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| 1 | Roles of a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis显示文摘ABA, acting as a stress signal, plays crucial roles in plant resistance to water stress. Because ABA signal production is based on ABA biosynthesis, the regulation of NCED, a key enzyme in the ABA biosynthesis pathway, is normally thought of as the sole factor controlling ABA signal production. Here we demonstrate that ABA catabolism in combination with a synergistic regulation of ABA biosynthesis plays a crucial role in governing ABA signal production. Water stress induced a significant accumulation of ABA, which exhibited different patterns in detached and attached leaves. ABA catabolism followed a temporal trend of exponential decay for both basic and stress ABA, and there was little difference in the catabolic half-lives of basic ABA and stress ABA. Thus, the absolute rate of ABA catabolism, i.e. the amount of ABA catabolized per unit time, increases with increased ABA accumulation. From the dynamic processes of ABA biosynthesis and catabolism, it can be inferred that stress ABA accumulation may be governed by a synergistic regulation of all the steps in the ABA biosynthesis pathway. Moreover, to maintain an elevated level of stress ABA sustained activation of NCED3 should be required. This inference was supported by further findings that the genes encoding major enzymes in the ABA biosynthesis pathway, e.g. NCED3, AAO3 and ABA3 were all activated by water stress, and with ABA accumulation progressing, the expressions of NCED3, AAO3 and ABA3 remained activated. Data on ABA catabolism and gene expression jointly indicate that ABA signal production is controlled by a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism. | REN HuiBo FAN YiJian GAO ZhiHui WEI KaiFa LI GuiFen LIU Jing CHEN Lin LI BingBing HU JianFang JIA WenSuo | 2007 | Chinese Science Bulletin2007，52，4： | 12 |
| 2 | Modulation of Root Signals in Relation to Stomatal Sensitivity to Root-sourced Abscisic Acid in Drought-affected Plants显示文摘到弄干的土壤导致的根信号的有气孔的敏感可以在环境和植物种类之间变化。这是可能的在根信号之中是相互作用和调整的结果。作为一个压力信号, abscisic 酸(骆驼毛的织物) 在根起一个中央作用射击发信号。pH 和水力的信号可以与骆驼毛的织物信号交往并且这样,联合调整有气孔的回答到改变的土壤水地位。pH 本身能被几个因素,在木部的化学组成在之中流修改,包围容器的实时房间起关键作用。除了木部 pH,更多的注意应该对叶 apoplastic pH 的直接调整被给予,因为当没在木部 pH 上有重要效果时,许多化学组成可能强烈修改叶 apoplastic pH。ABA 信号紧张的直接调整可能是更重要的让有气孔的回答的规定玷污比 ABA 信号本身弄干。如果射击弄干的土壤的通讯的一条持续的根在整个植物水平操作,骆驼毛的织物信号被 ABA 分解代谢和先锋的供应也调整到根。更重要地,骆驼毛的织物分解代谢能在 ABA 信号的命运的决心起关键作用并且从而控制采购根的骆驼毛的织物信号的有气孔的行为。 | Huibo Ren Kaifa Wei Wensuo Jia William John Davies Jianhua Zhang | 2007 | Journal of Integrative Plant Biology2007，49，10： | 11 |
| 3 | Localization of NOS-like protein in guard cells of Vicia faba L.and its possible function显示文摘Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of ni- tric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS in- hibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO genera- tion from NOS pathway is the main pathway in the stress and JA responses. The pharmacological ex- periment showed that increasing the Ca2+ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca2+-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses. | LIU Xin WANG YouQun JIA WenSuo LOU ChengHou ZHANG ShuQiu | 2007 | Chinese Science Bulletin2007，52，1： | 5 |
| 4 | CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry显示文摘CRISPR/Cas9-mediated genome editing is a powerful tool for life science research.Recently,strawberry (Fragaria × ananassa),an important horticultural crop,has emerged as a model organism for investigating the regulatory mechanisms of fruit development and ripening (Shulaev et al.,2011;Jia et al.,2013,2017;Kang et al.,2013;Han et al.,2015).While most cultivated strawberries (e.g.,F.× ananassa)are octoploid,most wild strawberries (e.g.,F.vesca)are diploid.Although CRISPR/Cas9 methods have been established in many plants,the use of this tool in strawberry needs to be further explored (Zhou et al.,2018). | Sinian Xing Meiru Jia Lingzhi Wei Wenwen Mao Usman Ali Abbasi Yaoyao Zhao Yating Chen Minglin Cao Kai Zhang Zhengrong Dai Zhechao Dou Wensuo Jia Bingbing Li | 2018 | Journal of Genetics and Genomics2018，45，12： | 4 |
| 5 | Protein phosphorylation is involved in the water stress-induced ABA accumulation in the roots of Malus hupehensis Rehd显示文摘Water stress-induced ABA accumulation plays a key role in the root to shoot communication and/or the cell to cell signaling under the soil stresses. The signaling of the water stress itself that leads to the accumulation, however, is less known. In this study, we subjected the roots of Malus hupehensis seedlings to water stress treatment and investigated the ABA accumulation in relation to protein phos-phorylation. Our results showed that ABA accumulation could be substantially triggered in 40 min and reached 4 folds in 100 min after treatment with 30% PEG 6000 (polyethylene glycol). The water stress treatment also led to a substantial enhancement of total kinase activity, assessed with histone-111 as substrate, in 15 min and a maximum enhancement in 30 min before it declined to initial level. The Ca2+-dependent kinase activity showed a similar, if not more sensitive, trend. When the roots were fed with labeled 32P-ATP, water stress enhanced the labeling of proteins, which showed a maximum labeling at | ZHANG Dapeng, YANG Hongqiang, JIA Wensuo & HUANG Conglin1. Laboratory of Molecular Developmental Biology of Fruit Trees, China Agricultural University, Beijing 100094, China 2. The Key Laboratory of Plant Physiology and Biochemistry of Agricultural Ministry, China Agricultural University, Beijing 100094, China | 2001 | Chinese Science Bulletin2001，46，10： | 4 |
| 6 | Drought tolerance evaluation of tobacco plants transformed with different set of genes under laboratory and field conditions显示文摘尽管广泛的研究证明了许多干旱应答的基因授与干旱忍耐到植物,能力在各种各样的自然条件下面由不同基因授与的干旱忍耐的比较很少有报导。我们评估了并且比较 transgenes,干旱应答的基因(AtDREB1B 和 AtCBL1 ) 和根的二个集合的效果调整建筑学的基因(iaaM 和 AtCKX ) ,在在 Nicotiana tabacum 的干旱忍耐上,植物使遭到了到不同条件。根特定的倡导者 PYK10 驾驶的 AtCKX3 的表示(此后指定了为 P10;P10 : AtCKX3 ) , 35S:AtCKX3,或 P10:iaaM 支持了根生长和开发。比作怀有 P10 的植物: 展出的 AtCKX3, 35S:AtCKX3, P10:iaaM,或空向量,那些带的 35S:AtDREB1B, 35S:AtCBL1,或 35S:iaaM 在控制实验室的条件下面增加了干旱忍耐。相反地,在地里,条件,与 35S:AtDREB1 转变的植物, 35S:AtCBL1,或 35S:iaaM 对干旱应力敏感。在地条件下面,干旱应力戏剧性地减少了生长和而它在带 P10 的植物上有小效果,怀有 35S:AtDREB1B, 35S:AtCBL1, 35S:iaaM,或空向量的植物的种子生产: AtCKX3, 35S:AtCKX3,或 P10:iaaM。这研究证明到有环境条件的干旱压力变化的植物忍耐,和我们的结果显示操作控制根建筑学的基因的表示可能为处于自然条件与改进干旱忍耐设计植物是重要的。 | Yuanhua Wang Ruihong Dang Jinxi Li Yu Han Ning Ding Xingliang Li Meiru Jia Ziqiang Li Lingzhi Wei Jinzhu Jiang Yijuan Fan Bingbing Li Wensuo Jia | 2015 | Science Bulletin2015，60，6： | 3 |
| 7 | Role of ABA in integrating plant responses to drought and salt stresses显示文摘 | Jianhua Zhang Wensuo Jia Jianchang Yang Abdelbagi M. Ismail | 2005 | Field Crops Research2005，，1： | 1 |
| 8 | Role of ABA in integrating plant responses to drought and salt stresses 显示文摘 | Zhang Jianhua Jia Wensuo Yang Jianchang | 2006 | Field Crop Res2006，97，1： | 1 |
| 9 | At MKK1 mediates ABA-induced CAT1 expression and H2O2 production via At MPK6-coupled signaling in Arabidopsis显示文摘 | Xing Yu Jia Wensuo Zhang Jianhua | 2008 | The Plant Journal2008，54，3： | 1 |
| 10 | AtMKK1 and AtMPK6 are involved in abscisic acid and sugar signaling in Arabidopsis seed germination显示文摘 | Yu Xing Wensuo Jia Jianhua Zhang | 2009 | Plant Molecular Biology2009，，6： | 1 |
| 11 | Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits显示文摘Strawberry is increasingly used as a model plant for research on fruit growth and development.The transient gene manipulation(TGM)technique is widely used to determine the function of plant genes,including those in strawberry fruits.However,its reliable application for the precise identification of gene function has been difficult owing to the lack of conditional optimization.In this study,we found that successful transient gene manipulation requires optimization,with the vector type,temperature,and fruit developmental stage being three major factors determining success.Notably,we found that transient gene manipulation was feasible only from the large green fruit stage onwards,making it especially suitable for identifying genes involved in strawberry fruit ripening.Furthermore,we established a method called percentage difference of phenotype(PDP),in which the functional effect of a gene could be precisely and efficiently identified in strawberry fruits.This method can be used to estimate the functional effect of a gene as a value from 0 to 100%,such that different genes can be quantitatively compared for their relative abilities to regulate fruit ripening.This study provides a useful tool for accelerating research on the molecular basis of strawberry fruit ripening. | Yaoyao Zhao Wenwen Mao Yating Chen WeiWang Zhengrong Dai Zhechao Dou Kai Zhang Lingzhi Wei Tianyu Li Baozhen Zeng Ting Liu Yijuan Fan Jiaqi Yan Bingbing Li Wensuo Jia | 2019 | Horticulture Research2019，6，1： | 1 |
| 12 | An effector-reporter system to study cellular signal transduction in strawberry fruit (Fragaria ananassa)显示文摘An effector-reporter system is a powerful tool used to study cellular signal transduction,but this technique has been traditionally used in protoplasts.A similar system to study cellular signal transduction in fruits has not yet been established.In this study,we aimed to establish an effector–reporter system for strawberry fruit,a model nonclimacteric fruit.We first investigated the characteristics of transient gene expression in strawberry fruits and found marked variation in gene expression levels among individual fruits,and this variation has complicated the establishment of a technical system.To overcome this difficulty,we investigated a sampling strategy based on a statistical analysis of the activity pattern of four different reporters(GUS,GFP,FLuc,and RLuc)among individual fruits and combinations of pairs of reporters(GUS/GFP and RLuc/FLuc).Based on an optimized sampling strategy,we finally established a step-by step protocol for the effector/reporter assay.Using FaMYB10 and FaWRKY71 as the effectors and GUS driven by the FaCHS promoter as the reporter,we demonstrated that this effector/reporter system was practical and reliable.This effector/reporter technique will contribute to an in-depth exploration of the signaling mechanism for the regulation of strawberry fruit ripening. | Baozhen Zeng Tianyu Li Wei Wang Zhengrong Dai Jie Li Zhiyuan Xi Kenan Jia Yu Xing Bingbing Li Jiaqi Yan Wensuo Jia | 2021 | Horticulture Research2021，8，1： | 0 |
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