|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release显示文摘Sequential administration and controlled release of different drugs are of vital importance for regulating cellular behaviors and tissue regeneration,which usually demands appropriate carriers like microspheres(MS)to control drugs releases.Electrospray has been proven an effective technique to prepare MS with uniform particle size and high drug-loading rate.In this study,we applied electrospray to simply and hierarchically fabricate sphere-in-sphere composite microspheres,with smaller poly(lactic-co-glycolic acid)MS(~8-10 lm in diameter)embedded in a larger chitosan MS(~250-300 lm in diameter).The scanning electron microscopy images revealed highly uniform MS that can be accurately controlled by adjusting the nozzle diameter or voltage.Two kinds of model drugs,bovine serum albumin and chlorhexidine acetate,were encapsulated in the microspheres.The fluorescence-labeled rhodamine-fluoresceine isothiocyanate(Rho-FITC)and ultraviolet(UV)spectrophotometry results suggested that loaded drugs got excellent distribution in microspheres,as well as sustained,slow release in vitro.In addition,far-UV circular dichroism and matrixassisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)results indicated original secondary structure and molecular weight of drugs after electrospraying.Generally speaking,our research proposed a modified hierarchically electrospraying technique to prepare sphere-in-sphere composite MS with two different drugs loaded,which could be applied in sequential,multi-modality therapy. | Zhu Liu Weilong Ye Jingchuan Zheng Qindong Wang Guowu Ma Huiying Liu Xiumei Wang | 2020 | Regenerative Biomaterials2020，7，4： | 5 |
| 2 | One-pot synthesis of the highly efficient bifunctional Ni-SAPO-11 catalyst显示文摘Hydro isomerization of linear alkanes to branched isomers is an important petrochemical process for production of gasoline with high octane number.Non-noble metal bifunctional catalysts used in this process always suffer from low metal dispersion and poor metal-acid synergy.Herein,a facile one-pot synthesis method was used to simultaneously regulate metal particle sizes and acidity of the Ni-SAPO-11 hydroisomerization catalyst.The physicochemical properties are investigated using XANES,EXAFS,TEM/STEM,FT-IR,XPS,UV-vis and NH_3-TPD.Apart from the highly dispersed nickel nanoparticles with an average diameter of 8 nm,the framework Ni~(2+)ions are generated via substituting framework Al~(3+)ions of the SAPO-11.The formed NiP-OH structures have lower deprotonation energy(DPE)than the SiAl-OH ones,contributing more and stronger acid sites to the Ni-SAPO-11 catalyst.The great metal-acid synergy including high metal to acid sites ratio(n_(Ni)/n_A)and close intimacy is obtained for the Ni-SAPO-11 catalyst.The Ni-SAPO-11 catalyst outperforms the counterpart prepared by the impregnation method and exhibits comparable activity and isomers selectivity to the Pt/SAPO-11 catalyst in the n-hexane hydroisomerization. | Yuchao Lyu Weilong Zhan Zhumo Yu Xinmei Liu Ye Yang Xiaoxing Wang Chunshan Song Zifeng Yan | 2021 | Journal of Materials Science & Technology2021，，17： | 4 |
| 3 | Corrigendum to:Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release显示文摘 | Zhu Liu Weilong Ye Jingchuan Zheng Qindong Wang Guowu Ma Huiying Liu Xiumei Wang | 2020 | Regenerative Biomaterials2020，7，4： | 1 |
| 4 | Crosstalk between PC 12 cells and endothelial cells in an artificial neurovascular niche constructed by a dual-functionalized selfassembling peptide nanofiber hydrogel显示文摘The coordination between neurogenesis and angiogenesis plays an important role in nerve tissue development and regeneration.Recently,using bioactive materials to drive neurogenic and angiogenic responses has gained increasing attention.Understanding the neurovascular link between regulatory cues offers valuable insight into the mechanisms underlying nerve regeneration and the design of new bioactive materials.In this study,we utilized a dual-functionalized peptide nanofiber hydrogel presenting the brain-derived neurotrophic factor and vascular endothelial growth factor mimetic peptides RGIDKRHWNSQ(RGI)and KLTWQELYQLKYKGI(KLT)to construct an artificial neurovascular microenvironment.The dual-functionalized peptide nanofiber hydrogel enhanced the neurite outgrowth of pheochromocytoma(PC12)cells and tube-like structures formation of human umbilical vein endothelial cells(HUVECs)in vitro,and promoted rapid lesion infiltration of neural and vascular cells in a rat brain injury model.Using indirect co-culture models,we found that the dual-functionalized peptide hydrogel effectively mediated neurovascular crosstalk by regulating secretion of paracrine factors from PC12 cells and HUVECs.When the two cells types were directly co-cultured on the dua卜functionalized peptide hydrogel,the efficiency of cell-cell communication was enhanced,which further accelerated the differentiation and maturation of PC12 cells with an increased number of pseudopodia and spread morphology,and HUVECs tube-like structure formation.In summary,the dual-functionalized peptide nanofiber hydrogel successfully formed an artificial neurovascular niche to directly regulate the behaviors of neural and vascular cells and promote their neurovascular crosstalk through paracrine signaling and direct cell-cell contact. | Zhe Zhang Yi Chai He Zhao Shuhui Yang Wei Liu Zihui Yang Weilong Ye Chenlong Wang Xiaohan Gao Xiangdong Kong Xiaodan Sun Lingyun Zhao Tuoyu Chen Yuqi Zhang Jiaju Lu Xiumei Wang | 2022 | Nano Research2022，15，2： | 0 |
| 5 | MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1显示文摘Mesenchymal stem cell(MSC)-based therapy has emerged as a promising treatment for spinal cord injury(SCI),but improving the neurogenic potential of MSCs remains a challenge.Mixed lineage leukemia 1(MLL1),an H3K4me3 methyltransferases,plays a critical role in regulating lineage-specific gene expression and influences neurogenesis.In this study,we investigated the role and mechanism of MLL1 in the neurogenesis of stem cells from apical papilla(SCAPs).We examined the expression of neural markers,and the nerve repair and regeneration ability of SCAPs using dynamic changes in neuron-like cells,immunofluorescence staining,and a SCI model.We employed a coimmunoprecipitation(Co-IP)assay,real-time RT-PCR,microarray analysis,and chromatin immunoprecipitation(ChIP)assay to investigate the molecular mechanism.The results showed that MLL1 knock-down increased the expression of neural markers,including neurogenic differentiation factor(NeuroD),neural cell adhesion molecule(NCAM),tyrosine hydroxylase(TH),βIII-tubulin and Nestin,and promoted neuron-like cell formation in SCAPs.In vivo,a transplantation experiment showed that depletion of MLL 1 in SCAPs can restore motor function in a rat SCI model.MLL1 can combine with WD repeat domain 5(WDR5)and WDR5 inhibit the expression of neural markers in SCAPs.MLL1 regulates Hairy and enhancer of split 1(HES1)expression by directly binds to HES1 promoters via regulating H3K4me3 methylation by interacting with WDR5.Additionally,HES1 enhances the expression of neural markers in SCAPs.Our findings demonstrate that MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1.These results provide a potential therapeutic target for promoting the recovery of motor function in SCI patients. | Chen Zhang Weilong Ye Mengyao Zhao Lujue Long Dengsheng Xia Zhipeng Fan | 2023 | International Journal of Oral Science2023，15，4： | 0 |
| 6 | Detrital Zircon U-Pb Dating of Nanshuangyashan Formation in the Jiamusi Massif, NE China and its Tectonic Implications显示文摘A suite of the fossil-rich marine-land interbedded strata(Nanshuangyashan Formation) is distributed at the eastern margin of the Jiamusi massif in the eastern Heilongjiang Province, NE China. The authors had recently discovered a suite of arkose beneath the marine-land interbedded strata, which overlays unconformably on the Permain granite in the eastern margin of the Jiamusi massif. The LA-ICP-MS zircon U-Pb dating indicate that all detrital zircons from the analysed four arkose samples show the four population ages of >800 Ma, 538-481 Ma, 269-250 Ma and 223-215 Ma. The former three population ages are widely recorded in the Jiamusi-Khanka massif and the Songnen massif. The later group is the minimal age population in the analyzed samples, limiting the sedimentation time of the arkoses occurred after the Late Triassic. At present, the minimal age population is not recorded in the Jiamusi massif, but the granites with the ages of 228-210 Ma are widely distributed in the Songnen-Zhangguangcai Range massif and the Khanka massif. The predominantly Permian zircons are characterized by oscillatory zoning and euhedral shapes, with variable zircon εHf(t) values(-5.5 to +11.2), indicating that they were derived from mixture sources, possibly mixed with components of the Songnen-Zhangguangcai Range massif and the Jiamusi-Khanka massif. These results, combined with regional analyses, indicate that the closing of Mudanjiang ocean and Panthalassa ocean possibly existed from Early Permian to Late Triassic. | CUI Weilong ZENG Zhen ZHANG Xingzhou LIU Zhenghong WANG Shijie PU Jianbin FU Qiulin GUO Ye | 2019 | Acta Geologica Sinica(English Edition)2019，93，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员