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| 1 | Arabidopsis Extra Large G-Protein 2 (XLG2) Interacts with the Gβ Subunit of Heterotrimeric G Protein and Functions in Disease Resistance显示文摘Heterotrimeric GTP 有约束力的蛋白质,由 G, G,和 G 子单元组成,在细胞外的信号由房间表面受体察觉了进细胞内部的生理的回答的 transducing 起重要作用。除了单个原型的 G 蛋白质(GPA1 ) , Arabidopsis 有三唯一的象 G 一样蛋白质,作为 XLG1, XLG2,和 XLG3 知道,那被发现了在原子核局部性,尽管他们行动的功能和模式仍然保持大部分未知。通过 transcriptomic 分析,我们发现 XLG2 和 XLG3 被感染很快与细菌的病原体 Pseudomonas syringae 导致,而 XLG1 抄本水平没被病原体感染影响。一幅反向的基因屏幕表明 xlg2 loss-of-function 变化原因提高了危险性到 P。syringae。介绍的 Transcriptome 表明 xlg2 变化影响防卫相关的基因的一个小集合的被触发病原体的正式就职。然而, xlg1 和 xlg3 异种没在抵抗从野类型的植物显示出差别到 P。syringae。另外, xlg2 xlg3 两倍异种和 xlg1 xlg2 xlg3 三元组异种不与在疾病抵抗显型的 xlg2 单身者异种显著地不同,建议如果有的话,在防卫的 XLG1 和 XLG3 的角色不比为 XLG2 重要。XLG2 的组成的 overexpression 从多重防卫相关的基因导致反常抄本的累积。通过 co-immunoprecipitation 试金, XLG2 被发现与 AGB1 交往,在 Arabidopsis 的唯一的 G 子单元,它以前被发现了是在到 necrotrophic 的抵抗的一个积极管理者真菌的病原体。然而,没有重要差别在三 xlg 单身者异种之间被发现, xlg2 xlg3 在到 necrotrophic 的抵抗的两倍异种, xlg 三元组异种,和野类型的植物真菌的病原体 Botrytis cinerea 或 Alternaria brassicicola。这些结果建议 XLG2 和 AGB1 是与原型的 heterotrimeric G 蛋白质不同的 G 蛋白质建筑群的部件并且可以在 modulating 防卫回答有不同功能。 | Huifen Zhu Guo-Jing Li Lei Ding Xiangqin Cui Howard Berg Sarah M. Assmann Yiji Xia | 2009 | Molecular Plant2009，2，3： | 8 |
| 2 | Hybrid copper-cobalt hexacyanoferrate films modified glassy carbon electrode as an electrochemical sensor for hydrogen peroxide 显示文摘 | Cui Xingpin Lin Xiangqin | 2002 | Analytical Letters2002，35，4： | 1 |
| 3 | CRISPR/Cas9 therapeutics:progress and prospects显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene-editing technology is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-causing genes with great precision and efficiency.A variety of efficient Cas9 variants and derivatives have been developed to cope with the complex genomic changes that occur during diseases.However,strategies to effectively deliver the CRISPR system to diseased cells in vivo are currently lacking,and nonviral vectors with target recognition functions may be the focus of future research.Pathological and physiological changes resulting from disease onset are expected to serve as identifying factors for targeted delivery or targets for gene editing.Diseases are both varied and complex,and the choice of appropriate gene-editing methods and delivery vectors for different diseases is important.Meanwhile,there are still many potential challenges identified when targeting delivery of CRISPR/Cas9 technology for disease treatment.This paper reviews the current developments in three aspects,namely,gene-editing type,delivery vector,and disease characteristics.Additionally,this paper summarizes successful examples of clinical trials and finally describes possible problems associated with current CRISPR applications. | Tianxiang Li Yanyan Yang Hongzhao Qi Weigang Cui Lin Zhang Xiuxiu Fu Xiangqin He Meixin Liu Pei-feng Li Tao Yu | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 0 |
| 4 | Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor显示文摘Large-scale expansion of the osteoblasts of a Sprague-Dawley(SD)rat was studied in a rotating wall hollow-fiber membrane bioreactor(RWHMB)by using hollow-fiber membrane as the carrier.For the sake of contrast,cells were also expanded in a T-flask using a hollow-fiber membrane as carrier and in a rotating wall vessel bioreactor(RWVB)using a microcarrier.During the culture period,the cells were sampled every 12 h,and after 5 days,the cells were harvested and evaluated with scanning electron microscopy(SEM),hematoxylin-eosin(HE)staining and alkaline phosphatase(ALP)staining.Moreover,von-Kossa staining and Alizarin Red S stain-ing were carried out for mineralized nodules formation.The results show that in RWHMB,the cells present better morphology and vitality and secrete much more extracel-lular matrix.It is concluded that the RWHMB combines the advantages of the rotating wall vessel and hollow-fiber membrane bioreactors.The hydrodynamic stimulation within it accelerates the metabolism of the osteoblast and mass transfer,which is propitious to cell differenti-ation and proliferation. | Kedong SONG Tianqing LIU Hu ZHAO Xiangqin LI Zhanfeng CUI Xuehu MA | 2008 | Frontiers of Chemical Science and Engineering2008，2，1： | 0 |
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