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| 1 | Analysis of Gene Expression in the K562-n High Tumorigenitic Human Leukemia Cell Line显示文摘OBJECTIVE The human leukemia K562-n cell line displays much higher tumorigenic actively in nude mice compared with its parental K562 cell line. The molecular mechanism of the differences in tumorigenicity between K562-n and K562 in nude mice was examined.METHODS The differences in gene expression between K562 and K562-n cells were analyzed by using cDNA microarrays.RESULTS Among the 12,800 genes examined, there was a significant difference in expression of 139 genes between K562-n and K562 cells.Eighty-five of these genes have been registered in the GeneBank and 54 are unknown. The genes accessible from the GeneBank include:l) oncogenes and tumor-supressor genes; 2) genes related to transcription regulation, the cell cycle and apoptosis; 3) genes related to the cytoskeleton and cytokinetics; 4) genes related to metabolism and transport; 5) genes related to immune function. There were also some differently expressed genes with mixed functions.CONCLUSION There are many genes differentially expressed between K562-n and K562 cells .The high tumorigenicity of the human leukemia K562-n cell line in nude mice might be related to its specific geneexpression profile. | ShuqingLü XiaopingXu FangXia JianMinWang | 2005 | Chinese Journal of Clinical Oncology2005，2，1： | 0 |
| 2 | Clinical and Pathological Features of Primary Gastrointestinal Non-Hodgkin's Lymphoma显示文摘OBJECTIVE The study was initiated to obtain histologic distribution, clinical features, and treatment results in patients with primary gastrointestinal non-Hodgkin's lymphomas.METHODS Between January 1990 and January 2000, 89 PGI NHL patients were eligible to evaluate clinical features. Histological and immunohistological studies were routinely used and all the specimens were reclassified according to the recently published WHO classification system.RESULTS (1)Clinically, among the 89 patients, there were 24 patients in stage IE,33 in stage IIE,19 in stage IIIE,and 13 in stage IVE. (2)Immunohistological studies revealed 72 patients were with B-cell type and only 17 with T-cell type. (3)Altogether, 15 MALT lymphoma were diagnosed among 89 PGI NHL patients, and 14/15 were found primary in the stomach.(4)The 3-year and 5-year overall survival were 77.0% (57/74) and 53.6% (30/56)for the total group.CONCLUSION No clinical symptoms and signs were found to be specific for the diagnosis of PGI NHL. Most patients were in stage IE and liE when diagnosed and the intermediate grade and B-cell type were more common than the others. Surgical resection of the tumor and standard combined chemotherapy post surgery were suggested to be the most effective measures for the long term survival of the PGI NHL patients. | KangzhiWu JianminYang DaliMa XionminSong WeipingZhang XiaopingXu BiheMin JianminWang | 2004 | Chinese Journal of Clinical Oncology2004，1，5： | 0 |
| 3 | The Synergistic Inhibitory Effect of STI571 in Combination with Arsenic Trioxide (As2O3) on Multidrug-Resistant Leukemic Cells显示文摘OBJECTIVE To study the synergistic effect of STI571, an inhibitor of tyrosine kinase, in combination with arsenic trioxide As2O3 on a multidrug-resistant leukemia cell line expressing bcr-abl. METNODS The cytotoxic effect of STI571 alone or in combination with different concentrations of As2O3 on the bcr-abl and mdrl -positive leukemia cell line, K562-n/VCR, was examined by the MTT method. RESULTS One μmol/L of STI571 alone had no significant cytotoxic effect on K562-n/VCR cells. However the cytotoxic effect increased markedly when combined with As2O3 at concentrations of 10^-5, 10^-6, 10^-7 and 10^-8 mol/L. The IC50 of K562-n/VCR cells in As2O3 group was 1.879 μmol/L, with. Upon addition of STI571, the IC50 decreased to 0.155 μmol/L resulting in a synergistic cytotoxic effect on K562-n/VCR cells that was increased 12.1 times. CONCLUSION A combination of STI571 with As2O3 has a more powerful inhibitory effect on leukemia cells expressing positive bcr-abl and positive mdrl compared to the effect with As2O3 alone. | LiChen JianminWang XiaopingXu LeiGao XinhongFei JingweiLou ZhengxiaHuang | 2005 | Chinese Journal of Clinical Oncology2005，2，3： | 0 |
| 4 | 孔隙度对液相烧结钨重合金变表的影响显示文摘试验研究了液相烧结孔隙度对变形的影响,将含钨从78%至93%(重量百分比)的W-Ni-Fe合金在1500℃进行液相烧结30分钟,结果表明孔隙度对变形没有明显影响,烧结后,高孔隙低孔隙度的压坯表现出相似的变形外貌,但另一方面,变形丰固液比的提高而减小,含钨达93%的试样却不再产生变形,淬火试验表明,试样在致密化将近完成时方开始变形。由于液体上析出,在液相含量高的试样中,变形在熔体形成后几分钟内产生,这些结论与German的模型预测结果一致,对烧结显微组织进行了定量测试以确定固相体积分数和邻接度,生坯孔隙度对显微组织的演变没有明显影响。 | XiaopingXu 黄键 | 2000 | 国外难熔金属与硬质材料2000，16，1： | 0 |
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