| 1 | Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters显示文摘The hepatitis B virus(HBV) is a global public health challenge due to its highly contagious nature. It is estimated that almost 300 million people live with chronic HBV infection annually. Although nucleoside analogs markedly reduce the risk of liver disease progression, the analogs do not fully eradicate the virus. As such, new treatment options and drugs are urgently needed. Psoralen is a nourishing monomer of Chinese herb and is known to inhibit virus replication and inactivate viruses. In this study, we evaluated the potential of psoralen as an anti-HBV agent.Quantitative PCR and Southern blot analysis revealed that psoralen inhibited HBV replication in Hep G2.2.15 cells in a concentration-dependent manner. Moreover, psoralen was also active against the 3TC/ETV-dual-resistant HBV mutant. Further investigations revealed that psoralen suppressed both HBV RNA transcription and core protein expression. The transcription factor FOXO1, a known target for PGC1α co-activation, binds to HBV precore/core promoter enhancer II region and activates HBV RNA transcription. Co-immunoprecipitation showed that psoralen suppressed the expression of FOXO1, thereby decreasing the binding of FOXO1 co-activator PGC1αto the HBV promoter. Overall, our results demonstrate that psoralen suppresses HBV RNA transcription by downregulating the expression of FOXO1 resulting in a reduction of HBV replication. | Xinna Ma Heng Li Ying Gong Feifei Liu Xiankun Tong Fenghua Zhu Xiaoqian Yang Li Yang Jianping Zuo | 2022 | Virologica Sinica2022，37，2： | 2 |
| 2 | Clustering and Differentiation of glr-3 Gene Function and Its Homologous Proteins显示文摘In order to adapt to the low temperature environment,organisms transmit excitement to the central system through the thermal sensing system,which is a classic reflex reaction.The cold receptor GLR-3 perceives cold and produces cold avoidance behavior through peripheral sensory neurons ASER.In order to further understand the gene encoding of the cold sensing glr-3 gene and the evolution of its homologous gene group function and protein function,the nucleotide sequence and amino acid sequence of the glr-3 gene and its homologous gene in 24 species were obtained and compared.By clustering with the GRIK2 gene sequence of Rana chensinensis,the bioinformatics method was used to predict and sequence analyze the change of gene,evolution rate,physical and chemical properties of protein,glycosylation sites,phosphorylation sites,secondary structure and tertiary structure of protein.The analysis results show that the glr-3 gene and its homologous gene have obvious positive selection effect.The protein prediction analysis showed that the glr-3 gene and its homologous genes encoded proteins in these 25 species were hydrophilic proteins,and the proportion of side chains of aliphatic amino acids was high.The transmembrane helix was widespread and there were more N-glycosylation sites and O-glycosylation sites.The protein phosphorylation sites encoded were serine,threonine and tyrosine phosphorylation sites.Secondary structure prediction showed that the secondary structure units of the encoded protein wereα-helix,β-turn,random coil and extended chain,and the proportion ofα-helix was the largest.This study provides useful information on the evolution and function of the cold sensing gene glr-3 and its homologous genes. | Yue Ma Tiantian Guo Yihe Wang Xinna Li Jingyu Zhang | 2021 | Journal of Zoological Research2021，3，3： | 0 |
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