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| 1 | Arenobufagin is a novel isoform-specific probe for sensing human sulfotransferase 2A1显示文摘Human cytosolic sulfotransferase 2 A1(SULT2A1) is an important phase II metabolic enzyme. The detection of SULT2A1 is helpful for the functional characterization of SULT2A1 and diagnosis of its related diseases. However, due to the overlapping substrate specificity among members of the sulfotransferase family, it is difficult to develop a probe substrate for selective detection of SULT2A1. In the present study, through characterization of the sulfation of series of bufadienolides, arenobufagin(AB) was proved as a potential probe substrate for SULT2A1 with high sensitivity and specificity. Subsequently, the sulfation of AB was characterized by experimental and molecular docking studies. The sulfate-conjugated metabolite was identified as AB-3-sulfate.The sulfation of AB displayed a high selectivity for SULT2A1 which was confirmed by in vitro reaction phenotyping assays. The sulfation of AB by human liver cytosols and recombinant SULT2A1 both obeyed Michaelis-Menten kinetics, with similar kinetic parameters. Molecular docking was performed to understand the interaction between AB and SULT2A1, in which the lack of interaction with Met-137 and Tyr-238 of SULT2A1 made it possible to eliminate substrate inhibition of AB sulfation. Finally, the probe was successfully used to determine the activity of SULT2A1 and its isoenzymes in tissue preparations of human and laboratory animals. | Xiangge Tian Chao Wang Peipei Dong Yue An Xinyu Zhao Weiru Jiang Gang Wang Jie Hou Lei Feng Yan Wang Guangbo Ge Xiaokui Huo Jing Ning Xiaochi Ma | 2018 | Acta Pharmaceutica Sinica B2018，8，5： | 2 |
| 2 | Genome-Wide Analysis of the AP2/ERF Gene Family in Prunus mume显示文摘 | Dongliang Du Ruijie Hao Tangren Cheng Huitang Pan Weiru Yang Jia Wang Qixiang Zhang | 2013 | Plant Molecular Biology Reporter2013，，3： | 1 |
| 3 | Photoinduced bendingbehavior of cross-linked azobenzene liquid-crystalline polymerfilms witha poly(oxyethylene) backbone显示文摘 | Lv Jiuan Wang Weiru Xu Jixiang | 2014 | Macromol RapidCommun2014，35，12： | 1 |
| 4 | Si3N4 BN SiO2 based microwave transparent materials显示文摘 | Zhang Weiru Wang Chonghai Li Ling | 2008 | Key Engineering Materials2008，68，： | 1 |
| 5 | An Attribute-Based Signcryption Scheme and Its Application in Information Hiding显示文摘In order to provide a secure,reliable and flexible way to hide information,a new attribute-based signcryption scheme based on ciphertext-policy and its security proof are presented.This scheme not only can simultaneously fulfil both authentication and confidentiality in an efficient way,but also implements a hierarchical decryption in one group and also between different groups according to user's authority(different users satisfying the same access structure can be considered as a group).We provide a solution to information hiding using our proposed scheme which can embed ciphertext into a carrier.Because the hierarchical decryption property,different users will obtain different message from the same carrier.Illegal user can not get any information without private key because message existed in the carrier is ciphertext.Such solution can be applied in sharing important message under the public network. | PENG Changgen WANG Weiru TIAN Youliang YUAN Zhilong | 2016 | Chinese Journal of Electronics2016，25，4： | 1 |
| 6 | Is firm-specific innovation associated with greater value appropriation?The roles of environmental dynamism and technological diversity显示文摘 | WANG Heli CHEN Weiru | 2010 | Research Policy2010，39，1： | 1 |
| 7 | Systematic analysis of artificial intelligence in the era of industry 4.0显示文摘Artificial Intelligence has been playing a profound role in the global economy,social progress,and people’s daily life.With the increasing capabilities and accuracy of AI,the application of AI will have more impacts on manufacturing and service areas in the era of industry 4.0.This study conducts a systematic literature review to study the state-of-the-art on AI in industry 4.0.This paper describes the development of industries and the evolution of AI.This paper also identifies that the development and application of AI will bring not only opportunities but also challenges to industry 4.0.The findings provide a valuable reference for researchers and practitioners through a multi-angle systematic analysis of AI.In the era of industry 4.0,AI system will become an innovative and revolutionary assistance to the whole industry. | Weiru Chen Wu He Jiayue Shen Xin Tian Xianping Wang | 2023 | Journal of Management Analytics2023，10，1： | 0 |
| 8 | DeepOCL:A deep neural network for Object Constraint Language generation from unrestricted nature language显示文摘Object Constraint Language(OCL)is one kind of lightweight formal specification,which is widely used for software verification and validation in NASA and Object Management Group projects.Although OCL provides a simple expressive syntax,it is hard for the developers to write correctly due to lacking knowledge of the mathematical foundations of the first-order logic,which is approximately half accurate at the first stage of devel-opment.A deep neural network named DeepOCL is proposed,which takes the unre-stricted natural language as inputs and automatically outputs the best-scored OCL candidates without requiring a domain conceptual model that is compulsively required in existing rule-based generation approaches.To demonstrate the validity of our proposed approach,ablation experiments were conducted on a new sentence-aligned dataset named OCLPairs.The experiments show that the proposed DeepOCL can achieve state of the art for OCL statement generation,scored 74.30 on BLEU,and greatly outperformed experienced developers by 35.19%.The proposed approach is the first deep learning approach to generate the OCL expression from the natural language.It can be further developed as a CASE tool for the software industry. | Yilong Yang Yibo Liu Tianshu Bao Weiru Wang Nan Niu Yongfeng Yin | 2024 | CAAI Transactions on Intelligence Technology2024，9，1： | 0 |
| 9 | BULKED SEGREGANT RNA SEQUENCING(BSR-SEQ)IDENTIFIES A NOVEL ALLELE ASSOCIATED WITH WEEPING TRAITS IN PRUNUS MUME显示文摘Weeping species are used both as ornamental plants and for breeding dwarf plant types.However,exploration of casual genes controlling weeping traits is rather limited.Here,we identified individuals with contrasting phenotypes from an Fx bi-parental mapping population of Prunus mume which was developed from a cross between the upright cultivar/Liuban,and the weeping cultivar'Fentai Chuizhi'.Bulked segregant RNA sequencing was used and five QTLs on chromosome 7 were identified.The Pm024074(PmilGT72B3)allele,belonging to the UDP-glycosyltransferase superfamily containing the coniferyl-alcohol glucosyltransferase domain,was identified in a genomic region overlapping with a previously identified QTL;and had a synonymous transition of T66(upright)to C(weeping)in the coding sequence and a 470-bp deletion in the promoter region.Pm024074 had exceptionally high expression in buds and stems of weeping P.mume.Weighted correlation network analysis indicates that genes neighboring Pm024074 were significantly associated with plant architecture.In addition,a reliable single nucleotide polymorphism marker was developed based on the variation in the Pm024074 gene,providing precise marker-assisted breeding for weeping traits.This study provides insights into the genetic mechanism governing the weeping trait in P.mume,and indicates potential applications for the manipulation of tree architecture. | Xiaokang ZHUO Tangchun ZHENG Zhiyong ZHANG Suzhen LI Yichi ZHANG Lidan SUN Weiru YANG Jia WANG Tangren CHENG Qixiang ZHANG | 2021 | Frontiers of Agricultural Science and Engineering2021，8，2： | 0 |
| 10 | Preliminary Evaluation of Hemodynamic Effects of Fontan Palliation on Renal Artery Using Computational Fluid Dynamics显示文摘Background:The assessment of renal function is important to the prognosis of patients needing Fontan palliation due to the reconstructed compromised circulation.To know the relationship between the kidney perfusion and hemodynamic characteristics during surgical design could reduce the risk of acute kidney injury(AKI)and the postoperative complications.However,the issue is still unsolved because the current clinical evaluation methods are unable to predict the hemodynamic changes in renal artery(RA).Methods:We reconstructed a three-dimensional(3D)vascular model of a patient requiring Fontan palliation.The technique of computational fluid dynamics(CFD)was utilized to explore the changes of RA hemodynamics under different possible blood flow rates.The relationship between the kidney perfusion and hemodynamic characteristics was investigated.Results:The calculated results indicated the declined tendency of the pressure and pressure drop as the flow rate decreased.When the flow rate decreased to two-thirds of its baseline,both the pressure of left renal artery(LRA)and the pressure of right renal artery(RRA)dipped below 50%,and the pressure of RRA fell more quickly than that of LRA.Uneven distribution of WSS was observed on the trunk of RA,and the lowest WSS was found at the distal of RA.The average WSS in RA dropped to around 50%as the flow rate reached one-third of its baseline.Conclusions:As a promising approach,CFD can be utilized to quantitatively evaluate the hemodynamic characteristics of RA and contribute to offsetting the drawbacks of clinical assessments of renal function,to help realize better prognosis for the patients with Fontan palliation. | Jinlong Liu Jing Shi Weiru Luo Zhirong Tong Lefei Yang Peixuan Sun Tianyi Li Jun Du Qian Wang | 2023 | Congenital Heart Disease2023，18，1： | 0 |
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