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| 1 | In vitro culture and differentiation of rat embryonic midbrain-derived neural stem cells显示文摘BACKGROUND:Midbrain-derived neural stem cells (mNSCs) can differentiate into functional mature dopaminergic neurons. The mNSCs are considered the ideal choice for cell therapy of Parkinson’s disease. OBJECTIVE: To isolate rat embryonic mNSCs and to observe the differentiation characteristics of mNSCs induced by cell growth-promoting factors. DESIGN,TIME AND SETTING: An in vitro cell culture study based on the molecular biology of nerve cells was carried out at the Institute of Clinical Medicine,China-Japan Friendship Hospital (China) from March to November 2007. MATERIALS: Sprague Dawley rats at embryonic day 14 were used in this study. Nestin antibody,β-Ⅲ tubulin antibody,glial fibrillary acidic protein (GFAP) antibody and cyclic nucleotide 3’-phosphohydrolase (CNPase) antibody were provided by Abcam; DMEM/F12 medium and N2 supplement were provided by In-vitrogen; epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF2) were provided by R&D Sys-tems. METHODS: The ventral mesencephalon was dissected from embryonic day 14 rat embryos. By trypsin di-gestion and mechanical separation,the brain tissue was triturated into a fine single-cell suspension. The cells were cultured in 5 mL serum-free medium containing DMEM/F12,1% N2 supplement,20 ng/mL EGF and FGF2. The mNSCs at the third generation were coated with 10 μg/mL polylysine and induced to differenti-ate in the DMEM/F12 supplemented with 1% fetal bovine serum and 1% N2. MAIN OUTCOME MEASURES: The neural spheres of the third passage were identified by nestin im-munofluorescence; at the same time,the cells were induced to differentiate,and the types of differentiated cell were identified by immunofluorescence for βⅢ tubulin,GFAP and CNPase. RESULTS: Seven days after primary culture,a great many neurospheres could be obtained by successive pasage. Immunofluorescence assays showed that the neurospheres were nestin positive,and after differentia-tion,the cells expressed GFAP,CNPase and β-Ⅲ-tubulin. CONCLUSION: Embryonic day 14 rat mNSCs can differentiate into neuron-like cells and glial cells fol-lowing induction by EGF,FGF2 and N2 additive. | Xingli Deng Ruen Liu Zhongtang Feng Jing Guo Wu Wang Deqiang Lei Hongyan Li Zhihua Chen | 2008 | Neural Regeneration Research2008，3，11： | 19 |
| 2 | Direct observation of ultrafast plasmonic hot electron transfer in the strong coupling regime显示文摘Achieving strong coupling between plasmonic oscillators can significantly modulate their intrinsic optical properties.Here,we report the direct observation of ultrafast plasmonic hot electron transfer from an Au grating array to an MoS_(2) monolayer in the strong coupling regime between localized surface plasmons(LSPs)and surface plasmon polaritons(SPPs).By means of femtosecond pump-probe spectroscopy,the measured hot electron transfer time is approximately 40 fs with a maximum external quantum yield of 1.65%.Our results suggest that strong coupling between LSPs and SPPs has synergetic effects on the generation of plasmonic hot carriers,where SPPs with a unique nonradiative feature can act as an‘energy recycle bin’to reuse the radiative energy of LSPs and contribute to hot carrier generation.Coherent energy exchange between plasmonic modes in the strong coupling regime can further enhance the vertical electric field and promote the transfer of hot electrons between the Au grating and the MoS_(2) monolayer.Our proposed plasmonic strong coupling configuration overcomes the challenge associated with utilizing hot carriers and is instructive in terms of improving the performance of plasmonic opto-electronic devices. | Hangyong Shan Ying Yu Xingli Wang Yang Luo Shuai Zu Bowen Du Tianyang Han Bowen Li Yu Li Jiarui Wu Feng Lin Kebin Shi Beng Kang Tay Zheng Liu Xing Zhu Zheyu Fang | 2019 | Light(Science & Applications)2019，8，1： | 7 |
| 3 | Attenuated phenotypes and analysis of a herpes simplex virus 1strain with partial deletion of the UL7, UL41 and LAT genes显示文摘We previously constructed a herpes simplex virus 1(HSV-1) UL7 mutant virus(M1) and showed that a partial deletion mutation of the UL7 gene led to a lower proliferative rate and an attenuated phenotype. Using the M1 mutant, we further modified the UL41 gene, which encodes another tegument protein, and the latency-associated transcript(LAT) gene. Observations of the resulting mutants with modified UL7 and UL41(M2) or UL7, UL41 and LAT(M3) genes indicated attenuated phenotypes, with lower proliferative ratios in various cells, non-lethal infections in mice and lower viral loads in nervous tissues compared with the wild-type strain. Furthermore, no LAT stable intron could be detected in the trigeminal ganglion of M3-infected animals. The results obtained with the three HSV-1 mutants indicate that the M3 mutant is an attenuated strain with low pathogenicity during both acute and latent infections. Together, the results support the use of the M3 mutant as a candidate for the development of an HSV-1 vaccine. | Xingli Xu Yingqiu Guo Shengtao Fan Pingfang Cui Min Feng Lichun Wang Ying Zhang Yun Liao Xiaolong Zhang Qihan Li | 2017 | Virologica Sinica2017，32，5： | 6 |
| 4 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 5 | TECHNOLOGY OF QUANTITATIVE ANALYSIS ON VIBRATORY STRESS RELIEF BASED ON ULTRASONIC TIME-OF-ARRIVAL METHOD显示文摘The effect of vibratory stress relief (VSR) is usually evaluated with the indirect method of ob-serving the change of amplitude frequency response characteristics of structures. A new kind of evaluatingmethod of VSR based on the ultrasonic time-of-arrival method (UTM), which can obtain the residual stressdirectly through measuring the propagation time of ultrasonic wave in the material, is presented. At first, theprinciple of the measuring method of residual stress based on UTM is analyzed. Then the measuring systemof the method is described, which is in virtue of ultrasonic flaw detector and high-sampling-rate digitaloscillograph. And a set of calibration system that contains a piece of standard specimen is also introduced.Experimental results prove the relation between the residual stress and the propagation time of ultrasonic inworkpieces. Finally, the measuring and calibration systems are applied in evaluating the effect of VSR. Thefinal test results show that the method is effective. | He Wen Ma Zhenyu Wang Xingli | 2005 | Chinese Journal of Mechanical Engineering2005，18，3： | 4 |
| 6 | Evaluation of coronary plaque and stent deployment by intravascular optical coherence tomography in elderly patients with unstable angina and non-ST-elevation myocardial infarction显示文摘Objective To evaluate the feasibility and efficacy of intravascular optical coherence tomography (OCT) in the assessment of plaque characteristics and drug eluting stent deployment quality in the elderly patients with unstable angina (UA) and non-ST segment elevation myocardial infarction (NSTEMI). Methods OCT was used in elderly patients undergoing percutaneous coronary interventions. Fifteen patients, 9 males and 6 females with mean age of 72.6±5.3 years (range 67-92 years) were enrolled in the study. Images were obtained before initial balloon dilatation and following stent deployment. The plaque characteristics before dilation, vessel dissection, tissue prolapse, stent apposition and strut distribution after stent implantation were evaluated. Results Fifteen lesions were selected from 32 angiographic lesions as study lesions for OCT imaging after diagnostic coronary angiography. There were 7 lesions in the left anterior descending artery, 5 lesions in the right coronary artery and 3 lesions in the left circumflex coronary artery. Among them, 12 (80.0%) were lipid-rich plaques, and 10 (66.7%) were vulnerable plaques with fibrous cap thickness 54.2±7.3 μm. Seven ruptured culprit plaques (46.7%) were found; 4 in UA patients and 3 in NSTEMI patients. Tissue prolapse was observed in 11 lesions (73.3%). Irregular stent strut distribution was detected in 8 lesions (53.3%). Vessel dissections were found in 5 lesions (33.3%). Incomplete stent apposition was observed in 3 stents (20%) with mean spacing between the struts and the vessel wall 172±96 mm (range 117-436 mm). Conclusions 1) It is safe and feasible to perform intravascular OCT to differentiate vulnerable coronary plaque and monitor stent deployment in elderly patients with UA and USTEMI. 2) Coronary plaques in elderly patients with UA and USTEMI could be divided into acute ruptured plaque, vulnerable plaque, lipid-rich plaque, and stable plaque. 3) Minor or critical plaque rupture is one of the mechanisms of UA in elderly patients. 4) Present drug eluting stent implantation is complicated with multiple tissue prolapses which are associated with irregular strut distributions. 5) The action and significance of tissue prolapse on acute vessel flow and in-stent thrombus and restenosis need to be further studied. | Caiyi LU Shiwen WANG Wei YAN Xingli WU Yuxiao ZHANG Qiao XUE Muyang YAN Peng LIU Rui CHEN Jinyue ZHAI | 2007 | Journal of Geriatric Cardiology2007，4，1： | 3 |
| 7 | Risk factors for the incidence of apoplexy in pituitary adenoma: a single-center study from southwestern China显示文摘Background:Although the incidence and clinical manifestations of pituitary apoplexy were reported by a few researches,the results are not consistent.This study aimed to explore the risk factors associated with an incidence of apoplexy in pituitary adenomas.Methods:The clinical information of 843 patients with pituitary adenoma from the Department of Neurological Surgery,1st Affiliated Hospital of Kunming Medical University,was reviewed.The incidence,clinical manifestation,and potential risk factors for pituitary apoplexy were analyzed by a case-control study.Results:In total,121 patients(14.4%)with macroadenoma were suffered from pituitary apoplexy.Headache,vomiting,and visual impairment are the top 3 symptoms for the pituitary apoplexy.Logistic regression results showed that the hypertension(hypertension vs non-hypertension OR=2.765,95%CI:1.41~5.416),tumor type(negative staining vs.positive staining,OR=1.501,95%CI:1.248~5.235),and tumor size(diameter>2 cm vs.diameter≤2 cm,OR=3.952,95%CI:2.211~7.053)are independent factors associated with pituitary apoplexy.Conclusion:Our results indicate that the risk factors for the incidence of pituitary apoplexy depend mainly on properties of the tumor itself(tumor size and pathologic type)and the blood pressure of patients. | Yao Li Yuan Qian Yisheng Qiao Xiaoxiang Chen Jiaotian Xu Chao Zhang Wei Wang Junjun Li Xingli Deng | 2020 | Chinese Neurosurgical Journal2020，6，4： | 3 |
| 8 | Elasto-plastic analysis of the surrounding rock mass in circular tunnel based on the generalized nonlinear unified strength theory显示文摘The present paper aims to establish a versatile strength theory suitable for elasto-plastic analysis of underground tunnel surrounding rock. In order to analyze the effects of intermediate principal stress and the rock properties on its deformation and failure of rock mass, the generalized nonlinear unified strength theory and elasto-plastic mechanics are used to deduce analytic solution of the radius and stress of tunnel plastic zone and the periphery displacement of tunnel under uniform ground stress field. The results show that: intermediate principal stress coefficient b has significant effect on the plastic range,the magnitude of stress and surrounding rock pressure. Then, the results are compared with the unified strength criterion solution and Mohr–Coulomb criterion solution, and concluded that the generalized nonlinear unified strength criterion is more applicable to elasto-plastic analysis of underground tunnel surrounding rock. | Huang Xiujie Zhang Jixun Yang Ling Yang Shikou Wang Xingli | 2016 | International Journal of Mining Science and Technology2016，26，5： | 2 |
| 9 | Valley polarization in stacked MoS2 induced by circularly polarized light显示文摘山谷 pseudospins 的操作为未来 valleytronics 是关键的。新兴的转变金属 dichalcogenides (TMD ) 为在自由,包括的真实旋转,山谷 pseudospin,和层 pseudospin 的量度之中探索相互影响提供新可能性。例如,联合的纺纱山谷导致有详细规格的电子在旋转的山谷依赖者通报二色性(起来或下面) 能被 chiral 有选择地激动光,在很少层 TMD 在单层 TMD 抽,进一步跳跃的夹层影响联合的纺纱山谷。除了山谷和层 pseudospins,这里,我们建议 freedomstacking pseudospinand 的新度证明新现象相关到这新叠自由不那样要求申请外部电或磁场。我们调查了 chemical-vapor-deposition-grown trilayer 瞬间 2(AAA,纬纱, AAB,骆驼毛的织物,和 3R ) 的所有可能的叠配置。尽管 AAA,骆驼毛的织物, 3R 叠比在单层样品, AAB (纬纱) 叠展出二座不同山峰的与山谷极化的更低的度拥有一座唯一的山峰,类似于在单层瞬间 2 观察了那的和另外的 unpolarized 在更低的精力达到顶点。我们的调查结果为未来 valleytronics 提供山谷量控制的更完全的理解。 | Juan Xia Xingli Wang Beng Kang Tay Shoushun Chen Zheng Liu Jiaxu Yan Zexiang Shen | 2017 | Nano Research2017，10，5： | 2 |
| 10 | miR-145 Inhibits Migration and Invasion of Glioma Stem Cells by Targeting ABCG2显示文摘 | Lei Shi Zhimin Wang Guan Sun Yi Wan Jun Guo Xingli Fu | 2014 | NeuroMolecular Medicine2014，，2： | 1 |
| 11 | A meta-analysis of the effects of statins on serum C-reactive protein in Chinese population with coronary heart disease or hyperlipidemia显示文摘There is little doubt that statins reduce cardiovascular events more than what the lipid lowering effect can account for. Additional mechanisms have been postulated including the anti-inflammatory effects manifested by reduced C-reactive protein (CRP). It is not known, however, whether statins can decrease CRP in Chinese population. The aim of this study is to investigate the effects of statins on serum CRP in Chinese patients with coronary heart disease (CHD) or hyperlipidemia. Trials were retrieved through Medline (1980 to May, 2009), bibliographies, and the author’s reference files limited to English-language articles. Data were extracted and meta-analysis was performed. Analysis showed statistically significant reduction in CRP after statin treatment (weighted mean difference [WMD] = -0.73, 95% confidence interval [CI] = [-0.80, -0.66], P < 0.00001) and lower CRP after statin treatment than non-statin routine treatment ([WMD] = -0.52, 95% CI [-0.86, -0.18], P = 0.002). In conclusion, statins significantly reduce serum CRP in Chinese population, which may contribute to statin-induced reduction in the cardiovascular risk in addition to the lipid lowering effect. | HAO PanPan CHEN YuGuo WANG XingLi XU Feng WANG JiaLi ZHANG Yun | 2009 | Chinese Science Bulletin2009，54，23： | 1 |
| 12 | Integrating phenotypic features and tissue-specific information to prioritize disease genes显示文摘Prioritization of candidate disease genes is crucial for improving medical care, and is one of the fundamental challenges in the post-genomic era. In recent years, different network-based methods for gene prioritization are proposed. Previous studies on gene prioritization show that tissue-specific protein-protein interaction(PPI) networks built by integrating PPIs with tissue-specific gene expression profiles can perform better than tissue-na¨?ve global PPI network. Based on the observations that diseases with similar phenotypes are likely to have common related genes, and genes associated with the same phenotype tend to interact with each other, we propose a method to prioritize disease genes based on a heterogeneous network built by integrating phenotypic features and tissue-specific information. In this heterogeneous network, the PPI network is built by integrating phenotypic features with a tissue-specific PPI network, and the disease network consists of the diseases that are associated with the same phenotype and tissue as the query disease. To determine the impacts of these two factors on gene prioritization, we test three typical network-based prioritization methods on heterogeneous networks consisting of combinations of different PPIs and disease networks built with or without phenotypic features and tissue-specific information. We also compare the proposed method with other tissuespecific networks. The results of case studies reveals that integrating phenotypic features with a tissue-specific PPI network improves the prioritization results. Moreover, the disease networks generated using our method not only show comparable performance with the widely used disease similarity dataset of 5080 human diseases,but are also effective for diseases that are not in the dataset. | Yue DENG Lin GAO Xingli GUO Bingbo WANG | 2016 | Science China(Information Sciences)2016，59，7： | 1 |
| 13 | Morphology, Thermal Behavior, and Mechanical Properties of PAl010/ PP/PP-g-GMA Blends显示文摘 | Zhang Xiaomin Li Xingli Wang Dongmei | 1997 | Journal of Applied Polymer Science1997，64，： | 1 |
| 14 | Programming of Regulatory T Cells In Situ for Nerve Regeneration and Long-Term Patency of Vascular Grafts显示文摘Rapid integration into the host tissue is critical for long-term patency after small diameter tissue engineering vascular grafts(sdTEVGs)transplantation.Neural recognition may be required for host integration and functionalization of the graft.However,immune rejection and inflammation hinder nerve regeneration of sdTEVGs.Here,a CRISPR/dCas9-nanocarrier was used for targeted programming of regulatory T cells(Treg cells)in situ to promote nerve regeneration of sdTEVGs by preventing excessive inflammation.Treg cells and(C-C chemokine receptor)CCR2+macrophage recruitment occurred after transplantation.The nanodelivery system upregulated ten eleven translocation(TET2)in Treg cells in vitro.Reprogrammed Treg cells upregulated anti-inflammatory cytokines and decreased the proportion of CCR2+macrophages.IL-6 concentrations decreased to the levels required for nerve regeneration.Implantation of CRISPR/dCas9 nanodelivery system-modified sdTEVGs in rats resulted in Treg cell editing,control of excessive inflammation,and promoted nerve regeneration.After 3 months,nerve regeneration was similar to that observed in normal blood vessels;good immune homeostasis,consistency of hemodynamics,and matrix regeneration were observed.Neural recognition promotes further integration of the graft into the host,with unobstructed blood vessels without intimal hyperplasia.Our findings provide new insights into vascular implant functionalization by the host. | Yanhong Wang Fangchao Xue Yanzhao Li Lin Lin Yeqin Wang Shanlan Zhao Xingli Zhao Yong Liu Ju Tan Gang Li Haoran Xiao Juan Yan Hao Tian Min Liu Qiao Zhang Zhaojing Ba Lang He Wenyan Zhao Chuhong Zhu Wen Zeng | 2022 | Research2022，，4： | 1 |
| 15 | Metformin reduces intracellular reactive oxygen species levels by upregulating expression of the antioxidant thioredoxin via the AMPK-FOXO3 pathway显示文摘 | Xinguo Hou Jun Song Xiao-Nan Li Lin Zhang XingLi Wang Li Chen Ying H. Shen | 2010 | Biochemical and Biophysical Research Communications2010，，2： | 1 |
| 16 | 查看详情显示文摘 | Wang Xingli Li Zongwei Chen Linha | | 0，，： | 1 |
| 17 | Reducing Viral Inhibition of Host Cellular Apoptosis Strengthens the Immunogenicity and Protective Efficacy of an Attenuated HSV-1 Strain显示文摘Herpes simplex virus 1(HSV-1),a member of a herpesviruses,shows a high infectivity rate of 30%-60%in populations of various ages.Some herpes simplex(HSV)vaccine candidates evaluated during the past 20 years have not shown protective efficacy against viral infection.An improved understanding of the immune profile of infected individuals and the associated mechanism is needed.HSV uses an immune evasion strategy during viral replication,and various virus-encoded proteins,such as ICP47 and Vhs,participate in this process through limiting the ability of CD8+cytotoxic T lymphocytes to recognize target cells.Other proteins,e.g.,Us3 and Us5,also play a role in viral immune evasion via interfering with cellular apoptosis.In this work,to study the mechanism by which HSV-1 strain attenuation interferes with the viral immune evasion strategy,we constructed a mutant strain,M5,with deletions in the Us3 and Us5 genes.M5 was shown to induce higher neutralizing antibody titers and a stronger cellular immune response than our previously reported M3 strain,and to prevent virus infection more effectively than the M3 strain in an in vivo mouse challenge test. | Xingli Xu Yufeng He Shengtao Fan Min Feng Guorun Jiang Lichun Wang Ying Zhang Yun Liao Qihan Li | 2019 | Virologica Sinica2019，34，6： | 1 |
| 18 | Effects of Food Additives on Rehydration Properties of Frozen Dumpling Wrappers显示文摘With the vacuum freeze-drying technology,frozen dumpling wrappers were prepared,to investigate the effects of six kinds of food additives,including modified starch,compound phosphate,maltodextrin,guar gum,distilled monoglycerides and transglutaminase( TG enzyme),on the drying rate,rehydration ratio and sense value of the frozen dumpling wrappers. The results showed that,with respective addition of 6% modified starch,0. 1% compound phosphate,10% maltodextrin,0. 4% guar gum,0. 4% distilled monoglyceride and 0. 3% transglutaminase,the drying rate,rehydration ratio and sense value of the frozen dumpling wrappers were the highest. | Hua LI Xingli JIAO Xiang GUI Ying XIONG Junling LI Weihua WANG Feng CAO | 2014 | Agricultural Biotechnology2014，3，6： | 1 |
| 19 | miR-125b inhibitor enhance the chemosensitivity of glioblastoma stem cells to temozolomide by targeting Bak1显示文摘 | Jian Chen Xingli Fu Yi Wan Zhimin Wang Dongyi Jiang Lei Shi | 2014 | Tumor Biology2014，，7： | 1 |
| 20 | A study on powder X-ray diffraction of Ti3SiC2 显示文摘 | Tang K Wang Changan Xu Xingli | 2002 | Mater Lett2002，55，： | 1 |
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