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| 1 | Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages显示文摘How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated.Here,we perform single-cell deep-immune profiling of bronchoalveolar lavage(BAL)samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung.We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them.In mild COVID-19,CD8^(+)resident-memory(TRM)and CD4^(+)T-helper-17(T_(H17))cells undergo active(presumably antigen-driven)expansion towards the end of the trajectory,and are characterized by good effector functions,while in critical COVID-19 they remain more naïve.Vice versa,CD4^(+)T-cells with T-helper-1 characteristics(TH1-like)and CD8^(+)T-cells expressing exhaustion markers(T_(EX)-like)are enriched halfway their trajectories in mild COVID-19,where they also exhibit good effector functions,while in critical COVID-19 they show evidence of inflammation-associated stress at the end of their trajectories.Monocyte-to-macrophage trajectories show that chronic hyperinflammatory monocytes are enriched in critical COVID-19,while alveolar macrophages,otherwise characterized by anti-inflammatory and antigen-presenting characteristics,are depleted.In critical COVID-19,monocytes contribute to an ATP-purinergic signaling-inflammasome footprint that could enable COVID-19 associated fibrosis and worsen disease-severity.Finally,viral RNA-tracking reveals infected lung epithelial cells,and a significant proportion of neutrophils and macrophages that are involved in viral clearance. | Els Wauters Pierre Van Mol Abhishek Dinkarnath Garg Sander Jansen Yannick Van Herck Lore Vanderbeke Ayse Bassez Bram Boeckx Bert Malengier-Devlies Anna Timmerman Thomas Van Brussel Tina Van Buyten Rogier Schepers Elisabeth Heylen Dieter Dauwe Christophe Dooms Jan Gunst Greet Hermans Philippe Meersseman Dries Testelmans Jonas Yserbyt Sabine Tejpar Walter De Wever Patrick Matthys CONTAGIOUS collaborators Johan Neyts Joost Wauters Junbin Qian Diether Lambrechts | 2021 | Cell Research2021，31，3： | 11 |
| 2 | A pan-cancer blueprint of the heterogeneous tumor microenvironment revealed by single-cell profiling显示文摘The stromal compartment of the tumor microenvironment consists of a heterogeneous set of tissue-resident and tumor-infiltrating cells,which are profoundly moulded by cancer cells.An outstanding question is to what extent this heterogeneity is similar between cancers affecting different organs.Here,we profile 233,591 single cells from patients with lung,colorectal,ovary and breast cancer(n=36)and construct a pan-cancer blueprint of stromal cell heterogeneity using different single-cell RNA and protein-based technologies.We identify 68 stromal cell populations,of which 46 are shared between cancer types and 22 are unique.We also characterise each population phenotypically by highlighting its marker genes,transcription factors,metabolic activities and tissue-specific expression differences.Resident cell types are characterised by substantial tissue specificity,while tumor-infiltrating cell types are largely shared across cancer types.Finally,by applying the blueprint to melanoma tumors treated with checkpoint immunotherapy and identifying a naive CD4+T-cell phenotype predictive of response to checkpoint immunotherapy,we illustrate how it can serve as a guide to interpret scRNA-seq data.In conclusion,by providing a comprehensive blueprint through an interactive web server,we generate the first panoramic view on the shared complexity of stromal cells in different cancers. | Junbin Qian Siel Olbrecht Bram Boeckx Hanne Vos Damya Laoui Emre Etlioglu Els Wauters Valentina Pomella Sara Verbandt Pieter Busschaert Ayse Bassez Amelie Franken Marlies Vanden Bempt Jieyi Xiong Birgit Weynand Yannick van Herck Asier Antoranz Francesca Maria Bosisio Bernard Thienpont Giuseppe Floris Ignace Vergote Ann Smeets Sabine Tejpar Diether Lambrechts | 2020 | Cell Research2020，30，9： | 11 |
| 3 | Decrease in uric acid in acute ischemic stroke correlates with stroke severity, evolution and outcome显示文摘 | Raf Brouns Annick Wauters Gerda Van De Vijver Didier De Surgeloose Rishi Sheorajpanday Peter P. De Deyn | 2010 | Clinical Chemistry and Laboratory Medicine2010，，3： | 3 |
| 4 | Induction and Maintenance Therapy With Infliximab for Children With Moderate to Severe Ulcerative Colitis显示文摘 | Jeffrey Hyams Lakshmi Damaraju Marion Blank Jewel Johanns Cynthia Guzzo Harland S. Winter Subra Kugathasan Stanley Cohen James Markowitz Johanna C. Escher Gigi Veereman–Wauters Wallace Crandall Robert Baldassano Anne Griffiths | 2012 | Clinical Gastroenterology and Hepatology2012，，4： | 3 |
| 5 | A nonmosaic 45,X karyotype in a mother with Turner's syndrome and in her daughter显示文摘 | COOLS M ROOMAN R P WAUTERS J | 2004 | Fertil Steril2004，82，4： | 1 |
| 6 | Color Doppler imaging is a valuable tool for the diagnosis and management of uterine vascular malformations显示文摘 | Timmerman D Wauters J Van Calenbergh S | 2003 | Ultrasound Obstet Gyneol2003，21，6： | 1 |
| 7 | Seasonal and site-specific variation in vapour and aerosol phase PAHs over Flanders (Belgium) and their relation with anthropogenic activities显示文摘 | Ravindra K Bencs L Wauters E | 2006 | Atmospheric Environment2006，40，： | 1 |
| 8 | An update on the referral pattern of patients with end-stage renal disease显示文摘 | Lameire N Wauters J P Teruel J L | 2002 | Kidney Int2002，50，： | 1 |
| 9 | Thermodynamic a-nalysis of reciprocating compressors 显示文摘 | Stouffs P Tazerout M Wauters P | 2001 | International Journal of Thermal Sciences2001，40，1： | 1 |
| 10 | Decrease in uric acid in acute ischemic stroke correlates with stroke severity, evolu- tion and outcome显示文摘 | Brouns R Wauters A Van De Vijver G | 2010 | Clin Chem Lab Meal2010，48，3： | 1 |
| 11 | Leptin receptor genepolymorphisms are associated with insulin in obese women with impaired glucose tolerance显示文摘 | Mertens I Rankinen T | 2001 | J Clin Endocrinol Metab2001，86，7： | 1 |
| 12 | Patient referral is influenced by dialysis center structure in the diamant alpin dialysis cohort study显示文摘 | Wauters J P Bosson J L Forneris G | 2004 | Nephrol Dial Transplant2004，19，： | 1 |
| 13 | Do female roe deer in good condition produce more sons than daughters 显示文摘 | Wauters L A Crombrugghe S A Nour N Mattgysen E | 1995 | Behav Ecol Sociobiol1995，37，： | 1 |
| 14 | Thermodynamic analysis of reciprocating compressors显示文摘 | Pascal Stouffs Mohand Tazerout Pierre Wauters | 2001 | Reciprocating compressor analysis2001，，40： | 1 |
| 15 | Preparation and characterizationof alginate microspheres containing a model antigen显示文摘 | LEMOINE D WAUTERS F BOUCHEND'HOMME S | 1998 | Int J Pharm1998，176，： | 1 |
| 16 | Soil biodegradation and leaf transfer of insecticide imidacloprid applied in seed dressing in sugar-beet crops显示文摘 | Gstin F Wauters A 1994 | 1994 | Bull Environ Contam Toxicol1994，53，： | 1 |
| 17 | Imidacloprid insecticide soil metabolism in sugar beet field crops 显示文摘 | Gstin F Wauters A 1996 | 1996 | Bull Environ Contam Toxicol1996，56，： | 1 |
| 18 | Leptin levels in type 2 diabetes:Associations with measures of insulin resistance and insulin secretion显示文摘 | Wauters M Considine RV Yudkin JS | 2003 | Horm Metab Res2003，35，2： | 1 |
| 19 | Thermodynamic analysis of reciprocating compressors显示文摘 | STOUFFS P TAZEROUT M WAUTERS P | 2001 | International Journal of Thermal Sciences2001，40，1： | 1 |
| 20 | Foreign materialin postoperative adhesions显示文摘 | Luijendijk R de Lange D Wauters C | 1996 | Ann Surg1996，223，3： | 1 |
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