Translational2021，2，2： | 2 |
| 4 | Progressive age -related changes in pulmonary tuberculosis images and the effect of diabetes显示文摘 | Perea GC Torres CS Villarreal VH | 2000 | Am J Res Crit Med2000，62，5： | 1 |
| 5 | Diagnostic approaches for diabetic cardiomyopathy and myocardial fibrosis显示文摘 | Maya L Villarreal FJ | 2010 | J Mol Cell Cardiol2010，48，3： | 1 |
| 6 | Screening metris of the Lang Ⅱ,Frisby,Randot,Titmus,and TNO stereo tests显示文摘 | Ohlsson J Villarreal G Abrahamsson M | 2001 | JAAPOS2001，5，5： | 1 |
| 7 | Tumor necrosis factor-alpha upregulates angiotensin Ⅱ type 1 receptors on cardiac fibroblasts 显示文摘 | Gurantz D Cowling RT Villarreal FJ | 1999 | Circulation Research1999，85，3： | 1 |
| 8 | The AP2 domain of AP2/EREBP define a large new family of DNA binding Protein in Arabidopsis显示文摘 | Okamuro K Caster B Villarreal R | 1997 | Proc Narl Acad Sci USA1997，94，13： | 1 |
| 9 | Intraarticular gene transfer of TNFR : Fc suppressed expettinental arthritis with reduced systemic distribution of the gene product显示文摘 | CHAN J M K VILLARREAL G JIN W W | 2002 | Mol Ther2002，6，6： | 1 |
| 10 | Response of a sedum green-roof to individual rain events 显示文摘 | Villarreal E L Bengtsson L | 2005 | Ecological En- gineering2005，25，1： | 1 |
| 11 | Internal carotid artery stenting for blunt carotid artery injuries with an associated pseudoaneurysm显示文摘 | Berne JD Reuland KR Villarreal DH | | 0，，2： | 1 |
| 12 | Mandibular condyle fractures: Determinants of treatment and outcome 显示文摘 | Villarreal PM Monje F Junquera LM | 2004 | J Oral Maxillofac Surg2004，62，2： | 1 |
| 13 | Cytotoxic activity of moronic acid and identification of the new triterpene 3,4-seco-olean-18-ene-3,28-dioic acid from Phoradendron reichenbachianum显示文摘 | RIOS M Y SALINA D VILLARREAL M L | 2001 | Planta Med2001，67，5： | 1 |
| 14 | Intraarticular gene transfer of TNFR: Fc suppresses experimental arthritis with reduced systemic distribution of the gene product显示文摘 | Chan J Villarreal G Jin W | 2002 | Mol Ther2002，6，6： | 1 |
| 15 | Coordinated regula-tion of extracellular matrix synthesis by the microRNA-29family in the trabecular meshwork显示文摘 | Villarreal G Jr Oh DJ Kang MH | 2011 | Invest Ophthalmol Vis Sci2011，52，6： | 1 |
| 16 | The pathogenesis of myocardial fibrosis in the setting of diabetic cardiomyopathy 显示文摘 | Asbun J Villarreal FJ | 2006 | J Am Coll Cardi ol2006，47，4： | 1 |
| 17 | The pathogenesis of myocardial fi- brosis in the setting of diabetic cardiomyopathy显示文摘 | Asbun J Villarreal F J | 2006 | J Am Coll Cardiol2006，47，4： | 1 |
| 18 | Differential MMP-2 ac- tivity of ligament cells under mechanical stretch injury: an in vitro study on human ACL and MCL fibroblasts显示文摘 | Zhou D Lee H S Villarreal F | 2005 | J Orthop Res2005，23，： | 1 |
| 19 | Aldosterone and antialdosterone therapy in congestive heart failure显示文摘 | WEBER K T VILLARREAL D | 1993 | Am J Cardiol1993，71，3： | 1 |
| 20 | Tumor necrosis factoralpha upregulates angiotensin Ⅱ type Ⅰ receptors on cardiac fibroblasts显示文摘 | Gurantz D Cowling RT Villarreal FJ | 1999 | Circulation Research1999，85，3： | 1 |
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