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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Whole brain radiotherapy for the treatment of multiple brain metastases显示文摘 | Tsao M Lloyd N Wong R Chow E Rakovitch E Laperriere N | 2006 | 中国神经肿瘤杂志2006，4，2： | 17 |
| 3 | 单髁与全膝关节置换治疗内侧间室膝骨关节炎:5年假体在位率比较显示文摘背景:单髁置换越来越多的被应用于治疗单纯内侧胫股间室型膝关节骨关节炎。目的:比较膝关节单髁置换与全膝关节置换对膝关节单纯内侧间室骨关节炎的修复效果。方法:纳入单纯内侧间室型膝关节骨关节炎患者109例,根据修复方式分为两组,单髁置换组41例,全膝关节置换组68例。比较两组患者的平均住院时间、置换后并发症发生率、身体健康总评分、精神健康总评分、牛津膝关节评分以及置换后5年假体在位率。结果与结论:单髁置换组患者平均住院时间(4.21±2.44)d,较全膝关节置换组(6.11±2.23)d短(P=0.001)。两组患者置换后并发症发生率、身体健康总评分、精神健康总评分、牛津膝关节评分等方面差异均无显著性意义(P>0.05)。置换后5年,单髁置换组假体在位率(90%)较全膝关节置换组(100%)低(P<0.05)。提示对于单纯内侧胫股间室型膝关节骨关节炎患者,单髁置换与全膝关节置换具有相似的修复效果,但单髁置换后5年假体在位率稍低。 | 梁宁 Samuel Yih Shyan Wong Nick Clement Deborah MacDonald Daniel E Porter | 2015 | 中国组织工程研究2015，19，53： | 12 |
| 4 | Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物显示文摘为了抵抗疾病,很多医药库中的武器是从细菌当中获得的。如今,利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。 | Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao Fong Tian Wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim | 2017 | 现代生物医学进展2017，17，17： | 12 |
| 5 | Some statistical properties of median filters显示文摘 | Ataman E Aatre V K Wong K M | 1980 | IEEE Trans on Acoustics Speech Signal Processing1980，28，4： | 2 |
| 6 | Acute sensorineural hearing loss associated with peginterferon and ribavirin combination therapy during hepatitis C treatment: Outcome after resumption of therapy显示文摘Peginterferon and ribavirin combination therapy for the treatment of hepatitis C virus (HCV) is well known to be associated with significant adverse effects. Sensorineural hearing loss, that in most cases is unilateral, has been reported as a consequence of therapy with both non-pegylated and pegylated interferon (pegIFN) but is not a well-known adverse effect. We report a 45-year-old Caucasian woman who developed acute sensorineural hearing loss 2 mo after starting therapy with pegIFN-α 2b and ribavirin for the treatment of chronic HCV, genotype 1a. She did not report the hearing loss to the hepatitis clinic until L mo,later whereupon therapy was promptly discontinued.Although her serum alanine aminotransferase (ALT)normalized and her HCV-RNA became undetectable after 12 wk of pegIFN and ribavirin therapy, after discontinuation,her HCV-RNA became detectable with significant elevations of serum ALT. Four months after initial discontinuation,the patient re-commenced pegIFN and ribavirin combination therapy. After 44 of 48 wk of therapy, the patient's liver biochemistry has normalized and the HCV-RNA is undetectable. She has not developed worsening of her hearing loss and hearing on the left-side is unaffected.Both patients and physicians should be aware that sensorineural hearing loss may occur with pegIFN therapy.Our experience suggests that re-institution of therapy is not always associated with further hearing impairment. | Victor K Wong Cindy Cheong-Lee Jo-Ann E Ford Eric M Yoshida | 2005 | World Journal of Gastroenterology2005，11，34： | 2 |
| 7 | Noise-induced hearing loss in the 21^(st) century: A research and translational update显示文摘Millions of people worldwide are exposed to harmful levels of noise daily in their work and leisure environment. This makes noise-induced hearing loss(NIHL) a major occupational health risk globally. NIHL is the second most common form of acquired hearing loss after agerelated hearing loss and is itself a major contributing factor to presbycusis. Temporary threshold shifts, once thought to be relatively harmless and recoverable, are now known to cause permanent cochlear injury leading to permanent loss of hearing sensitivity. This article reviews the current understanding of the cellular and molecular pathophysiology of NIHL with latest findings from animal models. Therapeutic approaches to protect against or to mitigate NIHL are discussed based on their proposed action against these known mechanisms of cochlear injury. Successes in identifying genes that predispose individuals to NIHL by candidate gene association studies are discussed with matched gene knockout animal models. This links to exciting developments in experimental gene therapy to replace and regenerate lost hair cells and post-noise otoprotective therapies currently being investigated in clinical trials. The aim is to provide new insights into current and projected future strategies to manage NIHL; bench to bedside treatment is foreseeable in the next 5 to 10 years. | Ann Chi Yan Wong Kristina E Froud Yves Shang-Yi Hsieh | 2013 | World Journal of Otorhinolaryngology2013，3，3： | 2 |
| 8 | A new approach to using anisotropically conductive adhesives for flip-chip assembly显示文摘 | Lyons A M Hall E Wong Y H | 1996 | IEEE Trans Compon Packg Manuf Techn Part A1996，19，1： | 2 |
| 9 | A comparison of soil-Structure interaction calculations with results of full-scale forced vibration test显示文摘 | WONG H L LUCO J E | 1988 | J Soil Dyn & Earth Engng1988，7，1： | 1 |
| 10 | GATA-1 and Oct-1 are required for expression of the human alpha-hemoglobin-stabilizing protein gene 显示文摘 | Gallagher P G Liem R I Wong E | 2005 | J Biol Chem2005，280，39： | 1 |
| 11 | Dynamic response of a coupled spinning Timoshenko shaft system显示文摘 | Zu J W | 1999 | Journal of Vibration and Acoustics1999，121，1： | 1 |
| 12 | Nanobeam mechanics: Elasticity, strength, and toughness of nanorods and nanotubes显示文摘 | Sheehan P E Lieber C M | 1997 | Science1997，，277： | 1 |
| 13 | Effective program debugging based on execution slices and inter-block data dependency显示文摘 | Wong W E Qi Y | 2006 | Journal of Systems and Software2006，79，7： | 1 |
| 14 | 显示文摘 | Kai-hoi SF Wong E Lo R | 2000 | Palliat Med2000，14，： | 1 |
| 15 | Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages:role of inhibition of NF- κB activation 显示文摘 | E Gilad HR Wong B Zingarelli | 1998 | FASEB J1998，12，： | 1 |
| 16 | Formation processes of silica nanotubes through a surfactant-assisted templating mechanism in laurylamine hydrochloride/tetraethoxysilane system 显示文摘 | Dai H Wong E W Lieber C M | 1995 | Nature1995，375，6534： | 1 |
| 17 | Peptide absorption anti utiliza- tion: Implications for animal nutrition and health显示文摘 | Gilbert E R Wong E Webb K E | 2008 | Jourual of Animal Science2008，86，： | 1 |
| 18 | Ebola virus entry requires the cholesterol transporter Niemann-Pick Cl显示文摘 | Carette J E Raaben M Wong A C | | 0，，7364： | 1 |
| 19 | Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2012-2013显示文摘 | Meijer A Rebelo-de-Andrade H Correia V Besselaar T Drager-Dayal R Fry A Gregory V Gubareva L Kageyama T Laekenby A Lo J Odagiri T Pere- yaslov D Siqueira M M Takashita E Tashiro M Wang D Wong S Zhang W Daniels R S Hurt A C | 2014 | An- tiviral Research2014，110，： | 1 |
| 20 | Automated/MRT planning with regional optimization using planning scripts 显示文摘 | Xhaferllari I Wong E Bzdusek K | 2013 | Med Phys2013，14，1： | 1 |
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