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| 1 | Draft genome sequence of cauliflower(Brassica oleracea L.var.botrytis)provides new insights into the C genome in Brassica species显示文摘Cauliflower is an important variety of Brassica oleracea and is planted worldwide.Here,the high-quality genome sequence of cauliflower was reported.The assembled cauliflower genome was 584.60 Mb in size,with a contig N50 of 2.11 Mb,and contained 47,772 genes;56.65%of the genome was composed of repetitive sequences.Among these sequences,long terminal repeats(LTRs)were the most abundant(32.71%of the genome),followed by transposable elements(TEs)(12.62%).Comparative genomic analysis confirmed that after an ancient paleohexaploidy(γ)event,cauliflower underwent two whole-genome duplication(WGD)events shared with Arabidopsis and an additional whole-genome triplication(WGT)event shared with other Brassica species.The present cultivated cauliflower diverged from the ancestral B.oleracea species~3.0 million years ago(Mya).The speciation of cauliflower(~2.0 Mya)was later than that of B.oleracea L.var.capitata(approximately 2.6 Mya)and other Brassica species(over 2.0 Mya).Chromosome no.03 of cauliflower shared the most syntenic blocks with the A,B,and C genomes of Brassica species and its eight other chromosomes,implying that chromosome no.03 might be the most ancient one in the cauliflower genome,which was consistent with the chromosome being inherited from the common ancestor of Brassica species.In addition,2,718 specific genes,228 expanded genes,2 contracted genes,and 1,065 positively selected genes in cauliflower were identified and functionally annotated.These findings provide new insights into the genomic diversity of Brassica species and serve as a valuable reference for molecular breeding of cauliflower. | Deling Sun Chunguo Wang Xiaoli Zhang Wenlin Zhang Hanmin Jiang Xingwei Yao Lili Liu Zhenghua Wen Guobao Niu Xiaozheng Shan | 2019 | Horticulture Research2019,6,1: | 6 |
| 2 | Analyses of landuse change impacts on catchment runoff using different time indicators based on SWAT model显示文摘 | Bingqing Lin Xingwei Chen Huaxia Yao | 2015 | Ecological Indicators2015,58,: | 1 |
| 3 | Multi‑omics reveals that the host‑microbiome metabolism crosstalk of differential rumen bacterial enterotypes can regulate the milk protein synthesis of dairy cows显示文摘Background Dairy cows’lactation performance is the outcome of the crosstalk between ruminal microbial metabo-lism and host metabolism.However,it is still unclear to what extent the rumen microbiome and its metabolites,as well as the host metabolism,contribute to regulating the milk protein yield(MPY).Methods The rumen fluid,serum and milk of 12 Holstein cows with the same diet(45%coarseness ratio),parity(2–3 fetuses)and lactation days(120–150 d)were used for the microbiome and metabolome analysis.Rumen metabolism(rumen metabolome)and host metabolism(blood and milk metabolome)were connected using a weighted gene co-expression network(WGCNA)and the structural equation model(SEM)analyses.Results Two different ruminal enterotypes,with abundant Prevotella and Ruminococcus,were identified as type1 and type2.Of these,a higher MPY was found in cows with ruminal type2.Interestingly,[Ruminococcus]gauvreauii group and norank_f_Ruminococcaceae(the differential bacteria)were the hub genera of the network.In addition,differential ruminal,serum and milk metabolome between enterotypes were identified,where the cows with type2 had higher L-tyrosine of rumen,ornithine and L-tryptophan of serum,and tetrahydroneopterin,palmitoyl-L-carnitine,S-lactoylglutathione of milk,which could provide more energy and substrate for MPY.Further,based on the identi-fied modules of ruminal microbiome,as well as ruminal serum and milk metabolome using WGCNA,the SEM analysis indicated that the key ruminal microbial module1,which contains the hub genera of the network([Ruminococcus]gauvreauii group and norank_f_Ruminococcaceae)and high abundance of bacteria(Prevotella and Ruminococcus),could regulate the MPY by module7 of rumen,module2 of blood,and module7 of milk,which contained L-tyrosine and L-tryptophan.Therefore,in order to more clearly reveal the process of rumen bacterial regulation of MPY,we established the path of SEM based on the L-tyrosine,L-tryptophan and related components.The SEM based on the metabolites suggested that[Ruminococcus]gauvreauii group could inhibit the energy supply of serum tryptophan to MPY by milk S-lactoylglutathione,which could enhance pyruvate metabolism.Norank_f_Ruminococcaceae could increase the ruminal L-tyrosine,which could provide the substrate for MPY.Conclusion Our results indicated that the represented enterotype genera of Prevotella and Ruminococcus,and the hub genera of[Ruminococcus]gauvreauii group and norank_f_Ruminococcaceae could regulate milk protein synthesis by affecting the ruminal L-tyrosine and L-tryptophan.Moreover,the combined analysis of enterotype,WGCNA and SEM could be used to connect rumen microbial metabolism with host metabolism,which provides a fundamental understanding of the crosstalk between host and microorganisms in regulating the synthesis of milk composition. | Chenguang Zhang Mengya Wang Huifeng Liu Xingwei Jiang Xiaodong Chen Tao Liu Qingyan Yin Yue Wang Lu Deng Junhu Yao Shengru Wu | 2023 | Journal of Animal Science and Biotechnology2023,14,6: | 1 |
| 4 | Corrosion inhibition of carbon steel by polyaniline nanofibers显示文摘 | Bin Yao Gengchao Wang Jiankun Ye Xingwei Li | 2007 | Materials Letters2007,,12: | 1 |
| 5 | Routing security scheme based on reputation evaluation in hierarchical ad hoc networks显示文摘 | Yao Yu Lei Guo Xingwei Wang Cuixiang Liu | 2009 | Computer Networks2009,,9: | 1 |
| 6 | Analyses of landuse change impacts on catchment runoff using different time indicators based on SWAT model 显示文摘 | Lin Bingqing Chen Xingwei Yao Huaxia | 2015 | Ecological Indicators2015,58,: | 1 |
| 7 | A high-precision borehole gravimeter显示文摘The paper describes the working principle and structure of a newly designed high-precision (micro-gal) gravimeter, which is basically a vertically suspending spring-weight system, using a highly sensitive capacitance sensor of movement in a controlled temperature environment in a borehole and a multi-frequency output for both earth-tide and earthquake monitoring. The preliminary test indicated that, while in a stabilization process, it had met all the technical requirements for a relative gravimeter used for earth tide and earthquake monitoring. | Li Jiaming Yao Zhigui Wen Xingwei Gong Ping Wei Jin | 2011 | Geodesy and Geodynamics2011,2,3: | 0 |