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您的检索式：作者名="Willy Supatto"
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| 1 | Dual-color deep-tissue three-photon microscopy with a multiband infrared laser显示文摘Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology.Threephoton(3P)microscopy with excitation in the short-wavelength infrared(SWIR)water transparency bands at 1.3 and 1.7μm opens up new opportunities for deep-tissue imaging.However,novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach.Here,we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7μm that has characteristics optimized for 3P microscopy:sub-70 fs duration,1.25 MHz repetition rate,and μJ-range pulse energy.In turn,we achieve simultaneous 3P excitation of green fluorescent protein(GFP)and red fluorescent proteins(mRFP,mCherry,tdTomato)along with third-harmonic generation.We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain,chick embryo spinal cord,and live adult zebrafish brain,with an improved signal-to-background ratio compared to multicolor twophoton imaging.This development opens the way towards multiparametric imaging deep within scattering tissues. | Khmaies Guesmi Lamiae Abdeladim Samuel Tozer Pierre Mahou Takuma Kumamoto Karolis Jurkus Philippe Rigaud Karine Loulier Nicolas Dray Patrick Georges Marc Hanna Jean Livet Willy Supatto Emmanuel Beaurepaire Frédéric Druon | 2018 | Light(Science & Applications)2018，7，1： | 3 |
| 2 | Label-free imaging of red blood cells and oxygenation with color third-order sum-frequency generation microscopy显示文摘Mapping red blood cells(RBCs)flow and oxygenation is of key importance for analyzing brain and tissue physiology.Current microscopy methods are limited either in sensitivity or in spatio-temporal resolution.In this work,we introduce a novel approach based on label-free third-order sum-frequency generation(TSFG)and third-harmonic generation(THG)contrasts.First,we propose a novel experimental scheme for color TSFG microscopy,which provides simultaneous measurements at several wavelengths encompassing the Soret absorption band of hemoglobin.We show that there is a strong three-photon(3P)resonance related to the Soret band of hemoglobin in THG and TSFG signals from zebrafish and human RBCs,and that this resonance is sensitive to RBC oxygenation state.We demonstrate that our color TSFG implementation enables specific detection of flowing RBCs in zebrafish embryos and is sensitive to RBC oxygenation dynamics with single-cell resolution and microsecond pixel times.Moreover,it can be implemented on a 3P microscope and provides label-free RBC-specific contrast at depths exceeding 600μm in live adult zebrafish brain.Our results establish a new multiphoton contrast extending the palette of deep-tissue microscopy. | Julia Ferrer Ortas Pierre Mahou Sophie Escoto Chiara Stringari Nicolas BDavid Laure Bally-Cuif Nicolas Dray Michel Negrerie Willy Supatto Emmanuel Beaurepaire | 2023 | Light(Science & Applications)2023，12，2： | 0 |
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