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| 1 | Numerical and experimental studies on solidification of AZ80 magnesium alloy under out-of-phase pulsed magnetic field显示文摘For obtaining the finer grains of magnesium alloy,a novel combined pulsed magnetic field with different initial phases,also called out-ofphase pulsed magnetic field(OPPMF),was applied to study the solidification structure of AZ80 magnesium alloy.The numerical simulation was simultaneously conducted to investigate the refinement mechanisms.The experimental results showed that the macrostructure could be effectively refined by applying external magnetic field.Meanwhile,finer grains were obtained with the higher current intensity.However,the increase of current intensity could only refine the grains to about 0.5 mm.Furthermore,compared to a single pulsed magnetic field(PMF)and alternating series of OPPMF(Connection II),a finer structure was observed when the consecutive series of OPPMF(Connection I)was imposed.In contrast with a single PMF and Connection II,the numerical results showed that the greater axial Lorentz force was obtained under the Connection I,generating the stronger forced flow in the melt.It is believed that abundant nuclei could detach from the mold wall and move faster into the interior melt due to the stronger forced flow;besides,the lower superheat and greater temperature uniformity in bulk melt were realized,accounting for the finest structures under the Connection I. | Wenchao Duan Siqi Yin Wenhong Liu Zhong Zhao Kun Hu Ping Wang Jianzhong Cui Zhiqiang Zhang | 2021 | Journal of Magnesium and Alloys2021，9，1： | 5 |
| 2 | A cryptic inhibitor of cytokinin phosphorelay controls rice grain size显示文摘Plant hormone cytokinin signals through histidine-aspartic acid(H-D)phosphorelay to regulate plant growth and development.While it is well known that the phosphorelay involves histidine kinases,histidine phosphotransfer proteins(HPs),and response regulators(RRs),how this process is regulated by external components remains unknown.Here we demonstrate that protein phosphatase with kelch-like domains(PPKL1),known as a signaling component of steroid hormone brassinosteroid,is actually a cryptic inhibitor of cytokinin phosphorelay in rice(Oryza sativa).Mutation at a specific amino acid D364 of PPKL1 activates cytokinin response and thus enlarges grain size in a semi-dominant mutant named s48.Overexpression of PPKL1 containing D364,either with the deletion of the phosphatase domain or not,rescues the s48 mutant phenotype.PPKL1 interacts with OsAHP2,one of authentic HPs,and D364 resides in a region resembling the receiver domain of RRs.Accordingly,PPKL1 can utilize D364 to suppress OsAHP2-to-RR phosphorelay,whereas mutation of D364 abolishes the effect.This function of PPKL1 is independent of the phosphatase domain that is required for brassinosteroid signaling.Importantly,editing of the D364-residential region produces a diversity of semi-dominant mutations associated with variously increased grain sizes.Further screening of the edited plants enables the identification of two genotypes that confer significantly improved grain yield.Collectively,our study uncovers a noncanonical cytokinin signaling suppressor and provides a robust tool for seed rational design. | Dapu Liu He Zhao Yunhua Xiao Guoxia Zhang Shouyun Cao Wenchao Yin Yangwen Qian Yanhai Yin Jinsong Zhang Shouyi Chen Chengcai Chu Hongning Tong | 2022 | Molecular Plant2022，15，2： | 3 |
| 3 | A cynomolgus monkey with naturally occurring Parkinson’s disease显示文摘Evidence for the existence of monkeys with spontaneous Parkinson’s disease(PD)has been lacking.Here,we screened macaque colonies at the Kunming Primate Research Center(Association for Assessment and Accreditation of Laboratory Animal Care(AAALAC)accredited),Kunming Institute of Zoology,Chinese Academy of Sciences,which has a large population of monkeys(>2400),to identify naturally occurring PD monkeys. | Hao Li Ling-Yan Su Lixin Yang Min Li Qianjin Liu Zhenhui Li Yan Hu Hongwei Li Shihao Wu Wenchao Wang Yingzhou Hu Zhengbo Wang Joshua DRizak Baihui Huang Min Xu Jing Wu Long-Bao Lv Christoph W.Turck Yong Yin Yong-Gang Yao Bing Su Xintian Hu | 2021 | National Science Review2021，8，3： | 3 |
| 4 | The U-box ubiquitin ligase TUD1 promotes brassinosteroid-induced GSK2 degradation in rice显示文摘Brassinosteroids(BRs)are a class of steroid hormones with great potential for use in crop improvement.De-repression is usually one of the key events in hormone signaling.However,how the stability of GSK2,the central negative regulator of BR signaling in rice(Oryza sativa),is regulated by BRs remains elusive.Here,we identify the U-box ubiquitin ligase TUD1 as a GSK2-interacting protein by yeast two-hybrid screening.We show that TUD1 is able to directly interact with GSK2 and ubiquitinate the protein.Phenotypes of the tud1 mutant are highly similar to those of plants with constitutively activated GSK2.Consistent with this finding,GSK2 protein accumulates in the tud1 mutant compared with the wild type.In addition,inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability.By contrast,BRs can induce GSK2 degradation but promote TUD1 accumulation.Furthermore,the GSK2 degradation process is largely impaired in tud1 in response to BR.In conclusion,our study demonstrates the role of TUD1 in BR-induced GSK2 degradation,thereby advancing our understanding of a critical step in the BR signaling pathway of rice. | Dapu Liu Xiaoxing Zhang Qingliang Li Yunhua Xiao Guoxia Zhang Wenchao Yin Mei Niu Wenjing Meng Nana Dong Jihong Liu Yanzhao Yang Qi Xie Chengcai Chu Hongning Tong | 2023 | Plant Communications2023，4，2： | 2 |
| 5 | Co-editing PINK1 and DJ-1 Genes Via Adeno-Associated Virus-Delivered CRISPR/Cas9 System in Adult Monkey Brain Elicits Classical Parkinsonian Phenotype显示文摘Whether direct manipulation of Parkinson’s disease(PD)risk genes in the adult monkey brain can elicit a Parkinsonian phenotype remains an unsolved issue.Here,we used an adeno-associated virus serotype 9(AAV9)-delivered CRISPR/Cas9 system to directly co-edit PINK1 and DJ-1 genes in the substantia nigras(SNs)of two monkey groups:an old group and a middle-aged group.After the operation,the old group exhibited all the classic PD symptoms,including bradykinesia,tremor,and postural instability,accompanied by key pathological hallmarks of PD,such as severe nigral dopaminergic neuron loss(>64%)and evidentα-synuclein pathology in the gene-edited SN.In contrast,the phenotype of their middle-aged counterparts,which also showed clear PD symptoms and pathological hallmarks,were less severe.In addition to the higher final total PD scores and more severe pathological changes,the old group were also more susceptible to gene editing by showing a faster process of PD progression.These results suggested that both genetic and aging factors played important roles in the development of PD in the monkeys.Taken together,this system can effectively develop a large number of genetically-edited PD monkeys in a short time(6–10 months),and thus provides a practical transgenic monkey model for future PD studies. | Hao Li Shihao Wu Xia Ma Xiao Li Tianlin Cheng Zhifang Chen Jing Wu Longbao Lv Ling Li Liqi Xu Wenchao Wang Yingzhou Hu Haisong Jiang Yong Yin Zilong Qiu Xintian Hu | 2021 | Neuroscience Bulletin2021，37，9： | 2 |
| 6 | Brassinosteroid-regulated plant growth and development and gene expression in soybean显示文摘Brassinosteroids(BRs) are endogenous phytohormones that play important roles in regulating plant growth and development.In this study, we evaluated the effects of brassinolide(BL, one of the active BRs) on soybean and identified roles of the hormone in regulating multiple aspects of plant growth and development.BL application promoted hypocotyl and epicotyl elongation in the light but blocked epicotyl elongation in the dark.High levels of castasterone and BL accumulated in light-grown plants.BL disrupted shoot negative gravitropism, whereas gibberellin did not.BL delayed leaf senescence.Transcriptome analysis showed that BL induced cell wall-modifying genes and auxin-associated genes but suppressed a class of WRKY genes involved in senescence and stress responses,showing the complex roles of BRs in multiple biological processes. | Wenchao Yin Nana Dong Mei Niu Xiaoxing Zhang Lulu Li Jun Liu Bin Liu Hongning Tong | 2019 | The Crop Journal2019，7，3： | 2 |
| 7 | Soil physical properties,nutrients,and crop yield with two-year tillage rotations under a winter wheat-summer maize double cropping system显示文摘Winter wheat and summer maize were planted from 2015-2017 to study the effects of different rotational tillage patterns on soil physicochemical properties,crop yield,water content,and fertilizer utilization.The tillage treatments were designed as wheat subsoiling-maize no tillage(WS-MN),wheat rotary tillage-maize subsoiling(WR-MS),wheat subsoiling-maize subsoiling(WS-MS),and conventional wheat rotary tillage-maize no tillage(WR-MN)as a control.Among the four treatments,WS-MN and WR-MS were single-season subsoiling treatments,and WS-MS was a two-season subsoiling treatment.The average soil bulk density decreased by 7.6%in the single-and double-season subsoiling groups compared to the WR-MN group,and the total porosity and noncapillary porosity increased by 10.7%and 12.2%,respectively.Single-or double-season subsoiling treatment was not conducive to water storage in the 0-20 cm soil layer but increased the water content of the 20-140 cm soil layer,and the average soil water content of the 0-140 cm layer was increased by 11.6%in the two-growing season treatment groups compared with the WR-MN group.In WS-MS and WS-MN groups compared with the WR-MN group,the soil ammonium nitrogen content was increased by an average of 18.6%in 0-20 cm soil and 16.8%in 20-100 cm soil;soil nitrate-nitrogen content was decreased by 13.5%in 0-100 cm soil;total organic carbon and microbial carbon contents in the 15-30 cm soil were increased by 18.1%and 12.7%,respectively;and soil urease,catalase,and alkaline phosphatase activities were increased by 46.1%,15.2%,and 23.1%,respectively.Annual crop yield and water use efficiency increased by 8.9%and 15.0%,respectively,in both the single-and double-season subsoiling treatment groups.This study demonstrated the advantages of subsoiling tillage and suggested that it is suitable for crop cultivation in the Haihe Plain,China. | Baozhong Yin Pan Liu Yongwei Cui Zhaohui Hu Xiangling Li Zhihua Pan Wenchao Zhen | 2022 | International Journal of Agricultural and Biological Engineering2022，15，1： | 2 |
| 8 | Freeze-drying and hot-pressing strategy to embed two-dimensional Ti_(0.87)O_(2) monolayers in commercial polypropylene films with enhanced dielectric properties显示文摘The dielectric capacitor has been widely used in advanced electronic and electrical power systems due to its capability of ultrafast charging–discharging and ultrahigh power density.Nevertheless,its energy density is still limited by the low dielectric constant(≈2.2)of the commercial dielectric polypropylene(PP).The conventional enhancement strategy by embedding inorganic fillers in PP matrix is still difficult and challenging due to that PP hardly dissolves in any inorganic/organic solvent.In this work,we develop a new strategy including freeze-drying,surface functionalization,and hot-pressing to incorporate Ti_(0.87)O_(2) monolayers in PP film.A series of uniform composited Ti_(0.87)O_(2)@PP film has been successfully fabricated with Ti0.87O2 content range of 0–15 wt%.The maximum dielectric constant of the as-prepared Ti_(0.87)O_(2)@PP film is 3.27 when the Ti_(0.87)O_(2) content is 9 wt%,which is about 1.5 times higher than that of pure PP.Our study provides a feasible strategy to embed two-dimensional material into commercial PP thin-film with superior dielectric performance for practical application. | Wenchao Tian Fei Yan Cailing Cai Zeyi Wu Chenchen Zhang Ting Yin Sijia Lao Linfeng Hu | 2021 | Journal of Advanced Ceramics2021，10，2： | 1 |
| 9 | Coordinated effects of root autotoxic substances and Fusarium oxysporum Schl. f. sp. fragariae on the growth and replant disease of strawberry显示文摘 | Xusheng Zhao Wenchao Zhen Yongzhi Qi Xuejing Liu Baozhong Yin | 2009 | Frontiers of Agriculture in China2009，，1： | 1 |
| 10 | Coordinated effects of root autotoxic substances and Fusarium oxysporum Schl. f. sp. fragariae on the growth and replant disease of strawberry显示文摘 | Xusheng Zhao Wenchao Zhen Yongzhi Qi Xuejing Liu Baozhong Yin | 2009 | Frontiers of Agriculture in China2009，，1： | 1 |
| 11 | A simple solvothermal route to synthesize graphene-modified LiFePO 4 cathode for high power lithium ion batteries显示文摘 | Yin Zhang Wenchao Wang Penghui Li Yanbao Fu Xiaohua Ma | 2012 | Journal of Power Sources2012，，： | 1 |
| 12 | The divergence of brassinosteroid sensitivity between rice subspecies involves natural variation conferring altered internal auto-binding of OsBSK2显示文摘Japonica/geng and indica/xian are two major rice(Oryza sativa)subspecies with multiple divergent traits,but how these traits are related and interact within each subspecies remains elusive.Brassinosteroids(BRs)are a class of steroid phytohormones that modulate many important agronomic traits in rice.Here,using different physiological assays,we revealed that japonica rice exhibits an overall lower BR sensitivity than indica.Extensive screening of BR signaling genes led to the identification of a set of genes distributed throughout the primary BR signaling pathway with divergent polymorphisms.Among these,we demonstrate that the C38/T variant in BR Signaling Kinase2(OsBSK2),causing the amino acid change P13L,plays a central role in mediating differential BR signaling in japonica and indica rice.OsBSK2in indica plays a greater role in BR signaling than OsB SK2in japonica by affecting the auto-binding and protein accumulation of OsBSK2.Finally,we determined that OsBSK2 is involved in a number of divergent traits in japonica relative to indica rice,including grain shape,tiller number,cold adaptation,and nitrogen-use efficiency.Our study suggests that the natural variation in OsB SK2 plays a key role in the divergence of BR signaling,which underlies multiple divergent traits between japonica and indica. | Wenchao Yin Lulu Li Zhikun Yu Fan Zhang Dapu Liu Hongkai Wu Mei Niu Wenjing Meng Xiaoxing Zhang Nana Dong Yanzhao Yang Jihong Liu Yongqiang Liu Guoxia Zhang Jianlong Xu Shimei Wang Chengcai Chu Qian Qian Hongning Tong | 2022 | Journal of Integrative Plant Biology2022，64，8： | 0 |
| 13 | POLLEN STERILITY,a novel suppressor of cell division,is required for timely tapetal programmed cell death in rice显示文摘Timely programmed cell death(PCD) of the tapetum supplying nutrients to microspores is a prerequisite for normal pollen development.Here we identified a unique mutant of rice(Oryza sativa L.),pollen sterility(post),which showed aborted pollens accompanied with extra-large husks.Due to failure of timely PCD of tapetal cells,post exhibited abnormal pollen wall patterning and defective pollen grains.By map-based cloning,we identified a causal gene,POST,encoding a novel protein which is ubiquitously localized in cells.RNA in situ hybridization showed that POST is highly detected in the tapetum and microspores at stages 8 and 9.Transcriptome analysis indicated that POST could function as an important regulator of the metabolic process involved in tapetal PCD.Compared with wild-type rice,post mutant has an increased cell number resulting from elevated expression of cell cycle associated genes in grain husks.Overexpression of POST inhibits grain size in wild type,while appropriate expression of POST in post mutant can recover the seed fertility but has little effect on the large grains,illustrating that fine-tuning of POST expression could be a potential strategy for rice yield improvement.The connection between cell division and cell death conferred by POST provides novel insights into the understanding of the tapetal PCD process. | Ronghui Che Bin Hu Wei Wang Yunhua Xiao Dapu Liu Wenchao Yin Hongning Tong Chengcai Chu | 2022 | Science China(Life Sciences)2022，65，6： | 0 |
| 14 | Fully coupled electrothermal simulation of resistive random access memory (RRAM) array显示文摘Dear editor,Resistive random access memory(RRAM)is a promising candidate for next generation memory technology[1]and the rapid progress in the threedimensional(3D)integration technology facilitates the design of highly integrated and miniaturized RRAM devices[2]. | Da-Wei WANG Wen-Sheng ZHAO Wenchao CHEN Hao XIE Wen-Yan YIN | 2020 | Science China(Information Sciences)2020，63，8： | 0 |
| 15 | Elevated Atmospheric CO_(2)Reduced Antibiotics Accumulation in Rice Grains and Soil ARGs Abundance in Multiple Antibiotics-contaminated Paddy Fields显示文摘Antibiotic resistance genes(ARGs)as new pollutants have become a global environmental pollution problem in recent years.Elevated atmospheric CO_(2) is one of the major factors affecting global climate change.But,the impacts of elevated CO_(2) on soil ARGs in multiple antibiotics-contaminated paddy soils are largely unknown.In this study,six antibiotics including sulfadiazine(SDZ),sulfamethoxazole(SMZ),tetracycline(TC),oxytetracycline(OTC),enrofloxacin(ENR),and ciprofloxacin(CIP)were selected to investigate their combined effects on rice biomass,antibiotics accumulation,soil bacterial community and ARGs under elevated CO_(2) levels.Results showed that elevated CO_(2) significantly reduced the accumulation of SMZ,OTC,ENR,and CIP in rice grains by 18.98%,20.07%,41.73%,and 44.25%,respectively.Elevated CO_(2) could affect soil microbialβ-diversity,and tend to reduce the microbial functions of human diseases,organismal systems,and genetic information processing.In addition,elevated CO_(2) significantly decreased the abundance of sulfonamide ARGs,tetracycline ARGs,and quinolone ARGs by 19.59%,18.58%,and 28.96%,respectively,while increased that of multidrug ARGs by 11.54%.Overall,this study emphasized that elevated CO_(2) may mitigate the threat of antibiotics contamination to rice food security but aggravate the environmental risk of multidrug ARGs in soil,contributing to a better understanding of the consequences of elevated CO_(2) levels on food security and soil ecological health in multiple antibiotics-contaminated paddy fields. | WANG Yabo XU Meiling WANG Xiaojie AI Fuxun DU Wenchao YIN Ying JI Rong GUO Hongyan | 2023 | Chemical Research in Chinese Universities2023，39，3： | 0 |
| 16 | Maize centromeres:where sequence meets epigenetics显示文摘The centromere is a highly organized structure mainly composed of repeat sequences,which make this region extremely difficult for sequencing and other analyses.It plays a conserved role in equal division of chromosomes into daughter cells in both mitosis and meiosis.However,centromere sequences show notable plasticity.In a dicentric chromosome,one of the centromeres can become inactivated with the underlying DNA unchanged.Furthermore,formerly inactive centromeres can regain activity under certain conditions.In addition,neocentromeres without centromeric repeats have been found in a wide spectrum of species.This evidence indicates that epigenetic mechanisms together with centromeric sequences are associated with centromere specification. | Wenchao YIN James A.BIRCHLER Fangpu HAN | 2011 | Frontiers in Biology2011，6，2： | 0 |
| 17 | Ecological and Low-carbon Technology in Urban Decentralized Treatment and Reclamation of Wastewater显示文摘China's water shortage situation is grim.Deep exploration of non-traditional water resources and relief of contradiction between China's economic development and water resources shortage is the only way to ensure the sustainable development of China.Urban sewage treatment and reclamation is a more stable and widely used method of non-traditional water resources development and utilization.However,urban centralized reclaimed water treatment faces many problems such as high transportation cost and difficulty in laying reusing pipe network.Relatively speaking,decentralized reclaimed water treatment technology has obvious advantages and is gradually getting more attention.In this research,three types of wastewater treatment process featured with urban decentralized low-carbon ecological technology have been mainly introduced.In addition,the operating data of relevant domestic and foreign engineering cases were analyzed from the perspectives of treatment effect,energy consumption level,applicable scale and occupied area,etc.This study considers that these three new sewage processes based on the principle of ecological engineering have the advantages of compact structure,small footprint,low water consumption,stable effluent quality,beautiful appearance and large applicable scale,and are worthy of researching and importing. | YIN Wenchao LIU Yongwang YI Wenting LU Xingchao ZHAO Xin ZHAO Li | 2018 | Journal of Landscape Research2018，10，3： | 0 |
| 18 | CRITICAL PROCESSES AND MAJOR FACTORS THAT DRIVE NITROGEN TRANSPORT FROM FARMLAND TO SURFACE WATER BODIES显示文摘Agricultural non-point source pollution is increasingly an important issue affecting surface water quality.Currently,the majority of the studies on nitrogen loss have focused on the agricultural field scale,however,the response of surface water quality at the watershed scale into the nitrogen loss at the field scale is poorly understood.The present study systematically reviewed the critical processes and major factors that nitrogen transport from farm fields to surface water bodies.The critical processes of farmland nitrogen entering surface water bodies involve the processes of nitrogen transport from farmland to ditches and the transformation processes of nitrogen during migration in ditches/rivers.Nitrogen transport from farmland to ditches is one of the prerequisites and critical processes for farmland nitrogen transport to surface water bodies.The transformation of nitrogen forms in ditches/rivers is an intermediate process in the migration of nitrogen from farmland to surface water bodies.Nitrogen loss from farmland is related to soil storage and exogenous inputs.Therefore,nitrogen input management should not only consider the current input,but also the contribution of soil storage due to the historical surpluses.Ditches/rivers have a strong retention capacity for nitrogen,which will significantly affect the process of farmland nitrogen entering surface water bodies.The factors affecting nitrogen transformation in river/ditches can be placed in four categories:(1)factors affecting hydraulic retention time,(2)factors affecting contact area,(3)factors affecting biological activity,and(4)forms and amount of nitrogen loading to river/ditches.Ditch systems are more biologically(including plants and microbes)active than rivers with biological factors having a greater influence on nitrogen transformation.When developing pollution prevention and control strategies,ecological ditches can be constructed to increase biological activity and reduce the amount of surplus nitrogen entering the water body.The present research should be valuable for the evaluation of environment impacts of nitrogen loss and the non-point source pollution control. | Wenchao LI Wen XU Gaofei YIN Xulin ZHANG Zihan ZHANG Bin XI Qiuliang LEI Limei ZHAI Qiang ZHANG Linzhang YANG Hongbin LIU | 2023 | Frontiers of Agricultural Science and Engineering2023，10，4： | 0 |
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