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| 1 | Electrochemical Synthesis of Polythiophene in an Ionic Liquid显示文摘Polythiophene (PTh) was prepared by the direct electrochemical synthesis in an ionic liquid ([BMIM]PF6) containing 0.1 mol/L thiophene by cyclic voltammetry, constant potential and constant current techniques. It is found that smooth and bluegreen PTh films can be obtained at a potential of ca. +1.75 V ( vs. Ag/AgCl ) or a current of ca. 1.5 mA cm-2 in the ionic liquid. | JiaHuaSHI XunSUN 等 | 2002 | Chinese Chemical Letters2002，13，4： | 4 |
| 2 | Preparation and characteristics of DNA-nanoparticles targeting to hepatocarcinoma cells显示文摘AIM:To prepare thymidine kinase gene (TK gene) nanopartides and to investigate the expression of TK gene.METHODS: Poly(D,L-lactic-co-glycolic acid) (PLGA), a biodegradable and biocompatible polymer, was used to prepare recombinant plasmid p^EGFP-AFP nanoparticles by a double-emulsion evaporation technique. Characteristics of the nanoparticles were investigated in this study, including morphology, entrapment efficiency, and tissue distribution.The expression of TK gene was also investigated by MTT assay, by which the viable cells were determined alter the addition of ganciclovir (GCV).The enhanced green fluorescent protein (EGFP) expression in human hepatocellular carcinoma SMMC-7721 cells and normal parenchymal Chang liver cellswere assessed by flow cytometry.RESULTS: The prepared plasmid-nanoparticles had regular spherical surface and narrow particle size span with a mean diameter of 72±12nm.The mean entrapment efficiency was 91.25%. A total of 80.14% DNA was found to be localized in the livers after 1-h injection with ^32P-DNA-PLGA nanoparticles in mouse caudal vein. The expression of DNA encapsulated in nanopartides was much higher than that in naked DNA, and human hepatocellular carcinoma SMMC-7721 cells were more sensitive to GCV than human normal parenchymal Chang liver cells.CONCLUSION:The enhanced transfection efficiency and stronger ability to protect plasmid DNA from being degraded by nucleases are due to nanoparticles encapsulation. | QinHe JiLiu XunSun Zhi-RongZhang | 2004 | World Journal of Gastroenterology2004，10，5： | 4 |
| 3 | Transfection efficiency of pORF lacZ plasmid lipopolyplex to hepatocytes and hepatoma cells显示文摘AIM:To develop a novel non-viral gene delivery system,which has a small particle size and a high transfection efficiency to hepatocyte and hepatoma cells.METHODS:Lipid-polycation-DNA lipopolyplex (LPD) was prepared by mixing plasmid DNA and polylysine. The resulted polyplex was incubated for 10min at room temperature,following the addition of preformed cationic liposomes. The morphology of LPD was observed by transmission electron microscopy.The diameter and surface charge of LPD were measured by photon correlation spectroscopy (PCS). The nuclease protection ability of LPD was evaluated by agarose gel electrophoresis. Estimation of the transfection efficiency was performed by galactosidase assay in Chang cells and SMMC-7721 cells.RESULTS:LPD had a regular spherical surface. The average diameter and the zeta potential of LPD were 132.1nm and 26.8mV respectively. LPD could protect plasmid DNA from nuclease degradation after 2 hours incubation at 37℃ while the naked DNA degraded rapidly. The average transfection efficiencies were 86.2±8.9% and 72.4±6.5% in Chang cells and SMMC-7721 cells respectively.CONCLUSION: LPD has a rather small particle size and a high transfection activity. LPD may be a good non-viral vector for application in some gene delivery. | XunSun Hong-WeiZhang Zhi-RongZhang | 2004 | World Journal of Gastroenterology2004，10，4： | 4 |
| 4 | Expression of interferon-alpha/beta receptor protein in liver of patients with hepatitis C virus-related chronic liver disease显示文摘AIM: To study the expression of interferon-alpha/beta (IFN-α/β) receptor protein in liver of patients with hepatitis C virus (HCV)-related chronic liver disease and its clinical significance.METHODS: A total of 181 patients with HCV-related chronic liver disease included 56 with HCV-related liver cirrhosis (LC) and 125 with chronic hepatitis C (CHC).CHC patients were treated with five megaunits of interferon-α1b six times weekly for the first 2 weeks and then every other day for 22 wk. The patients were divided into interferon (IFN) treatment-responsive and nonresponsive groups, but 36 patients lost follow-up shortly after receiving the treatment. The expression of IFN-α/β receptor (IFN-α/βR) protein in liver of all patients was determined with immunofluorescence.RESULTS: In liver of patients with HCV-related chronic liver disease, the expression of IFN-α/βR protein in liver cell membrane was stronger than that in cytoplasm and more obvious in the surroundings of portal vein than in the surroundings of central vein. Moreover, it was poorly distributed in hepatic Iobules. The weak positive, positive and strong positive expression of IFN-α/βR were 40% (50/125), 28% (35/125), 32% (40/125), respectively in CHC group, and 91.1% (51/56), 5.35% (3/56), and 3.56% (2/56), respectively in LC group. The positive and strong positive rates were higher in CHC group than in LC group (P<0.01). In IFN treatment responsive group, 27.8% (10/36) showed weak positive expression; 72.2% (26/36)showed positive or strong positive expression. In the nonresponsive group, 71.7% (38/53) showed weak positive expression; 28.3% (15/53) showed positive or strong positive expression. The expression of IFN-α/βR protein in liver was more obvious in IFN treatment responsive group than in non-responsive group.CONCLUSION: Expression of IFN-α/βR protein in liver of patients with HCV-related chronic liver disease is likely involved in the response to IFN treatment. | Xiang-WeiMeng Bao-RongChi Li-GangChen Ling-LingZhang YanZhuang Hai-YanHuang XunSun | 2005 | World Journal of Gastroenterology2005，11，25： | 3 |
| 5 | The effect of cell cycle and ex- pression of cyclin B1 and cyclin C protein in hepatocellular carci noma cell line HepG2 and SMMC 7721 after of Silencing :-cate- nin gene显示文摘 | Wang B Xunsun Liu JY | 2012 | Hepatogastroenterology2012，59，114： | 1 |
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