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| 1 | Immunization with chlamydial plasmid protein pORF5 DNA vaccine induces protective immunity against genital chlamydial infection in mice显示文摘To validate the immune protective efficacy of pORF5 DNA vaccine and to analyze potential mechanisms related to this protection. In this study, pORF5 DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial infection. Groups of BALB/c mice were immunized intranasally with pORF5 DNA vaccine. Humoral and cell mediated immune responses were evaluated. The clearance ability of chlamydial challenge from the genital tract and the chlamy- dia-induced upper genital tract gross pathology and histopathological characterization were also de- tected. The results showed that the total and the IgG2a anti-pORF5 antibody levels in serum were sig- nificantly elevated after pcDNA3.1-pORF5 vaccination, as were the total antibody and IgA levels in vaginal fluids. pcDNA3.1-pORF5 induced a significantly high level of Th1 response as measured by robust gamma interferon (IFN-γ). Minimal IL-4 was produced by immune T cells in response to the re-stimulation with pORF5 protein or the inactive elementary body in vitro. pcDNA3.1-pORF5-vacci- nated mice displayed significantly reduced bacterial shedding upon a chlamydial challenge and an accelerated resolution of infection. 100% of pcDNA3.1-pORF5 vaccinated mice successfully resolved the infection by day 24. pcDNA3.1-pORF5-immunized mice also exhibited protection against patho- logical consequences of chlamydial infection. The stimulated index was significantly higher than that of mice immunized with pcDNA3.1 and PBS (P<0.05). Together, these results demonstrated that immu- nization with pORF5 DNA vaccine is a promising approach for eliciting a protective immunity against a genital chlamydial challenge. | LI ZhongYu1,2, WANG ShiPing1, Wu YiMou2, ZHONG GuangMing3 & CHEN Ding3 1 Xiangya School of Medicine, Central South University, Changsha 410078, China 2 Department of Microbiology and Immunology, University of South China, Hengyang 421001, China 3 University of Texas Health Science Center at San Antonio, TX 78229, USA | 2008 | Science China(Life Sciences)2008，51，11： | 16 |
| 2 | Automated detection of lunar craters based on object-oriented approach显示文摘The object-oriented approach is a powerful method in making classification. With the segmentation of images to objects, many features can be calculated based on the objects so that the targets can be distinguished. However, this method has not been applied to lunar study. In this paper we attempt to apply this method to detecting lunar craters with promising results. Craters are the most obvious features on the moon and they are important for lunar geologic study. One of the important questions in lunar research is to estimate lunar surface ages by examination of crater density per unit area. Hence, proper detection of lunar craters is necessary. Manual crater identification is inefficient, and a more efficient and effective method is needed. This paper describes an object-oriented method to detect lunar craters using lunar reflectance images. In the method, many objects were first segmented from the image based on size, shape, color, and the weights to every layer. Then the feature of 'contrast to neighbor objects' was selected to identify craters from the lunar image. In the next step, by merging the adjacent objects belonging to the same class, almost every crater can be taken as an independent object except several very big craters in the study area. To remove the crater rays diagnosed as craters, the feature of 'length/width' was further used with suitable parameters to finish recognizing craters. Finally, the result was exported to ArcGIS for manual modification to those big craters and the number of craters was acquired. | YUE ZongYu1,2,3,4, LIU JianZhong3 & WU GanGuo2 1 Institute of Remote Sensing Application, Chinese Academy of Sciences, Beijing 100101, China 2 China University of Geosciences (Beijing), Beijing 100083, China 3 National Astronomical Observatories of Chinese Academy of Sciences, Beijing 100012, China 4 Department of Geological Sciences, The University of Texas at San Antonio, San Antonio, TX 78249, U.S.A | 2008 | Chinese Science Bulletin2008，53，23： | 12 |
| 3 | 12-Deoxyphorbol Esters from Euphorbia Fischeriana显示文摘12-DeoxyphorbolEstersfromEuphorbiaFischerianaWenZiLIU;XiaoYunWU;GenJinYANG;QingGaoMA;TianXiZHOU;XiCanTANGandGuoWeiQIN(Departm... | Liu, WZ Wu, XY Yang, GJ Ma, QG Zhou, TX Tang, XC Qin, GW | 1996 | Chinese Chemical Letters1996，7，10： | 7 |
| 4 | Recent Advances in Swine Amino Acid Nutrition显示文摘Recent advances in swine protein nutrition are characterized by the development of functional amino acids(AA)in regulating fetal and postnatal survival,growth and development.These AA include arginine,glutamine,glutamate,proline,leucine,cysteine and tryptophan.Due to limited knowledge on AA nutrition,pork producers have traditionally paid little attention to supplementing the arginine family of AA to swine diets.Results of recent studies indicate that functional AA serve important regulatory functions in nutrient metabolism,protein turnover,and immune function,therefore enhancing efficiency of feed utilization by pigs.The underlying mechanisms include activation of nitric oxide,mammalian target of rapamycin,gaseous signaling,and AMP-activated protein kinase pathways,as well as anti-oxidative function.Dietary supplementation with arginine,glutamine,proline or leucine to weanling piglets enhances their growth performance.Arginine or glutamine is also effective in increasing milk production by lactating sows.Furthermore,supplementing arginine to the diet of pregnant gilts between days 30 and 114 of gestation increases the number of live-born piglets and litter birth-weight.Availability of feed-grade functional AA holds great promise for improving animal health and nutrient utilization in pig production worldwide.Additionally,feedstuffs of animal origin [e.g.,blood meal(ring dried),feather meal(hydrolyzed),meat and bone meal,porcine protein meal,and poultry by-product meal(both feed-and petfood-grades)] are excellent and cost-effective sources of both essential and functional AA for formulating balanced swine diets.New knowledge on AA nutrition provides a much needed scientific basis for revising the next edition of swine nutrient requirements. | Guoyao Wu Department of Animal Science and Faculty of Nutrition,Texas A&M University,College Station,TX,77843,U.S.A and State Key Laboratory of Animal Nutrition,China Agricultural University,Beijing,100193,China | 2010 | Journal of Animal Science and Biotechnology2010，1，2： | 7 |
| 5 | Review of adverse reactions to chi- nese medicine injections显示文摘 | Bian Z Shang HC Wu TX | 2010 | Chin J Evid-based Med2010，10，2： | 1 |
| 6 | Traditional chinese medicine syndrome distribution in chronic hepatitis B populations:a systematic review显示文摘 | Zeng XX Bian ZX Wu TX | 2011 | Amer J Chin Med2011，39，6： | 1 |
| 7 | On torsional stiff-ness and natural frequency of bellows 显示文摘 | LuCL Wu TX YuJ G | 2004 | Journal of Mechanical Engineering Science2004，218，3： | 1 |
| 8 | Steroid withdrawal increases risk of acute rejection but reduces infection: a meta-analysis of 1681 cases in renal transplantation 显示文摘 | Tan JY Zhao N Wu TX at al | 2006 | Transplant Proc2006，38，7： | 1 |
| 9 | Firmness and force relaxation characteristics of tomatoes stored intact or asslices显示文摘 | Wu TX Abbott JA | | 0，，： | 1 |
| 10 | Effects of ginkgonealie acid on the growth, acidogenicity, adherence, and biofilm of Streptococcus mu- tans in vitro显示文摘 | He JZ Wang SD Wu TX | 2013 | Folia Microbiol2013，58，2： | 1 |
| 11 | Sprouty/FGF signaling regulates the proximal-distal feather morphology and the size of dermal papillae显示文摘 | Yue Z Jiang TX Wu P | 2012 | Dev Biol2012，372，1： | 1 |
| 12 | Efficacy of consensus in- terferon in treatment of HBeAg-positive chronic hepatitis B: amuhicentre, randomized controlled trial 显示文摘 | ZHENG YL ZHAO LS WU TX | 2009 | Virol J2009，6，14： | 1 |
| 13 | Traditional Chinese medicine syndrome distribution in chronic hepatitis B popula- tions: a systematic review显示文摘 | ZENG XX BIAN ZX WU TX | 2011 | Am J Chin Mad2011，39，6： | 1 |
| 14 | Sprouty/FGF signaling regulates the proximal-distal feather morphology and the size of dermal papillae显示文摘 | Yue Z Jiang TX Wu P | 2012 | Dev Biol2012，372，1： | 1 |
| 15 | ACE inhibitor improves insulin resistance in diabetic mouse via bradykinin and NO显示文摘 | Cui TX Wu L | 2002 | Hypertension2002，40，3： | 1 |
| 16 | The effects of Diane-35 and mefformin in treatment of polycystic ovary syndrome: an up- dated systematic review 显示文摘 | Zhang J Xu LZ Wu TX | 2008 | Gynecol Endocrinol2008，24，10： | 1 |
| 17 | ACE inhibitor improves insulin resistance in diabetic mouse via bradykin in and NO显示文摘 | Shiuchi T Cui TX Wu L | 2002 | Hypertention2002，40，3： | 1 |
| 18 | ACE inhibit or improves insulin resistance in diabetic mouse via bradykinin and NO 显示文摘 | Shiuchi T Cui TX Wu L | 2002 | Hypertension2002，40，3： | 1 |
| 19 | ACE inhibitor improves insulin resistance in diabetic mouse via bradykinin and NO 显示文摘 | Shiuchi T Cui TX Wu L | 2002 | Hypertention2002，40，3： | 1 |
| 20 | The observation of activatedplatelet in patients with coronary artery disease显示文摘 | Wu TX Zhang QL Wang W | 2003 | Practical Ger-iatrics J2003，17，： | 1 |
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