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| 1 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 2 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 3 | IntegrinαEβ7^(+)T cells direct intestinal stem cell fate decisions via adhesion signaling显示文摘Intestinal stem cell(ISC)differentiation is regulated precisely by a niche in the crypt,where lymphocytes may interact with stem and transient amplifying(TA)cells.However,whether and how lymphocyte–stem/TA cell contact affects ISC differentiation is largely unknown.Here,we uncover a novel role of T cell–stem/TA cell contact in ISC fate decisions.We show that intestinal lymphocyte depletion results in skewed ISC differentiation in mice,which can be rescued by T cell transfer.Mechanistically,integrinαEβ7 expressed on T cells binds to E-cadherin on ISCs and TA cells,triggering E-cadherin endocytosis and the consequent Wnt and Notch signaling alterations.BlockingαEβ7−E-cadherin adhesion suppresses Wnt signaling and promotes Notch signaling in ISCs and TA cells,leading to defective ISC differentiation.Thus,αEβ7^(+)T cells regulate ISC differentiation at single-cell level through cell–cell contact-mediatedαEβ7−E-cadherin adhesion signaling,highlighting a critical role of the T cell–stem/TA cell contact in maintaining intestinal homeostasis. | Shiyang Chen Yajuan Zheng Xiaojuan Ran Hui Du Hua Feng Lei Yang Yating Wen Changdong Lin Shihui Wang Mengwen Huang Zhanjun Yan Dianqing Wu Hongyan Wang Gaoxiang Ge An Zeng Yi Arial Zeng Jianfeng Chen | 2021 | Cell Research2021，31，12： | 3 |
| 4 | Comparative analysis of symbiotic phenotypes of soybean ’’Tianlong 1’’ with two different rhizobia and co-inoculation at different developmental stages显示文摘Symbiotic nitrogen fixation between soybean and rhizobia, which directly catalyze reduction of atmospheric nitrogen into ammonia, has critical importance in agricultural economy and environment. However, soybean symbiotic nitrogen efficiency, which changes with growth stage, largely relies on symbiotic host-specificity and nodule development. In this report, symbiotic phenotypes of soybean ‘Tianlong 1’ with different inoculation (inoculated with Bradyrhizobium japonicum strain 113-2, Sinorhizobium fredii USDA205 and co-inoculated with 113-2 and USDA205) were comparatively studied at different developmental stages. Without additional nitrogen, chlorophyll content, above ground fresh weight,plant height, nodule number and nodule dry weight changed with different rhizobia at various developmental stages. 113-2 had more advantages than USDA205 in promoting plant growth and nodulation. Nodulation morphology of co-inoculation was more similar to 113-2 than USDA205. Competitive nodulation between two rhizobia promoted plant growth, which had significant agricultural application prospect. For the first time, this study provided evidence for co-inoculation with different rhizobia having positive effect on plant growth, and shed new light on the competitive relationship between different rhizobia. | Rong Li Songli Yuan Haifeng Chen Chanjuan Zhang Limiao Chen Qingnan Hao Xiaojuan Zhang Zhihui Shan Zhonglu Yang Shuilian Chen Dezhen Qiu Xin’an Zhou | 2017 | Oil Crop Science2017，2，3： | 1 |
| 5 | NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma显示文摘Multiple myeloma(MM) is still an incurable hematologic malignancy, which is eagerly to the discovery of novel therapeutic targets and methods. N-acetyltransferase 10(NAT10) is the first reported regulator of mRNA acetylation that is activated in many cancers. However, the function of NAT10 in MM remains unclear. We found significant upregulation of NAT10 in MM patients compared to normal plasma cells, which was also highly correlated with MM poor outcome. Further enforced NAT10 expression promoted MM growth in vitro and in vivo, while knockdown of NAT10 reversed those effects. The correlation analysis of acetylated RNA immunoprecipitation sequencing(ac RIP-seq) and ribosome profiling sequencing(Ribo-seq) combined with RIP-PCR tests identified centrosomal protein 170(CEP170) as an important downstream target of NAT10. Interfering CEP170 expression in NAT10-OE cells attenuated the acceleration of cellular growth caused by elevated NAT10. Moreover,CEP170 overexpression promoted cellular proliferation and chromosomal instability(CIN) in MM.Intriguingly, remodelin, a selective NAT10 inhibitor, suppressed MM cellular growth, induced cellular apoptosis in vitro and prolonged the survival of 5TMM3VT mice in vivo. Collectively, our data indicate that NAT10 acetylates CEP170 mRNA to enhance CEP170 translation efficiency, which suggests that NAT10 may serve as a promising therapeutic target in MM. | Rongfang Wei Xing Cui Jie Min Zigen Lin Yanyan Zhou Mengjie Guo Xiaojuan An Hao Liu Siegfried Janz Chunyan Gu Hongbo Wang Ye Yang | 2022 | Acta Pharmaceutica Sinica B2022，12，8： | 1 |
| 6 | Herbivore defense responses and associated herbivore defense mechanism as revealed by comparing a resistant wild soybean with a susceptible cultivar显示文摘Plants have evolved sophisticated defense mechanisms against herbivores to help them adapt to the environment. Understanding the defense mechanisms in plants can help us control insects in a more effective manner. In this study, we found that compared with Tianlong 2(a cultivated soybean with insect susceptibility), ED059(a wild soybean line with insect resistance)contains sharper pubescence tips, as well as lower transcript levels of wound-induced protein kinase(WIPK) and salicylic acid-induced protein kinase(SIPK), which are important mitogen-activated protein kinases involved in early defense response to herbivores. The observed lower transcript levels of WIPK and SIPK induced higher levels of jasmonic acid(JA), JA biosynthesis enzymes(AOC3) and some secondary metabolites in ED059. Functional analysis of the KTI1 gene via Agrobacterium-mediated transformation in Arabidopsis thaliana indicated that it plays an important role in herbivore defense in ED059. We further investigated the molecular response of third-instar Helicoverpa armigera(Hübner) larvae to Tianlong 2 and ED059. We found apoptotic cells only in the midguts of larvae that fed on ED059. Compared with larvae reared on the susceptible cultivar Tianlong 2, transcript levels of catalase(CAT) and glutathione S-transferase(GST) were up-regulated, whereas those of CAR, CHSB, and TRY were down-regulated in larvae that fed on the highly resistant variety ED059. We propose that these differences underlie the different herbivore defense responses of ED059 and Tianlong 2. | Xiaoyi Wang Haifeng Chen Zhihui Shan Qingnan Hao Chanjuan Zhang Zhonglu Yang Xiaojuan Zhang Songli Yuan Dezhen Qiu Shuilian Chen Yongqin Jiao Xin'an Zhou | 2015 | The Crop Journal2015，3，6： | 1 |
| 7 | Symbiotic matching between soybean ‘Tianlong 1’ and rhizobia显示文摘‘Tianlong 1’ is widely popularized and applied with good comprehensive characters and is also good receptor for genetic transformation. However, little is known about symbiotic nitrogen fixation of ‘Tianlong 1’. In this report, 10 soybean rhizobia from different areas were selected to perform symbiotic matching test with ‘Tianlong 1’ in chamber. Two aboveground indicators (fresh weight aboveground and plant height) and 4 underground indicators (length of root, number of nodules, dry weight of root nodules per plant and morphology of nodules) were measured and data were statistically analyzed. Results showed that ‘Tianlong 1’ was a broad-hosted soybean which has different symbiotic matching abilities with these 10 rhizobia. Bradyrhizobium japonicum has more advantages than Sinorhizobium fredii in promoting plant growth and nodulation. 113-2 and 5036 were high effective strains. 113-2 was regarded as the most effective rhizobium matching to ‘Tianlong 1’ due to geographical advantages and adaptability. This research will promote symbiotic fixation of ‘Tianlong 1’ in agricultural industry for the first time. | Rong Li Songli Yuan Haifeng Chen Chanjuan Zhang Limiao Chen Qingnan Hao Xiaojuan Zhang Zhihui Shan Zhonglu Yang Shuilian Chen Dezhen Qiu Xin’an Zhou | 2017 | Oil Crop Science2017，2，2： | 1 |
| 8 | Pedigree analysis of nationally approved soybean cultivars from 1984 to 2013 and implications for breeding显示文摘 | Zhonglu Yang Xiaomin Guan Yanqin He Zhihui Shan Dezhen Qiu Xiaojuan Zhang Limiao Chen Chanjuan Zhang Songli Yuan Shuilian Chen Qingnan Hao Haifeng Chen Xin'an Zhou | 2016 | Oil Crop Science2016，1，4： | 0 |
| 9 | 坏死激酶RIP1/RIP3通过特异性结合HSV-1的ICP6蛋白从而启动细胞坏死来抑制病毒在小鼠中的繁殖显示文摘2007年3月,细胞出版社正式推出《细胞-宿主与微生物》(月刊)。本刊出版的研究论文和综述文章重点关注对微生物与其宿主关系的理解。通过与作者、审稿人和科学编委的紧密合作,本刊的编辑人员以保持细胞出版社期刊一贯的高标准为使命,对该领域的研究结果予以及时报道。该杂志2015年公布的影响因子为12.328。 | Zhe Huang Su-Qin Wu 梁耀极 Xiaojuan Zhou Wanze Chen Lisheng Li Jianfeng Wu Qiuyu Zhuang Chang'an Chen Jingxian Li Chuan-Qi Zhong Weixiang Xia Rongbin Zhou Chunfu Zheng 韩家淮 | 2016 | 科学新闻2016，0，1： | 0 |
| 10 | Improving the colloidal stability of Cellulose nano-crystals by surface chemical grafting with polyacrylic acid显示文摘Cellulose nano-crystals(CNC)can be tailored for various value-added applications.However,its use in aqueous systems is hampered by its limited dispersability,especially at a high CNC concentration.In this study,the improvement of CNC colloidal stability by surface chemical grafting with polyacrylic acid(PAA)was investigated,and the zeta potential and the charge density of the chemically modified CNC were analyzed.The results showed that an acrylic dosage of 1%(based on the dry weight of CNC)was sufficient to significantly enhance the colloidal stability.CNC,after chemical grafting with PAA,showed better stability against the increase in storage time or solid content of the aqueous medium,compared with the un-modified CNC. | Dong Cheng Yangbing Wen Xingye An Xuhai Zhu Xiaojuan Cheng Linqiang Zheng Joseph E Nasrallah | 2016 | Journal of Bioresources and Bioproducts2016，1，3： | 0 |
| 11 | Evaluation of seed vigor in soybean germplasms from different eco-regions显示文摘Seed vigor plays an essential role in soybean production.Soybean seed is sensitive to environment and easy to get seed deterioration.Generally soybean seed vigor can be maintained for less than one year and have to be multiplied every year.Varieties with good seed vigor are essential for maintaining planting population and stable yield.In this research,419 germplasms from three eco-regions,South China,Huanghuai region and Northeast China,were evaluated for seed vigor by assessing germination ratio,germination potential and germination index.About 34.8%of tested germplasm had germination ratio equal or more than 85%,suggested by the national soybean seed quality standard of China.Only 9 from 82 tested cultivars met the seed standard.Seed germination ratio distribution had obvious regional feature.Landraces performed better seed vigor than cultivars.Germplasms from South China had stronger seed vigor compared to those from Huanghuai and Northeast regions.Seed vigor has no significant relationship with maturity and 100-seed weight.Totally 21 germplasms with strong seed vigor,favor maturation and seed size were identified and can be used in future breeding program. | Qingnan Hao Yanyan Yang Changxun Guo Xuefei Liu Haifeng Chen Zhonglu Yang Chanjuan Zhang Limiao Chen Songli Yuan Shuilian Chen Dong Cao Wei Guo Dezhen Qiu Xiaojuan Zhang Zhihui Shan Xin’an Zhou | 2020 | Oil Crop Science2020，5，1： | 0 |
| 12 | Corrigendum to “The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs” [Genes & Diseases 5 (2018) 62–74]显示文摘The authors regret having an image assembly error in Figure 5Ca,in which the image for the 'Oh dBiFP-AdRFp'group was erroneously duplicated with an overlapping image from the'36h BiFP dBIFP-AdR-simH19'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C. Haydon Hue H. Luu Lan Zhou Russell R. Reid Tong-Chuan He Xingye Wu | 2023 | Genes & Diseases2023，10，2： | 0 |
| 13 | Corrigendum to “Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference” [Genes & Diseases 5(2018):172–184]显示文摘The authors regret having several image assembly errors.Specifically,in Figure 3A panel b,the image for 'AdsimB9-4 only'group was erroneously duplicated with an overlapping image from the'AdRFp'group;and the image for'AdsimB9-1+BMP9'groupwas erroneouslyduplicatedwithan overlapping image from'AdsimB9-8+BMP9'group.In Figure 4Apanel a,the images for'BMP9'group and 'BMP9+simB9-4'group were erroneously duplicated with an overlapping image from'simB9-4'group.In Figure 5A,the image for'BMP9+simB9-4/Day3'group was erroneously duplicated with an overlapping image from'BMP9+simB9-7/Day3'group;and the image for'BMP9+simB9-4/Day5'group was erroneously duplicated with an overlapping image from an unrelated experiment.In Figure 6B,the image for'BMP9+simB9-7/Day 11'group was erroneously duplicated with an overlapping image from the'BMP9+simB9-4/Day 11'group. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C. Haydon Michael J. Lee Russell R. Reid Jennifer Moriatis Wolf Qiong Shi Hue H. Luu Tong-Chuan He Yaguang Weng | 2023 | Genes & Diseases2023，10，2： | 0 |
| 14 | PtrDJ1C,an atypical member of the DJ-1 superfamily,is essential for early chloroplast development and lignin deposition in poplar显示文摘The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast biogenesis.However,many questions regarding these processes remain unanswered,particularly in perennial woody plants.As a model material of woody plants,poplar not only has very significant value of research,but also possesses economic and ecological properties.This study reports the Populus trichocarpa DJ-1C(PtrDJ1C)factor,encoded by a nuclear gene,and a member of the DJ-1 superfamily.PtrDJ1C knock-out with the CRISPR/Cas9 system resulted in different albino phenotypes.Chlorophyll fluorescence and immunoblot analyses showed that the levels of photosynthetic complex proteins decreased significantly.Moreover,the transcript level of plastid-encoded RNA polymerase-dependent genes and the splicing efficiency of several introns were affected in the mutant line.Furthermore,rRNA accumulation was abnormal,leading to developmental defects in chloroplasts and affecting lignin accumulation.We concluded that the PtrDJ1C protein is essential for early chloroplast development and lignin deposition in poplar. | Xinwei Wang Chunxue Shao Libo Liu Yue Wang Yaqi An Hui Li Yunwei Ding Yanping Jing Xiaojuan Li Jianwei Xiao | 2023 | Horticultural Plant Journal2023，9，5： | 0 |
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