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| 1 | Recovery of phosphorus as struvite from sewage sludge ash显示文摘Phosphorus(P) is an element vital for all living organisms,yet the world's reserves of phosphate rock are becoming depleted.This study investigated an effective P recovery method from sludge ash via struvite precipitation.Results showed that more than 95% of the total P content was extracted from sludge ash by applying 0.5 mol/L HCl at a liquid/solid ratio of 50 mL/g.Although heavy metal leaching also occurred during P extraction,cation exchange resin efficiently removed the heavy metals from the P-rich solution.Orthogonal tests showed that the optimal parameters for P precipitation as struvite would be a Mg:N:P molar ratio of 1.6:1.6:1 at pH 10.0.X-ray diffraction analysis validated the formation of struvite.Further investigations revealed that the harvested precipitate had a high struvite content(97%),high P bioavailability(94%),and low heavy metal content,which could be considered a high quality fertilizer. | Huacheng Xu Pinjing He Weimei Gu Guanzhao Wang Liming Shao | 2012 | Journal of Environmental Sciences2012，24，8： | 15 |
| 2 | Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress显示文摘In the present study,we aimed to investigate the role of endoplasmic reticulum stress(ERS)and its related inflammation and angiogenesis in liver fibrosis in a rat model of combined hypoxia and nonalcoholic steatohepatitis(NASH)and to confirm whether the intervention of hypoxia-inducible factor 1α(HIF1α)can improve fibrosis.Liver histological changes and biochemical indices,HIF1α,inflammatory factors,ERS-related parameters(GRP78,CHOP,caspase-3,and caspase-12),and angiogenesis indices(VEGFA,VEGFR2,and CD34)were evaluated.Compared with the control rats,the liver tissue of rats with hypoxia and NASH had obvious NASH characteristics and hepatic fibrosis was significantly aggravated,including bridging fibrosis in some rats.The mRNA expression levels of HIF1α,VEGFA,and VEGFR2 and total immunohistochemical staining scores of VEGFR2 and CD34 were significantly increased.In addition,HIF1α silencing significantly decreased HIF1α,biochemical indices(ALT,AST,and TG),inflammatory factors(TNFα,IL6,and IL1β),and angiogenesis indices(CD34 and VEGFR2),consequently,improved the hepatic fibrosis score in the rat model of combined hypoxia and NASH.Taken together,chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and NASH via angiogenesis rather than ERS and HIF1α intervention can improve liver fibrosis,angiogenesis,inflammatory factors,and biochemical indices.Therefore,HIF1α is a key regulatory factor of liver fibrosis in rats with combined hypoxia and NASH. | Wei Wu Weiping Li Jiaojiao Wei Chunsheng Wang Yunliang Yao Weihua Zhu Weimei He Weimei Zhou Jiang Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 8 |
| 3 | Chronic intermittent hypoxia promotes the development of experimental non-alcoholic steatohepatitis by modulating Treg/Th17 differentiation显示文摘The present study aims to characterize the effect of chronic intermittent hypoxia and HIF1α on the non-alcoholic steatohepatitis (NASH)process in mice,and to explore the role of the Treg/Th17 balance in the formation of NASH inflammation and fibrosis.To achieve this purpose,simple steatosis was induced in mice by high-fat diet administration.Subsequently,chronic intermittent hypoxia was simulated by intraperitoneally injecting sodium nitrite.The changes of inflammation, fibrosis,and Treg/Th17 balance in the liver were.quantified under chronic intermittent hypoxia condition and after tail vein injection of HIF1α-siRNA.In addition,T cells were cultured in vitro, and HIF1α expression was either blocked or overexpressed under chronic intermittent hypoxia or normal conditions.Then,the changes of Treg/Th17 balance,inflammatory factors,and cell pathways were measured in each group.Our results demonstrated that chronic intermittent hypoxia accelerates the NASH process,while tail vein injection of HIF1α-siRNA improves liver histology and function.Chronic intermittent hypoxia alters the ratio of Th17 and Treg cells through HIF1α and roTOR signaling,and increases the expressions of NF-κB,IL-6,and IL-17,but decreases IL-10 expression.Inhibition of the mTOR-HIF1α-TLR4-IL-6 pathway increases the ratio of Treg/Th17. Thus,chronic intermittent hypoxia modulates the Treg/Th17 balance by inducing HIF1α,resulting in the activation of the mTOR-HIF1α-TLR4-IL-6 pathway,which accelerates the formation of NASH and fibrosis. | Jiang Liu Weiping Li Weihua Zhu Weimei He Hui Zhao Yu Xiang Chunyan Liu Wei Wu | 2018 | Acta Biochimica et Biophysica Sinica2018，50，12： | 4 |
| 4 | Effect of air-flow rate and turning frequency on bio-drying of dewatered sludge 显示文摘 | ZHAO Ling GU Weimei HE Pinjing | 2010 | Water Research2010，44，20： | 1 |
| 5 | Biodegradation potential of bulking agents used in sludge bio-drying and their contribution to bio-genera-ted heat 显示文摘 | ZHAO Ling GU Weimei HE Pinjing | 2011 | Water Research2011，45，6： | 1 |
| 6 | Identification of atypical T4SS effector proteins mediating bacterial defense显示文摘To remain competitive,proteobacteria use various contact-dependent weapon systems to defend against microbial com-petitors.The bacterial-killing type IV secretion system(T4SS)is one such powerful weapon.It commonly controls the killing/competition between species by secreting the lethal T4SS effector(T4E)proteins carrying conserved XVIPCD domains into competing cells.In this study,we sought knowledge to understand whether the bacterial-killing T4SS-producing bacteria encode T4E-like proteins and further explore their biological functions.To achieve this,we designed a T4E-guided approach to discover T4E-like proteins that are designated as atypical T4Es.Initially,this approach required scientists to perform simple BlastP search to identify T4E homologs that lack the XVIPCD domain in the genomes of T4SS-producing bacteria.These homologous genes were then screened in Escherichia coli to identify antibacterial candidates(atypical T4Es)and their neighboring detoxification proteins,followed by testing their gene cotranscription and validating their physical interactions.Using this approach,we did discover two atypical T4E proteins from the plant-beneficial Lysobacter enzymogenes and the phytopathogen Xanthomonas citri.We also provided substantial evidence to show that the atypical T4E protein Le1637-mediated bacterial defense in interspecies interactions between L.enzymogenes and its competitors.Therefore,the newly designed T4E-guided approach holds promise for detecting functional atypical T4E proteins in bacterial cells. | Xi Shen Zixiang Yang Zihan Li Dan Xiong Jinxing Liao Weimei He Danyu Shen Xiaolong Shao Ben Niu Yongxing He Yong-Gui Gao Guoliang Qian | 2023 | mLife2023，2，3： | 0 |
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