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| 1 | Heat shock protein 20 promotes sirtuin 1-dependent cell proliferation in induced pluripotent stem cells显示文摘BACKGROUND Heat shock proteins(HSPs)are molecular chaperones that protect cells against cellular stresses or injury.However,it has been increasingly recognized that they also play crucial roles in regulating fundamental cellular processes.HSP20 has been implicated in cell proliferation,but conflicting studies have shown that it can either promote or suppress proliferation.The underlying mechanisms by which HSP20 regulates cell proliferation and pluripotency remain unexplored.While the effect of HSP20 on cell proliferation has been recognized,its role in inducing pluripotency in human-induced pluripotent stem cells(iPSCs)has not been addressed.AIM To evaluate the efficacy of HSP20 overexpression in human iPSCs and evaluate the ability to promote cell proliferation.The purpose of this study was to investigate whether overexpression of HSP20 in iPSCs can increase pluripotency and regeneration.METHODS We used iPSCs,which retain their potential for cell proliferation.HSP20 overexpression effectively enhanced cell proliferation and pluripotency.Overexpression of HSP20 in iPSCs was characterized by immunocytochemistry staining and realtime polymerase chain reaction.We also used cell culture,cell counting,western blotting,and flow cytometry analyses to validate HSP20 overexpression and its mechanism.RESULTS This study demonstrated that overexpression of HSP20 can increase the pluripotency in iPSCs.Furthermore,by overexpressing HSP20 in iPSCs,we showed that HSP20 upregulated proliferation markers,induced pluripotent genes,and drove cell proliferation in a sirtuin 1(SIRT1)-dependent manner.These data have practical applications in the field of stem cell-based therapies where the mass expansion of cells is needed to generate large quantities of stem cell-derived cells for transplantation purposes.CONCLUSION We found that the overexpression of HSP20 enhanced the proliferation of iPSCs in a SIRT1-dependent manner.Herein,we established the distinct crosstalk between HSP20 and SIRT1 in regulating cell proliferation and pluripotency.Our study provides novel insights into the mechanisms controlling cell proliferation that can potentially be exploited to improve the expansion and pluripotency of human iPSCs for cell transplantation therapies.These results suggest that iPSCs overexpressing HSP20 exert regenerative and proliferative effects and may have the potential to improve clinical outcomes. | Mujib Ullah Nicole Pek Min Qian Gustavo Yannarelli Asma Akbar | 2021 | World Journal of Stem Cells2021，13，6： | 1 |
| 2 | Mesenchymalstromal cells mediate a switch to alternatively activatedmonocytes/macrophages after acute myocardial infarction显示文摘 | Dayan V Yannarelli G Billia F | 2011 | Basic Res Cardiol2011，106，6： | 1 |
| 3 | Heme oxygenase up-regulation in ultraviolet-B irradiation soybean plants involves reactive oxygen species显示文摘 | Yannarelli G G Noriega G O Batlle A Tomato M L | 2006 | Planta2006，224，5： | 1 |
| 4 | The effect of nitric oxide on heme oxygenase gene expression in soybean leaves显示文摘 | Guillermo O. Noriega Gustavo G. Yannarelli Karina B. Balestrasse Alcira Batlle Maria L. Tomaro | 2007 | Planta2007，，5： | 1 |
| 5 | Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species显示文摘 | Gustavo G. Yannarelli Guillermo O. Noriega Alcira Batlle Maria L. Tomaro | 2006 | Planta2006，，5： | 1 |
| 6 | Heme Oxygenase Up-regulation in Ultraviolet-B Irradiated Soybean Plants Involves Reactive Oxygen Speeies显示文摘 | Yannarelli G G Noriega G O Batlle A | 2006 | Planta2006，224，5： | 1 |
| 7 | Brief report:The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells显示文摘 | Yannarelli G Pacienza N Cuniberti L | 2013 | Stem Cells2013，31，： | 1 |
| 8 | Heme oxygenase up-regulation under salt stress protects nitrogen metabolism in nodules of soybean plants显示文摘 | Zilli C Z Balestrasse K B Yannarelli G G Polizio A H Santa-Cruz Tomaro M | 2008 | Environmental and Experimental Botany2008，64，： | 1 |
| 9 | Glutathione reductase activity and isoforms in leaves and roots of wheat plants subjected to cadmium tress 显示文摘 | Yannarelli G G Fernandez Alverze A J Santa - Cruz D M Tomaro M L | 2006 | Phytochemistry2006，11，： | 1 |
| 10 | Heme oxygenase and catalase gene expression in nodules and roots of soybean plants subjected to cadmium stress显示文摘 | BALESTRASSE K B YANNARELLI G G NORIEGA G O | | 0，，： | 1 |
| 11 | Angiotensin II regulates cardiac hypertrophy via oxidative stress but not antioxidant enzyme activities in experimental renovascular hypertension 显示文摘 | Polizio AH Balestrasse KB Yannarelli GG | 2008 | Hypertens Res2008，31，2： | 1 |
| 12 | Effect of UV-B radi- ation on the activity and isoforms of er~zymes with peroxidase ac- tivity in sunflower cotyledons显示文摘 | Yannarelli G G Gallego S M Tamaro M L | 2006 | Environmental and Experimental Botany2006，56，： | 1 |
| 13 | Mesenchymal stromal cells mediate a switch to alternatively activated monocytes/macrophages af-ter acute myocardial infarction 显示文摘 | Dayan V Yannarelli G Billia F ct al | 2011 | Basic Res Cardiol2011，106，6： | 1 |
| 14 | Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species 显示文摘 | YANNARELLI G G NORIEGA G O ALCIRA B | 2006 | Planta2006，224，5： | 1 |
| 15 | Mesenchymal stromal cells mediate a switch to ahematively activated monocytes/ macrophages after acute myocardial infarction显示文摘 | Dayan V Yannarelli G Billia F | 2011 | Basic Research in Cardiology2011，106，6： | 1 |
| 16 | Mesenchymal stromal cells mediate a switch to alternatively activated monoeytes/macrophages after acute myocardial infarction显示文摘 | Dayan V Yannarelli G Billia F | 2011 | Basic Res Cardio12011，106，6： | 1 |
| 17 | Mesenchymal stromal cells improve cardiac function and left ventricular remodeling in a heart transplantation model显示文摘 | MONTANARI S DAYAN V YANNARELLI G | 2015 | J Heart Lung Transplant2015，34，11： | 1 |
| 18 | Heme oxygenase up - regulation in ultraviolet - B irradiated soybean plants involves reactive oxygen species 显示文摘 | YANNARELLI G G NORIEGA G O BATLLE A | 2006 | Planta2006，224，： | 1 |
| 19 | Heme Oxygenase Up-Regulation in Ultraviolet-B Irradiated Soybean Plants Involves Reactive Oxygen Species 显示文摘 | YANNARELLI G G NORIEGA G O BATLLE A | 2006 | Planta2006，224，5： | 1 |
| 20 | Mesenchymal stromal cells mediate a switch to alternatively activated monocytes/macrophages after acute myocardial infarction显示文摘 | Dayan V Yannarelli G Billia F | 2011 | Basic Res Cardiol2011，106，6： | 1 |
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