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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | Physical and chemical stability of drug nanoparticles 显示文摘 | Wu LB Zhang J Watanabe W | 2011 | Adv Drug Deliv Rev2011，63，6： | 1 |
| 3 | In vitro degradation of three-dimensional porous poly (D,L-lactide-co-glycolide) scaffolds for tissue engineering 显示文摘 | Wu LB Ding JD | 2004 | Biomaterials2004，25，27： | 1 |
| 4 | Upregulator of cell prolifer-ation predicts poor prognosis in hepatocellular carcinoma and contributes to hepatocarcinogenesis by downregulating FOXO3a显示文摘 | Xie C Song LB Wu JH | 2012 | PLoS One2012，7，40： | 1 |
| 5 | Structure determination ofanovel natural product isolated from the leaves of Panaxginseng CAMeyer显示文摘 | Wang LB Gao HY Wu B | 2006 | Chin J Magn Reson2006，23，3： | 1 |
| 6 | Building an innovation electronic nursing record pilot structure with nursing clinical pathway显示文摘 | Hao AT Huang LF Wu LB | 2006 | Stud Health Technol Inform2006，122，： | 1 |
| 7 | Driving Forces behind the Stagnancy of China' s Energy--related COz Emissions from 1996 to 1999: the Relative Importance of Structural Change, Intensity Change and Scale Change 显示文摘 | WU LB KANEKO S MATSUOKA S | 2005 | Energy Policy2005，，33： | 1 |
| 8 | CaM kinase augments cardiac L-type Ca2+ current: A cellular mechanism for long Q-T arrhythmias显示文摘 | Wu Y MacMillan LB McNeill RB | 1999 | Am J Physiol1999，276，： | 1 |
| 9 | Computerized tomography pleurography: a new method for detecting minor pleural lesion显示文摘 | Gong RZ Wu LB | 2001 | Chin Med J2001，114，9： | 1 |
| 10 | Building an innovation electronic nursing record pilot structure with nursing clinical pathway显示文摘 | Hao AT Huang LF Wu LB | | 0，，： | 1 |
| 11 | Single-incision laparoscopic cholecystectomy versus multi-incision laparoscopic cholecystectomy:a meta-analysis of randomized clinical trials显示文摘 | Wu XS Shi LB Gu J | 2013 | J Laparoendosc Adv Surg Tech A2013，23，3： | 1 |
| 12 | Single-incision laparoscopic cholecystectomyversus multi- incision laparoscopic cholecystectomy:a metaanalysis of randomized clinical triMs显示文摘 | Wu XS Shi LB Gu J | 2013 | J Laparoendoscopic Advanced Surgical Techniques2013，23，3： | 1 |
| 13 | A survey of blindness and cataract surgery in Doumen County, China 显示文摘 | Li S Xu J He M Wu K Munoz SR Ellwein LB | 1999 | Ophthalmology1999，106，8： | 1 |
| 14 | Effects of estrogen replacement on the relative levels of choline acetyltransferase,trkA, and nerve growth factor messenger RNAs in the basal forebrain and hippocampal formation of adult rats显示文摘 | Wu D Hersh LB | 1994 | Exp Neurol1994，129，1： | 1 |
| 15 | Single - incision laparoscopic cholecys - tectomy versus multi - incision laparoscopic cholecystectomy: ameta - analysis of randomized clinical trims 显示文摘 | Wu XS Shi LB Gu J | 2013 | J Laparoendoscopic Advanced Surgical Techniques2013，23，3： | 1 |
| 16 | Clinical application of 64-slice spiral CT in the diagnosis of the tetralogy of fallot显示文摘 | Wang XM Wu LB Sun C | 2007 | Eur J Radiol2007，64，： | 1 |
| 17 | Upregulator of cell proliferation predicts poor prognosis in hepatocellular carcinoma and contributes to hepatocarcinogenesis by downregulating FOXO3a显示文摘 | Xie C Song LB Wu JH | 2012 | PLoS One2012，7，40： | 1 |
| 18 | SYNTHESIS OF FULLERENE PYRROLIDINES CONTAINING SUBSTITUTED PHENYL GROUPS THROUGH AZOMETHINE YLIDE ADDITION显示文摘SYNTHESIS OF FULLERENE PYRROLIDINES CONTAINING SUBSTITUTED PHENYL GROUPS THROUGH AZOMETHINE YLIDE ADDITIONDeJianZHOU;LiangBin... | Zhou, DJ Gan, LB Tan, HS Luo, CP Huang, CH Pan, JQ Lu, MJ Wu, Y | 1995 | Chinese Chemical Letters1995，6，12： | 1 |
| 19 | Hydrogen sulfide as a potent cardiovascular protective agent显示文摘 | Yu XH Cui LB Wu K | 2014 | Clin Chim Acta2014，437，： | 1 |
| 20 | Factors contributing to the high prevalence of multidrug-resistant tuberculosis:a study from China显示文摘 | Liang LB Wu QH Gao LJ | 2012 | Thorax2012，67，7： | 1 |
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