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| 1 | Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma显示文摘AIM To observe the growth suppression effect of exogenous introduction of early growth response gene-1 (Egr-1 gene) on esophageal carcinoma tissue as well as on esophageal carcinoma cell line Eca109 and to explore the potential application of Egr-1 gene in gene therapy of tumor.METHODS Eukaryotic expression vector of PCMV-Egr-1 plasmid was introduced into Eca109 cell line which expressed no Egr-1 protein originally with lipofectamine transfection method. The introduction and expression of PCMV-Egr-1 plasmid into Eca109 cell line was confirmed by G418 selection culture, PCR amplification of neogene contained in the vector, Western blot analysis and immunocytochemical analysis. The cell growth curve,soft agar colony formation rate and tumorigenicity in SClD mice were examined to demonstrate the growth suppression effect of exogenous Egr-1 gene on Eca109 cell line. The Egr-1 mRNA and Egr-1 protein were also detected in 50 surgical specimens of esophageal carcinoma by in situ hybridization and immunohistochemistry.RESULTS Exogenous Egr-1 gene was introduced successfully into Eca109 cell line and expressed Egr-1 protein stably. The transfected Eca109 cell line grew more slowly than control Eca109 as shown by cell growth curves, the soft agar colony formation rate (4.0% vs 6.9%, P<0.01) and the average growth rate of tumor in SCID mice (35.5 ± 7.6 vs 65.8 ± 7.6, P<0.05). The expression level of Egr-1 mRNA and protein significantly increased in dysplastic epithelia adjacent to cancer rather than in cancer tissues (65.8% vs 20.0% by ISH and 57.9% vs 14.0% by IHC, P<0.01).CONCLUSION Exogenous Egr-1 gene shows the strong effect of growth inhibition in Eca109 cell line. Egr-1 in the cancer tissue shows down-regulated expression that supports the inhibited function of Egr-1 in cancer growth and suggests Egr-1 may have an important role in gene therapy of esophageal carcinoma. | Ming-Yao Wu Mao-Huai Chen Ying-Rui Liang Guo-Zhao Meng Huan-Xing Yang Chu-Xiang Zhuang Department of Pathology,Shantou University Medical College,Shantou 515031,Guangdong Province,China Supported by the National Natural Science Foundation of China,No.39670298. | 2001 | World Journal of Gastroenterology2001，7，4： | 20 |
| 2 | Hepatocellular carcinoma HepG2 cell apoptosis and caspase-8 and Bcl-2 expression induced by injectable seed extract of Coix lacryma-jobi显示文摘BACKGROUND:Many Chinese herbs,especially herbal injections,have been shown to have anti-tumor effects in recent years.However,since most reports focus on the clinical effectiveness of these herbs,their mechanisms of action are not well understood.In this study,we assessed apoptosis in the hepatocellular carcinoma (HCC) cell line HepG2 induced by an injectable extract from the seed of Coix lacryma-jobi (Semen coicis,SC),and monitored the expression of Bcl-2 and caspase-8.METHODS:Injectable SC was applied to HepG2 cells at different concentrations and the cells were collected 12,24 and 48 hours later.5-fluorouracil was used as a positive control group,and fluorescence-activated cell-sorting cytometry was used to measure the apoptosis rate of HepG2 cells and the expression of Bcl-2 and caspase-8 proteins.RESULTS:SC induced apoptosis in HepG2 cells in a concentration and time-dependent manner,and the expression of caspase-8 was elevated and prolonged.However,it did not significantly influence the expression of Bcl-2.CONCLUSION:Injectable SC may induce apoptosis in HCC cells by regulating the expression of caspase-8. | Department of General Surgery (Lu Y and Zhang BY),Department of Bio-Information (Jia ZX),Affiliated Medical College Hospital,Qingdao University,Qingdao 266003,China Shanghai Jiaotong University School of Medicine,Shanghai 200025,China (Wu WJ) Department of Medicine,Heze Medical College,Heze 274000,China (Lu ZQ) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 17 |
| 3 | Variations of δ^(18)O in precipitation from the Muztagata Glacier, East Pamirs显示文摘Expeditions to Muztagata (in the eastern Pamirs) during the summer seasons of 2002 and 2003 collected precipitation samples and measured their oxygen isotopes. The δ 18O in precipita-tion displays a wide range, varying from ?17.40‰ to +1.33‰ in June-September 2002 and from ?22.31‰ to +4.59‰ in May-August 2003. The δ 18O in precipitation correlates with the initial tem-perature of precipitation during the observing periods. The positive correlation between δ 18O and tem- perature suggests that δ 18O can be used as an indicator of temperature in this region. The δ 18O values in fresh-snow samples collected from two snow events at different elevations on the Muztagata Gla-cier show a strong “altitude effect”, with a ratio of nearly ?0.40‰ per 100 m from 5500 m to 7450 m. | LI Zhen1, YAO Tandong1,2, TIAN Lide1,2, XU Baiqing1,2 & Wu Guangjian2 1. Key Laboratory of Cryosphere and Environment, Jointly Established by Cold and Arid Regions Environmental and Engi-neering Research Institute and Institute of Tibetan Plateau Research, Chinese Academy of Sciences and Chinese Academy of Meteorological Sciences, China Meteorological Administration, CAS, Lanzhou 730000, China 2. Laboratory of Environment and Process on Tibetan Plateau, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100085, China | 2006 | Science China Earth Sciences2006，49，1： | 14 |
| 4 | ENSO events and interannual variations of winter sea-ice in the Greenland, the Kara and the Barents Seas显示文摘A number of researches show that ENSO is a phenomenon related not only to interannual variations in the tropical atmosphere and ocean, but also to climatic anomalies in the high latitudes. On the one hand, ENSO results in a teleconnection in the middle-high latitudes; on the other hand, the atmospheric circulation anomalies in the middle-high latitudes play an impor- | Wu, BY Gao, DY Huang, RH | 1997 | Chinese Science Bulletin1997，42，16： | 9 |
| 5 | Intrahepatic transplantation of hepatic oval cells for fulminant hepatic failure in rats显示文摘AIM:To evaluate the effect of intrahepatic transplantation of hepatic oval cells(HOC)on fulminant hepatic failure(FHF)in rats.METHODS:HOC obtained from rats were labeled with green fluocescent protein(GFP)or 5,6carboxyfluorescein diacetate succinmidyl ester(CFDASE).Cell fluorescence was observed under fluorescent microscope at 6,24,48 and 72 h after labeling.CFDASE labeled HOC(5×10 6 cells each rat)were injected into livers of rats with FHF induced by D-galactosamine.Serum albumin(ALB),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBil)levels were measured at different time points.Liver function of rats was examined on days 3,7,14 and 21 after HOC transplantation.RESULTS:The positive rate of GFP and CFDA-SE labeled HOC was 10%and 90%,respectively,with no significant change in cell viabilities.The survival rate was higher in HOC transplantation group than in control group,especially 48(9/15 vs 6/15)and 72 h(9/15 vs 4/15)after HOC transplantation.The serum ALT,AST and TBil levels were decreased while the serum Alb level was increased after HOC transplantation.Fluorescence became faded and diffused in liver tissues,suggesting that proliferation and differentiation occur in transplanted HOC.CONCLUSION:CFDA-SE is superior to GFP in labeling HOC,although fluorescence intensity is decreased progressively with cell division.HOC transplantation can improve the liver function and increase the survival rate of recipients. | Chen-Xuan Wu,Qi Zou,Zheng-Yan Zhu,Ying-Tang Gao,Yi-Jun Wang,Department of Hepatobiliary,The Third Center of Affiliated Hospital of Tianjin Medical University Key Laboratory of Artificial Cells of Tianjin,Tianjin 300170,China Author contributions:Wang YJ,Wu CH and Zou Q designed the research Zou Q,Zhu ZY,Gao YT performed the research Zou Q analyzed the data Wang YJ and Wu CX wrote the paper.Supported by Tianjin Science Committee,Grant No.05SYSYJC02600 and Tianjin Health Bureau,Grant No.05KYR01 | 2009 | World Journal of Gastroenterology2009，15，12： | 7 |
| 6 | FINITENESS CONDITIONS FOR GENERALIZED EXPONENTS OF DIGRAPHS显示文摘Necessary and sufficient conditions are given for the finiteness of the generalized exponents expD(k), f(D, k) and F(D, k) for digraphs which are not necessarily primitive. Also the largest finite value of the generalized exponent expD(k) for digraphs of order n is determined and the complete characterizations of the extreme digraphs are given. | SHAO JIAYU (Department of Applied Mathematics, Tonsil University, Shanghai 200092, China.)WU XIAOJUN (Project supported by the National Natural Science FOundation of China and Shanghai Science anda’echnolgy Developing FOundation.) | 1998 | Chinese Annals of Mathematics,Series B1998，19，4： | 5 |
| 7 | Loose architecture of multi-level massive geospatial data based on virtual quadtree显示文摘This paper proposed a virtual quadtree (VQT) based loose architecture of multi-level massive geospatial data for integrating massive geospatial data dispersed in the departments of different hierarchies in the same sector into a unified GIS (Geographic Information System) platform. By virtualizing the nodes of the quad-tree,the VQT separates the structure of data organization from data storage,and screens the difference between the data storage in local computer and in the re-mote computers in network environment. And by mounting,VQT easily integrates the data from the remote computers into the local VQT so as to implement seam-less integration of distributed multi-level massive geospatial data. Based on that mode,the paper built an application system with geospatial data over 1200 GB distributed in 12 servers deployed in 12 cities. The experiment showed that all data can be seamlessly rapidly traveled and performed zooming in and zooming out smoothly. | YANG ChongJun,WU Sheng,REN YingChao,FU Li,ZHANG FuQing,WANG Gang,TAN Jian,LIU DongLin,MA ChaoJi & LIANG Li State Key Laboratory of Remote Sensing Science,Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China | 2008 | Science China(Technological Sciences)2008，51，S1： | 4 |
| 8 | Grain size record of microparticles in the Muztagata ice core显示文摘The dust transport and sediment characteristics are discussed based on analysis of mi-croparticle size and size distribution in the Muztagata ice core at 6350 m a.s.l. The finer particles with diameter of 1―5 μm are the dominant fraction in number, while middle and coarse particles mainly contribute to the total volume. The lognormal distribution characteristics can be seen for some high concentration samples, showing that model size and standard variation are greater than that in the Greenland ice cores. However, size-volume distribution of some low concentration samples is ab-normal. Those distributions reflect the dust deposit process in high mountain glaciers at mid-low lati-tudes and show differences from those in polar ice sheet. | WU Guangjian1,2, YAO Tandong1,2, XU Baiqin1,2, LI Zheng2, TIAN Lide1,2, DUAN Keqin2 & WEN Linke2 1. Laboratory of Environment and Process on Tibetan Plateau, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100085, China 2. Key Laboratory of Cryosphere and Environment, Jointly Established by Cold and Arid Regions Environmental and Engi-neering Research Institute and Institute of Tibetan Plateau Research, Chinese Academy of Sciences and Chinese Academy of Meteorological Sciences, China Meteorological Administration, Lanzhou 730000, China | 2006 | Science China Earth Sciences2006，49，1： | 3 |
| 9 | Preparation of MWNTs/Al_2O_3 composites and their mechanical and electrical properties显示文摘The mechanical and electrical properties of MWNTs/Al2O3 composite prepared fabricated by hot pressing are investigated. The relation between properties and microstructure of composites is also discussed. With 4wt% MWNTs as reinforcement, the fracture toughness of composite obtained reaches 5.55 MPa·m1/2, which is 80% higher than that of pure Al2O3 obtained in the same conditions. The main toughening mechanism is CNTs’ pinning alumina grain boundaries, and the pullout of CNTs takes effect also. Through adding 2wt% MWNTs and altering the mix method, the fracture toughness of the composite obtained is 3.97 MPa·m1/2. Furthermore, the electrical resistivity is as low as 8.4×10?3 ?·m, decreasing by 14 orders of magnitude compared with pure alumina ceramics. The function of CNTs in composite is related to the distribution state of CNTs in composite, and the distribution state of CNTs in matrix is dependent on preparation procedures. | FAN Jinpeng1,2, ZHAO Daqing1,2, XU Zening1,2 & WU Minsheng1,2 1. Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China 2. Key Laboratory for Advanced Manufacturing by Materials Processing Technology, Tsinghua University, Beijing 100084, China | 2005 | Science China(Technological Sciences)2005，48，6： | 2 |
| 10 | 北美流行的散热器及供暖设计方案显示文摘介绍了美国、加拿大流行的供暖设计方案及其所用的散热器,对比了国内现行的散热器及在高层建筑中常用的垂直串联单管系统,并提出了供国内设计界参考的意见。 | By Wu Zhanhao and Zhu Bingfan | 1998 | 暖通空调1998，28，4： | 2 |
| 11 | THE NUMERICAL METHODS FOR SOLVING EULER SYSTEM OF EQUATIONS IN REPRODUCING KERNEL SPACE H^2(R)显示文摘A new method is presented by means of the theory of reproducing kernel space and finite difference method, to calculate Euler system of equations in this paper. The results show that the method has many advantages, such as higher precision, better stability, less amount of calculation than any other methods and the reproducing kernel function has good local properties and its derived function is wavelet function. | Wu, BY Zhang, QL | 2001 | Journal of Computational Mathematics2001，19，3： | 2 |
| 12 | In?uence of maternal antibody against hepatitis B surface antigen on active immune response to hepatitis B vaccine in infants 显示文摘 | Hu YL Wu QZ Xu BY | 2008 | Vaccine2008，26，48： | 1 |
| 13 | Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongation factor 1 alpha l显示文摘 | Lin WS Jiao BY Wu YL | 2012 | J Med Virol2012，84，6： | 1 |
| 14 | DNA methylation of PAX1 as a biomarker for oral squamous cell carcinoma显示文摘 | Huang YK Peng BY Wu CY | | 0，，08： | 1 |
| 15 | Predictive value of rapid shallow breathing index measured at initiation and termination of a 2-hour spontaneous breathing trial for weaning outcome in ICU patients显示文摘 | Kuo PH Wu HD Lu BY | | 0，，： | 1 |
| 16 | Increased matriptase zymogen activation in inflammatory skin disorders 显示文摘 | Chen CJ Wu BY Tsao PI | 2011 | Am J Physiol Cell Physiol2011，300，3： | 1 |
| 17 | Quercetin inhibits c-fos,heat shock protein,and glial fibrillary acidic protein expression in injured astrocytes显示文摘 | Wu BY Yu AC | 2000 | J Neurosci Res2000，62，5： | 1 |
| 18 | Multi-lipofection efficiently transfected genes into astrocytes in primary culture显示文摘 | Wu BY Liu RY So KL | 2000 | J Neurosci Methods2000，102，2： | 1 |
| 19 | Quantitative analysis of immunocompetent cells in oral submucous fibrosis in Taiwan显示文摘 | Chiang CP Wu HY Liu By | 2002 | Oral Oncol2002，38，1： | 1 |
| 20 | B-type natriuretic peptide and its clinical utility in patients with heart failure显示文摘 | Alan BY Wu HB PhD | 2001 | CE Cardiac Markers2001，10，1： | 1 |
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