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| 1 | 全国孕产妇死亡监测结果分析显示文摘对1989~1991年全国30省、自治区、直辖市247个监测点1.0亿人口范围内的孕产妇死亡监测资料进行分析。监测地区三年内活产总数4201457例,孕产妇死亡3274例,孕产妇监测死亡率平均为80.0/10万,调整后为87.8/10万。城市、农村、不同地区孕产妇死亡率的高低有明显差异,三年比较,城市、农村孕产妇死亡率呈下降趋势,农村更为明显。孕产妇死亡原因主要为产科出血、好高征、心脏病、产褥感染、羊水栓塞、肝病,产科出血主要原因为胎盘滞留、宫缩乏力。结果显示:可避免的死亡占89.0%,不可避免的死亡占11.0%。并对孕产妇死亡的有关问题进行讨论。 | .Zhang Ling-mei DingHui.(BeiJing Municipal Institute for Women HealthCare , 100006.) | 1994 | 中华妇产科杂志1994，29，9： | 199 |
| 2 | 中国进展期乳腺癌共识指南(CABC 2015)显示文摘在2013年出版的《首届中国进展期乳腺癌共识指南(草案)》(CABC1)的基础上,本指南进一步更新了进展期乳腺癌诊疗过程的一般原则、相关的定义、疗效的评估、不良反应的管理及不同治疗方法的基本策略等内容;专家组系统阅读国内外各种关于进展期乳腺癌的临床研究(包括回顾性的资料分析),整理并总结了各种相关指南,召开会议组织专家进行了多次讨论,将在循证医学基础上达成的专家共识整理成文,为从事乳腺癌专业的医生,尤其是以治疗进展期乳腺癌为主要专业的医生,提供参考。 | China Medical Women′s Association of Clinical Oncology | 2015 | 癌症进展2015，13，3： | 84 |
| 3 | Structure and function of aggrecan显示文摘Aggrecan is the major proteoglycan in the articular cartilage. This molecule is important in the proper functioning of articular cartilage because it provides a hydrated gel structure (via its interaction with hyaluronan and link protein) that endows the cartilage with load-bearing properties. It is also crucial in chondroskeletal morphogenesis during development. Aggrecan is a multimodular molecule expressed by chondrocytes. Its core protein is composed of three globular domains (G1, G2, and G3) and a large extended region (CS) between G2 and G3 for glycosaminoglycan chain attachment. G1 comprises the amino terminus of the core protein. This domain has the same structural motif as link protein. Functionally, the G1 domain interacts with hyaluronan acid and link protein, forming stable ternary complexes in the extracellular matrix. G2 is homologous to the tandem repeats of G1 and of link protein and is involved in product processing. G3 makes up the carboxyl terminus of the core protein. It enhances glycosaminoglycan modification and product secretion. Aggrecan plays an important role in mediating chondrocyte-chondrocyte and chondrocyte-matrix interactions through its ability to bind hyaluronan. | CHRIS KIANI, LIWEN CHEN, YAO JIONG WU, ALBERT J YEE, BURTON B YANG, Sunnybrook and Women’s College Health Sciences Centre and Department of Laboratory Medicine and Patobiology, 2 Department of Surgeny, Faculty of Medicine, University of Toronto, Canada | 2002 | Cell Research2002，12，1： | 27 |
| 4 | Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation显示文摘To evaluate the effects of sperm with different parameters and sources on the outcomes of intracytoplasmic sperm injection (ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522 cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia (OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth, premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring mal-formation will require further study using a larger sample size. | Yue-hong LU1,2, Hui-juan GAO1, Bai-jia LI1, Ying-ming ZHENG1, Ying-hui YE1, Yu-li QIAN1, Chen-ming XU1, He-feng HUANG1, Fan JIN1 (1Department of Reproductive Endocrinology, Key Laboratory of Reproductive Genetics, Ministry of Education, and Key Laboratory of Women’s Reproductive Health of Zhejiang Province, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China) (2Department of Reproductive Health, Shaoxing Women and Children Hospital, Shaoxing 312000, China) | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，1： | 14 |
| 5 | Cloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells显示文摘AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9 ( CYP2 C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2 C9 eDNA was cloned and expressed stably in CHL cellsMETHODS: After extraction of total RNA from human livertissue, the human CYP2C9 eDNA was amplified withreverse transcription-polymerase chain reaction (RT-PCR),and cloned into cloning vector pGEM-T. The cDNA fragmentwas identified by DNA sequencing and subcloned into amammalian expression vector pREP9. A transgenic cell linewas established by transfecting the recombinant vector ofpREP9-CYP2C9 into CHL cells. The enzyme activity ofCYP2C9 catalyzing oxidation of tolbutamide to hydroxytolbutamide in S9 fraction of the cell was determined by highperformance liquid chromatography(HPLC).RESULTS: The amino acid sequence predicted from theeDNA segment was identical to that of CYP2 C9 * 1, the wildtype CYP2 C9. However, there were two base differences, i.e. 21T > C, 1146C > T, but the encoding amino acidsequence was the same, L7, P382. The S9 fraction of theestablished cell line metabolizes tolbutamide to hydroxytolbutamide; tolbutamide hydroxylass activity was found to be0.465 ± 0.109 μmol@ min-1 . g1 S9 protein or 8.62 ± 2.02 mol@ min 1 ~mol-1 CYP, but was undetectable in parental CHL cell.CONCLUSION: The cDNA of human CYP2C9 was successfullycloned and a cell line of CHL- CYP2C9, efficiently expressingthe protein of CYP2C9, was established. | Ge-Jian Zhu Ying-Nian Yu,Department of Pathophysiology and Laboratory of Medical Molecular Biology,Zhejiang University School of Medicine,Hangzhou 310031,Zhejiang Province,China Xin Li,Department of pharmaceutical analysis & drug metabolism,College of Pharmacology Science,Zhejiang University,Hangzhou 310031,Zhejiang Province,China Yu-Li Qian, Present address:Center of laboratory,Women’s hospital,School of Medicine,Zhejiang University,Hangzhou 310031,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 9 |
| 6 | 小额贷款,HIV/AIDS及其家庭的曙光——瑞丽市为HIV/AIDS家庭通过小额贷款促进生产自救模式的探索显示文摘[目的]改善云南省瑞丽地区的HIV/AIDS家庭生活质量低下,严重受吸毒和艾滋病问题的双重困扰。[方法]分别为25个HIV/AIDS家庭和25个普通健康家庭提供了小额无息贷款服务,以发展适合目标家庭需求的创收性生产项目,一年后50个贷款户均如数还清贷款。[结果]在项目实施中充分体现了社区居民的参与和互助,开展多种形式的人文关怀活动和艾滋病预防活动。[结论]小额贷款对在边疆少数民族地区开展艾滋关怀项目进行了有益地探索。 | Yunnan Center of Women & Children Development,(Yunnan Center of Women & Children Development,Kunming 650032,China) | 2006 | 卫生软科学2006，20，1： | 8 |
| 7 | Clinical Observation of Pregnancy with Chlamydia Trachomatis Infection.显示文摘From 1992 to 1993, 1 389 cases of pregnancy at 12-20 gestational weeks were investigated. At first antenatal visit the samples of cervical secretion were taken for chlamydia trachomatis antigen test and blood samples were tested for antidody simultaneously. Micro immunofluorescence | Ding Ying et al. Shanghai Changning District Women’s Hospital, Shanghai 200052 | 1995 | Chinese Medical Journal1995，，7： | 6 |
| 8 | Functional distinction of rat liver natural killer cells from spleen natural killer cells under normal and acidic conditions in vitro显示文摘BACKGROUND: The microenvironment within solid tumors has often been shown to exhibit an acidic extracellular pH. Although the morphologic and functional differences in natural killer (NK) cells of the liver and spleen have been reported previously under physiological conditions, the difference under acidic conditions is still unclear. This study was to investigate the differences in the morphological and functional characteristics between rat liver and spleen NK cells under normal and acidic conditions in vitro. METHODS: Liver and spleen NK cells were isolated and purified from Sprague-Dawley rats by density gradient centrifugation and the Dynabeads FlowComp TM Flexi system, and stimulated for 4 days with or without IL-2 or treated with low pH or control for different times. Morphology was examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cell death and proliferation assays were performed by flow cytometry, IFN-γ production was tested by ELISA, and cytotoxic activity was evaluated by lactate dehydrogenase (LDH) release assay. RESULTS: Liver NK cells had significantly higher levels of cytotoxic activity than spleen NK cells under normal and acidic conditions, and the maximum difference was observed at pH 5.6. Further analysis revealed that the cytotoxic activity of NK cells was correlated with morphology, cell death, proliferative activity and IFN-γ production. By TEM, liver NK cells contained a greater number of electron-dense granules per cell at pH 5.6.Moreover, a modest elevation of cell death and reduction of proliferation of liver NK cells occurred within a range of 5.6-7.2. Interestingly, an acidic extracellular pH only marginally, and not significantly, suppressed IFN-γ production by liver NK cells. CONCLUSION: The sharp morphological and functional differences shown by the two types of NK cells in vitro indicate that liver NK cells are unexpectedly resistant to pH shock. | Li-Hong Lv, Jian-Dong Yu, Guo-Lin Li, Tian-Zhu Long, Wei Zhang, Ya-Jin Chen, Jun Min and Yun-Le Wan Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Department of Breast Surgery, Guangzhou Women and Children’s Medical Center Guangzhou 510120, China Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 5 |
| 9 | Nonlinear dynamics of self-oscillating polymer gels显示文摘Self-oscillating polymer gels driven by Belousov-Zhabotinsky (BZ) chemical reaction are a new class of functional gels that have a wide range of potential applications (e.g., autonomously functioning membranes, actuate artificial muscles). However, the precise control of these gels has been an issue due to limited investigations of the influences of key system parameters on the characteristics of BZ gels. To address this deficiency, we studied the self-oscillating behavior of BZ gels using the nonline-ar dynamics theory and an Oregonator-like model, with focus placed upon the influences of various system parameters. The analysis of the oscillation phase indicated that the dynamic response of BZ gels represents the classical limit cycle oscillation. We then investigated the characteristics of the limit cycle oscillation and quantified the influences of key parameters (i.e., ini-tial reactant concentration, oxidation and reduction rate of catalyst, and response coefficient) on the self-oscillating behavior of BZ gels. The results demonstrated that sustained limit cycle oscillation of BZ gels can be achieved only when these key pa-rameters meet certain requirements, and that the pattern, period and amplitude of the oscillation are significantly influenced by these parameters. The results obtained in this study could enable the controlled self-oscillation of BZ gels system. This has several potential applications such as controlled drug delivery, miniature peristaltic pumps and microactuators. | WANG PengFei1, ZHOU JinXiong1, LI MeiE2, XU Feng1,3 & LU TianJian1 1 Biomedical Engineering and Biomechanics Center, SV Laboratory, Xi’an Jiaotong University, Xi’an 710049, China 2 School of Materials Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, China 3 HST Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, 510660, USA | 2010 | Science China(Technological Sciences)2010，53，7： | 3 |
| 10 | Donor liver natural killer cells alleviate liver allograft acute rejection in rats显示文摘BACKGROUND:Liver enriched natural killer (NK) cells are of high immune activity.However,the function of donor liver NK cells in allogeneic liver transplantation (LTx) remains unclear.METHODS:Ten Gy of whole body gamma-irradiation (WBI) from a 60 Co source at 0.6 Gy/min was used for depleting donorderived leukocytes,and transfusion of purified liver NK cells isolated from the same type rat as donor (donor type liver NK cells,dtl NKs) through portal vein was performed immediately after grafting the irradiated liver.Post-transplant survival observation on recipients and histopathological detection of liver grafts were adoptive to evaluate the biological impact of donor liver NK cells on recipients' survival in rat LTx.RESULTS:Transfusion of dtl NKs did not shorten the survival time among the recipients of spontaneous tolerance model (BN to LEW rat) after rat LTx,but prolonged the liver graft survival among the recipients depleted of donor-derived leukocytes in the acute rejection model (LEW to BN rat).Compared to the recipients in the groups which received the graft depleted of donor-derived leukocytes,better survival and less damage in the allografts were also found among the recipients in the two different strain combinations of liver allograft due to transfusion of dtl NKs.CONCLUSIONS:Donor liver NK cells alone do not exacerbate liver allograft acute rejection.Conversely,they can alleviate it,and improve the recipients' survival. | Jian-Dong Yu,Tian-Zhu Long,Guo-Lin Li,Li-Hong Lv,Hao-Ming Lin,Yong-Heng Huang,Ya-Jin Chen and Yun-Le Wan Department of Hepatobiliary Surgery,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China Department of Breast Surgery,Guangzhou Women and Children’s Medical Center,Guangzhou 510120,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，4： | 2 |
| 11 | Risk and benefits of estrogen plus progestin in healthy postmenopausal women principal results form the women' s health initiative randomized cotrolled trial显示文摘 | | 2002 | JAMA2002，288，3： | 1 |
| 12 | PROOF study Group 显示文摘 | Chesnut CH Silveman S Andviano k A vandomized tial of nasal spay salmon calcitonin in postmenopausal women with established osteporosis: the prevnt recurrence of osteoporasis farcture study | 2000 | Am J Med2000，109，6： | 1 |
| 13 | Risks and benefits of estrogen plus progesterone in healthy post-menopausal women 显示文摘 | Writing Group for the Women's Health Initiative Investigators | 2002 | JAMA2002，288，3： | 1 |
| 14 | Dietary fiber and risk of breast cancer in the UK Women's Cohort Study 显示文摘 | Cade JE Burley VJ Darren Charles Greenwood the UK Women's Cohort Study Steering Group | 2007 | International Journal of Epi- demiology2007，36，： | 1 |
| 15 | Am J显示文摘 | Galdwell SH Hespenheide EE Subacute liver failure in obese women | 2002 | Gastroenterol2002，97，： | 1 |
| 16 | ECPPA (Estudo Colaborativo pare Preveneao da Preeclampsia corn Aspirina) Collaborative Group 显示文摘 | ECPPA : randomised trial of low dose aspirin for the prevention of maternal and fetal complications in high risk pregnant women | 1996 | Br J Obstet Gynaecol1996，103，1： | 1 |
| 17 | Risks and benefits of estrogen plus progestin in healthy postmenopausal woman: prineiple results from the Woman's Health Initiative randomized eontrolled trial显示文摘 | The Writing Group for the Women's Health Initiative Investigators | 2002 | JAMA2002，288，： | 1 |
| 18 | Aspirintherapy does less than anticipated in preventing heart attacks inwomen-but more than we knew in warding off strokes显示文摘 | Aspirin study refocuses prevention message for women | 2005 | HarvWomens Health Watch2005，12，9： | 1 |
| 19 | Effects of conjugated equine estrogen in postmenopausal women with hysterectomy: the Women's Health Initiative randomized controlled trial 显示文摘 | Anderson GL Limacher M Assaf AR Women' s Health Initiative Steering Committee | 2004 | JAMA2004，291，14： | 1 |
| 20 | Risks and benefits of estrogen plus progestin in healthy postmenopausal women-Principal results from the Women's Health Initiative randomized controlled trial 显示文摘 | Writing Group for the Women 's Health Initiative Investigators | 2002 | JAMA2002，288，： | 1 |
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