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| 1 | A metabonomic investigation on the biochemical perturbation in liver failure patients caused by hepatitis b virus显示文摘 | Yu, K Sheng, GP Sheng, JF Chen, YM Xu, W Liu, XL Cao, HC Qu, HB Cheng, YY Li, LJ | 2007 | 中国生物学文摘2007，21，11： | 25 |
| 2 | Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression显示文摘AIM To investigate the mechanisms ofsalvianolic acid A(SA-A)against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10-4mol/L-10-7mol/L SA-A for 22 h.The cell viability wasassayed by[3H]proline incorporation,cellproliferation by[3H]TdR incorporation,cellcollagen synthetic rate was measured with[3H]proline impulse and collagenase digestionmethod.The total RNA was prepared from thecontrol cells and the drug treated cellsrespectively,and α(1)I pro-collagen mRNAexpression was semi-quantitatively analyzedwith RT-PCR.RESULTS 10-4mol/L SA-A decreased cellviability and exerted some cytotoxiciy,while10-5mol/L-10-7mol/L SA-A did not affect cellviability,but inhibited cell proliferationsignificantly,and 10-6mol/L SA-A had the besteffect on cell viability among theseconcentrations of drugs.10-5mol/L-10-6mol/LSA-A inhibited intracellular collagen syntheticrate,but no significant influence on extracellularcollagen secretion.Both 10-5mol/L and10-6mol/L SA-A could decrease α(1)I pro-collagen mRNA expression remarkably.CONCLUSION SA-A had potent action againstliver fibrosis.It inhibited NIH/3T3 fibroblastproliferation,intracellular collagen syntheticrate and type I pro-collagen gene expression,which may be one of the main mechanisms of thedrug. | Liu CH Hu YY Wang XL Liu P Xu LM | 2000 | World Journal of Gastroenterology2000，6，3： | 25 |
| 3 | Association of N-terminal pro-brain natriuretic peptide with the severity of coronary artery disease in patients with normal left ventricular ejection fraction显示文摘 | Wu NQ Guo YL Li XL Liu J Qing P Xu RX Zhu CG Jia Y J Liu G Dong Q Jiang LX Li J J Ma FL | 2014 | Chinese Medical Journal2014，，4： | 23 |
| 4 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 5 | Isolation and Structure Elucidation of Autolytimycin, A New Compound Produced by Streptomyces Autolyticus JX-47显示文摘Autolytimycin 1 was isolated from the culture filtrate of Streptomyces autolyticus JX-47, together with two known compounds, lebstatin 2 and 17-O-demethyl-geldanamycin 3. These compounds showed the activities of anti-HSV-I. The structure of 1 was determined by spectral analysis. | Li, MG Wu, SH Zhao, LX Zhang, Q Li, WJ Cui, XL Xu, LH Wu, DG Jiang, CL | 2001 | Chinese Chemical Letters2001，12，10： | 10 |
| 6 | Repairing of goat tibial bone defects with BMP-2 gene-modified tissue-engineered bone显示文摘 | Dai KR Xu XL Tang TT Zhu ZA Yu CF Lou JR Zhang XL | 2006 | 中国生物学文摘2006，20，5： | 2 |
| 7 | Effects of 2,3,4',5-tetrahydroxystilbene 2-O-beta-D-glueoside on vascular endothelial dysfunction in atherogenic-diet rats显示文摘 | Zhang W Xu XL Wang YQ | 2009 | Epub2009，75，11： | 1 |
| 8 | Pharmacokinetics and bioavailability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats 显示文摘 | Xu QF Fang XL Chen DF | 2003 | J Ethnopharmacol2003，84，23： | 1 |
| 9 | Effects of nitrogen fertiliser and wheat straw application on CH4 and N20 emissions from a paddy rice field显示文摘 | Ma J Li XL Xu H Han Y Cai ZC Yagi K | 2007 | Australian Journal of Soil Research2007，45，： | 1 |
| 10 | Correlation between anti- tumor activity, molecular weight, and conformation of lenti- nan 显示文摘 | Zhang LN Li XL Xu XJ | 2005 | Carbohyd Res2005，340，8： | 1 |
| 11 | Expression of type IV collagen, metalloproteinase-2, metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in laryngeal squamous cell carcinomas显示文摘 | CAO XL XU RJ ZHENG vv | 2011 | Asian PacJ Cancer Prev2011，12，2： | 1 |
| 12 | Iatrogenic mandibular fracture associated with third molar removal after mandibular angle osteotectomy显示文摘 | Xu J J Teng L Jin XL | 2014 | The Journal of craniofacial surgery2014，25，3： | 1 |
| 13 | Phenolic antioxidants from the whole plant of Phyllanthus urinaria显示文摘 | Xu M Zha Z J Qin XL | 2007 | Chem Biodiver2007，4，9： | 1 |
| 14 | Epigenetic regulation of the endothelial nitric oxide synthase gene in persistent pulmonary hypertension of the newborn rat显示文摘 | Xu XF Ma XL Shen Z | | 0，，11： | 1 |
| 15 | Activation of NF-kappaB and respirato- ry burst following AspergiUus fumigatus stimulation of macrophages 显示文摘 | Sun H Xu XY Tian XL et ai | 2014 | Immunobiology2014，219，1： | 1 |
| 16 | Pharmacokinetics and bioavailability of genocide Rbl and Rgl from Pan ax notoginseng in rats显示文摘 | Xu QF Fang XL Chen DF | 2003 | J Ethpharm2003，84，： | 1 |
| 17 | In vivo biodistribution and synergistic toxicity of silica nanoparticles and cadmium chloride inmice显示文摘 | Guo MC Xu XL Yan XC | 2013 | J Hazard Mater2013，260，： | 1 |
| 18 | Factors influencing rising caesarean section rates in China between 1988 and 2008显示文摘 | Feng XL Xu L Guo Y | 2012 | Bull World Health Organ2012，90，1： | 1 |
| 19 | Soil organic carbon active fractions as early indicators for total carbon change under straw incorporation显示文摘 | Xu MG Lou YL Sun XL Wang W Baniyamuddin M Zhao K | 2011 | Biology and Fertility of Soils2011，47，7： | 1 |
| 20 | A Systematic review and nalysis of unilateral versus bilateral pedicle screw fixation in transforaminal lumbar interbody fusion 显示文摘 | Hu XQ Wu XL Xu C | 2014 | PloS One2014，9，87： | 1 |
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