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| 1 | Intrahepatic transplantation of hepatic oval cells for fulminant hepatic failure in rats显示文摘AIM:To evaluate the effect of intrahepatic transplantation of hepatic oval cells(HOC)on fulminant hepatic failure(FHF)in rats.METHODS:HOC obtained from rats were labeled with green fluocescent protein(GFP)or 5,6carboxyfluorescein diacetate succinmidyl ester(CFDASE).Cell fluorescence was observed under fluorescent microscope at 6,24,48 and 72 h after labeling.CFDASE labeled HOC(5×10 6 cells each rat)were injected into livers of rats with FHF induced by D-galactosamine.Serum albumin(ALB),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBil)levels were measured at different time points.Liver function of rats was examined on days 3,7,14 and 21 after HOC transplantation.RESULTS:The positive rate of GFP and CFDA-SE labeled HOC was 10%and 90%,respectively,with no significant change in cell viabilities.The survival rate was higher in HOC transplantation group than in control group,especially 48(9/15 vs 6/15)and 72 h(9/15 vs 4/15)after HOC transplantation.The serum ALT,AST and TBil levels were decreased while the serum Alb level was increased after HOC transplantation.Fluorescence became faded and diffused in liver tissues,suggesting that proliferation and differentiation occur in transplanted HOC.CONCLUSION:CFDA-SE is superior to GFP in labeling HOC,although fluorescence intensity is decreased progressively with cell division.HOC transplantation can improve the liver function and increase the survival rate of recipients. | Chen-Xuan Wu,Qi Zou,Zheng-Yan Zhu,Ying-Tang Gao,Yi-Jun Wang,Department of Hepatobiliary,The Third Center of Affiliated Hospital of Tianjin Medical University Key Laboratory of Artificial Cells of Tianjin,Tianjin 300170,China Author contributions:Wang YJ,Wu CH and Zou Q designed the research Zou Q,Zhu ZY,Gao YT performed the research Zou Q analyzed the data Wang YJ and Wu CX wrote the paper.Supported by Tianjin Science Committee,Grant No.05SYSYJC02600 and Tianjin Health Bureau,Grant No.05KYR01 | 2009 | World Journal of Gastroenterology2009，15，12： | 7 |
| 2 | Stellarins F and G, two new cyclopeptides from Stellaria yunnanensis显示文摘In the previous papers,we reported the structural elucidation of stellarins A-E,fivenew cyclopeptides from the fresh roots of Stellaria yunnanensis Franch.Further chemical study on this plant led to the isolation of two other new cyclopeptides named stellarins F(l) and G(2).By a combination of 2D-NMR,enzymatic degradation and FABMS,their structures were established to be cyclo(Gly-Ala-Gly-Ser-Pro-Trp-Phe-Pro) and cyclo(Gly-Ala-Tyr-Leu-Ala),respectively. | Zhao, YR Wang, XK Zhou, J Cheng, CX Huang, XL Wu, HM | 1995 | Chinese Journal of Chemistry1995，13，6： | 3 |
| 3 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 4 | Enhanced pulmonary inflammation following experimental intracerebral hemorrhage显示文摘 | Wu S Fang CX Kim J | 2006 | Exp Neurol2006，200，1： | 1 |
| 5 | Enhanced pulmonary inflam- mation following experimental intracerebral hemorrhage 显示文摘 | Wu S Fang CX Kim J | 2006 | Exp Neurol2006，200，1： | 1 |
| 6 | Enhanced pulmonary inflammation following experimental intracerebral hemorrhage显示文摘 | Wu S Fang CX Kim J | 2006 | Exp Neurol2006，200，1： | 1 |
| 7 | Frequencies of GSTM1,GSTT1,and GSTP1 polymorphisms in ethnic minority from Guizhou显示文摘 | Zhang W Shan KR Wu CX | 2007 | Zhonghua Yi Xue Yi Chuan Xue Za Zhi2007，24，5： | 1 |
| 8 | Application of dispersion 'solid- ification liquid - liquid microextraction for the determination of tria- zole fungicides in environmental water samples by high - perform- ance liquid chromatography显示文摘 | Wang C Wu QH Wu CX | 2011 | J Hazard Mater2011，185，1： | 1 |
| 9 | Alterations of nAChRs and ERK1/2 in the brains of rats with chronic fluorosis and their connections with the decreased capacity of learning and memory显示文摘 | Liu YJ Gao Q Wu CX | | 0，，03： | 1 |
| 10 | Alternations of nAChRs and ERK1/2 in the brains of rats with chronic fluorosis and their connections with the decreased capacity of learning and memory显示文摘 | Liu YJ Gao Q Wu CX | 2010 | Toxicol Lett2010，192，3： | 1 |
| 11 | Effectiveness of multiple daily injections or continuous subcutaneous insulin infusion for children with type 1 diabetes mellitus in clinical practice显示文摘 | Gong CX Wei LY Wu D | 2014 | Int J Endocrinol2014，2014，52： | 1 |
| 12 | Peritumoral edema on magnetic resonance imaging predicts a poor clinical outcome in malignant glioma显示文摘 | Wu CX Lin GS Lin ZX | 2015 | Oncol Lett2015，10，5： | 1 |
| 13 | Alternations of nAChRs and ERK1/2 in the brains of rats with chronic fluorosis and their connections with the decreased capacity of learning and memory显示文摘 | Liu YJ Gao Q Wu CX | | 0，，03： | 1 |
| 14 | Evaluation of antiinflammatory activity of the total flavonoids of Laggera pterodonta on acute and chronic inflammation models显示文摘 | Wu YH Zhou CX Li XP | | 0，，7： | 1 |
| 15 | Intestinal damage mediated by Kupffer cells in rats with endotoxemia 显示文摘 | Wu CX Liu CA | 2002 | World J Gastroenterol2002，8，5： | 1 |
| 16 | Clinical singnificance of ST segment elevalation in leadIII greater than lead II in patients with acute inferior myocardial infarction显示文摘 | Wu SL Huang CX Wang JM | | 0，，： | 1 |
| 17 | Alterations of nACHRs and ERK1/2 in the brains of rats with chronic fluorosis and their connections with the decreased capacity of learning and memory 显示文摘 | Liu YJ Gao Q Wu CX | 2010 | Toxicol Lett2010，192，3： | 1 |
| 18 | Soil persistenceof Bacillus thuringiensis (Bt) toxin from transgenicBt cotton tissues and its effect on soil enzymeactivities 显示文摘 | Sun CX Chen LJ Wu ZJ | 2007 | Biology and Fertility of Soils2007，43，： | 1 |
| 19 | Effect of electroacupuncture on cell apoptosis and ERK signal pathway in the hippocampus of a- dult rats with cerebral ischemia-reperfusion 显示文摘 | Wu CX Wang J Li C | 2015 | Evid Based Complement Alternat Med2015，，41： | 1 |
| 20 | Hippocampal nitric oxide contributes to sex difference in affective behaviors 显示文摘 | Hu Y Wu DL Luo CX | 2012 | Proc Natl Acad Sci USA2012，109，14： | 1 |
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