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| 1 | Metabolic profiling analysis of a D-galactosamine/lipopolysaccharide- induced mouse model of fulminant hepatic failure显示文摘 | Feng, B Wu, SM Lv, S Liu, F Chen, HS Yan, XZ Li, Y Dong, FT Wei, L | 2007 | 中国生物学文摘2007，21，11： | 23 |
| 2 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 3 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 4 | A systems biology perspec- tive on sVEGFR1 : its biological function, pathogenic role and ther- apeutic use显示文摘 | Wu FT Stefanini MO Mac F | 2010 | Cell Mol Med2010，14，3： | 1 |
| 5 | Gastroenteritis in a Taipei emergency department: aetiology and risk factors 显示文摘 | Lai CC Wu FT Ji DD | 2011 | Clin Microbiol Infect2011，17，： | 1 |
| 6 | Hypercholesterolemia accelerates vascular calcification induced by excessive vitamin D via oxidative stress显示文摘 | Tang FT Chen SR Wu XQ | | 0，，05： | 1 |
| 7 | A systems biology perspective on sVEGFR1:its biological function,pathogenic role and therapeutic use显示文摘 | Wu FT Stefanini MO Mac GF | 2010 | Cell Mol Med2010，14，3： | 1 |
| 8 | Prognostic factors of heart failure with preserved ejection fraction:a 12-year pro- spective cohort follow-up study显示文摘 | WU CK LEE JK CHIANG FT | 2014 | Int J Cardiol2014，17，3： | 1 |
| 9 | Tanshinone II A attenuates atherosclerotic calcification in rat model by inhibition of oxidative stress 显示文摘 | Tang FT Wu XQ Wang TQ | 2007 | Vasc Pharrnacol2007，46，： | 1 |
| 10 | Comparison between serotyping method and multiplex Real Time PCR to identify diarrheagenic gscherichia coli in Taiwan显示文摘 | Yang JR Wu FT Tsai JL | 2007 | J Clin Microbiol2007，，45： | 1 |
| 11 | Gastroenteritis in a Taipei emergency department: aetiology and risk factors 显示文摘 | Lai CC Wu FT Ji DD | 2011 | Clin Microbiol Infect2011，17，7： | 1 |
| 12 | The Radioact-ive Labeling of Proteins With an Iodinated Amidination Reagent显示文摘 | Wood FT Wu MM Gerhart JC | 1975 | Anal Biochem1975，69，： | 1 |
| 13 | ITF-2,a downstream target of the Wnt/TCF pathway,is activated in human cancers with beta-catenin defects and promotes neoplastic transformation显示文摘 | Kolligs FT Nieman MT Winer I Hu G Van Mater D Feng Y Smith IM Wu R Zhai Y Cho KR Fearon ER | 2002 | Cancer Cell2002，1，： | 1 |
| 14 | Tanshinone Ⅱ A attenuates atherosclerotic calcification in rat model by inhibition of oxidative stress显示文摘 | Tang FT Wu XQ Wang TQ | 2007 | Vascular Pharmacology2007，4，6： | 1 |
| 15 | A systems biology perspective on SVEGFRI : its biological function , function , pathogenic role and therapeutic use显示文摘 | Wu FT Stefanini MO Mac Gabhann F | 2010 | J Cell Mol Med2010，14，3： | 1 |
| 16 | Effect of blocking medial area of nucleus retrofacialis on respiratory rhythm 显示文摘 | Zhang FT Wu ZH | 1991 | Res Physiol1991，85，1： | 1 |
| 17 | Hypercholesterolemia accelerates vascular calcification induced by excessive vitamin D via oxidative stress 显示文摘 | Tang FT Chen SR Wu XQ | 2006 | Calcif Tissue Int2006，79，5： | 1 |
| 18 | The effect of elevated extracellular glucose on adherens junction proteins in cultured ratheart endothelial cells 显示文摘 | Lee HZ Yeh FT Wu CH | 2004 | Life Sci2004，74，17： | 1 |
| 19 | A systems biology perspective on sVEGFR1 : Its biological function, pathogenic role and therapeutic use 显示文摘 | Wu FT Stefanini MO Mac Gabhann F | 2010 | J Cell Mol Med2010，14，3： | 1 |
| 20 | Plasma levels of tumor necrosis factor-a and interleukin - 6 are associated with diastolic heart failure through downregulation of sarcoplasmic reticulum CaH ATPase显示文摘 | Wu CK LeeJK Chiang FT | 2011 | Crit Care Med2011，39，5： | 1 |
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