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| 1 | PTPN12与非小细胞肺癌放射敏感性的关系研究显示文摘目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中非受体型蛋白酪氨酸磷酸酶12(protein tyrosine phosphatase non-receptor type 12,PTPN12)表达与放疗疗效的关系,评估PTPN12基因下调与H1299细胞放射敏感性的关系。方法:回顾性分析2013年9月至2014年10月河北医科大学第四医院92例接受根治性放疗的NSCLC患者的临床资料,通过免疫组织化学方法检测肿瘤组织中PTPN12的表达,并分析其与患者放疗疗效的关系。通过shRNA-PTPN12干扰质粒转染H1299细胞,利用实时定量PCR技术及Western blot技术检测PTPN12在mRNA及蛋白水平的表达。通过克隆形成实验构建细胞生存曲线,评估PTPN12基因下调对H1299细胞放射敏感性的作用。结果:PTPN12低表达患者的放疗疗效明显优于高表达者(80.0%vs.57.1%,P=0.018)。多因素分析显示PTPN12表达是影响放疗疗效的唯一因素。构建shRNA-GFP及shRNA-PTPN12干扰质粒转染H1299细胞,获得稳定转染细胞株H1299-shGFP及H1299-shPTPN12。细胞生存曲线显示H1299-shGFP及H1299-shPTPN12细胞的D0、Dq、SF2值分别为3.02 Gy、3.41 Gy、0.94及1.81 Gy、2.40 Gy、0.72,具有显著性差异(P<0.05)。结论:PTPN12低表达与NSCLC患者的放疗疗效密切相关,而PTPN12基因下调能明显增加H1299细胞的放射敏感性。 | 胡文霞 刘志坤 张魏丽 Scott Welford 苏景伟 | 2016 | 中国肿瘤临床2016，43，7： | 4 |
| 2 | Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization显示文摘 | Welford SM Gregg J Chen E | | 0，，： | 2 |
| 3 | Mechanistic studies on three 2 - oxoglutarate - dependent oxygenases of flavonoid biosynthesis : anthocyanidin synthase, flavonol synthase, and flavanone 3b - hydroxylase 显示文摘 | Tumbull J J Nakajima J I Welford R W D | 2004 | Journal of Biological Chemistry2004，279，： | 1 |
| 4 | Extended producer respon- sibility and Eco-design changes: Perspectives from China 显示文摘 | YU J HILLS P WELFORD R | 2008 | Wiley InterSeienee2008，，15： | 1 |
| 5 | Detection of differentially expressed genes in primary tumor using representational differences analysis coupled to microarray hybridzation 显示文摘 | Welford SM Gregg J Chen E | 1998 | Nucleic Acid Research1998，26，12： | 1 |
| 6 | TIE2-expressing macrophages limit the therapeutic efficacy of the vascular- disrupting agent combretastatin A4 phosphate in mice显示文摘 | Welford AF Biziato D Coffeh SB | 1969 | J Clin Invest1969，121，： | 1 |
| 7 | Use of annular apertures to increase focal depth 显示文摘 | WELFORD W | 1960 | Journal of the Optical Society of America1960，50，8： | 1 |
| 8 | Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis-anthocyanidin syn- thase, flavonol synthase, and flavanone 3 beta-hydroxy- lase显示文摘 | Turnbull J J Nakajima J Welford R W D | 2004 | Journal of Biological Chemistry2004，279，2： | 1 |
| 9 | Structure and mechanism of anthocyanidin systhase from Arabidopis thaliana显示文摘 | Wilmouth PC Tumbull JJ Welford RW | 2002 | Structure2002，10，1： | 1 |
| 10 | TIE2-expressing maerophages limit the therapeutic efficacy of the vascu- lar-disrupting agent combretastatin A4 phosphate in mice 显示文摘 | Welford AF Biziato D Coffelt SB | 2011 | J Clin Invest2011，121，5： | 1 |
| 11 | Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase and flavanone 3β-hydroxylase 显示文摘 | Tumbull J J Nakajima J Welford R W | 2004 | Plant J Biol Chem2004，279，2： | 1 |
| 12 | Globalization, corporate social responsibility and human rights 显示文摘 | Welford R | 2002 | Corporate Social Responsibility and Environmental Management2002，9，1： | 1 |
| 13 | Corporate Social Responsibility in Asian Supply Chains 显示文摘 | Welford R Frost S | 2006 | Corporate Social Responsibility and Environmental Management2006，13，： | 1 |
| 14 | Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization显示文摘 | Welford JG Emily C | 1998 | Nucleic Acids Research1998，26，12： | 1 |
| 15 | Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis:anthocyanidin synthase,flavonol synthase,and flavanone 3beta-hydroxylase显示文摘 | Tumbull J.J Nakajima J Welford R.W Yamazaki M Saito K and Schofield C.J | | 0，，： | 1 |
| 16 | Use of annular apertures to increase focal depth显示文摘 | WELFORD W T | 1960 | JOSA A1960，50，8： | 1 |
| 17 | EWS/FLI1 up regu- lates m E-C, a cyclin-selective ubiquitin conjugating enzyme in- volved in cyclin B estruction 显示文摘 | Arvand A Bastians H Welford SM | 1998 | Oncogene1998，17，16： | 1 |
| 18 | Structureand mechanism of anthocyanidin synthase from Arabidopsis thaliana 显示文摘 | WILMOUTH R C TURNBULL J J WELFORD R W | 2002 | Structure(Camb)2002，10，： | 1 |
| 19 | Corporate Social Responsibility in Europe and Asia显示文摘 | Richard Welford | 2004 | Journal of Corporate Citizenship2004，，13： | 1 |
| 20 | Detection of differentially expressed genes in primal tumor tissues using representational differences analysis coupled to microarray hyhridizationl 显示文摘 | Welford SM Gregg J Chem E | 1998 | Nucleic Acid Res1998，26，12： | 1 |
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