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| 1 | Transcription factor ZmNAC126 plays an important role in transcriptional regulation of maize starch synthesis-related genes显示文摘Maize(Zea mays L.)is one of the most important food crops in the world,and starch is the main component of its endosperm.Transcriptional regulation plays a vital role in starch biosynthesis.However,it is not well understood in maize.We report the identification of the transcription factor ZmNAC126 and its role in regulation of starch synthesis in maize.Transcriptional expression of ZmNAC126 was higher in maize endosperm and kernels than in roots or stems.ZmNAC126 shared a similar expression pattern with starch synthesis genes during seed development,and its expression pattern was also consistent with the accumulation of starch.ZmNAC126 is a typical transcription factor with a transactivation domain between positions 201 and 227 of the amino acid sequence,is located in the nucleus,and binds to CACG repeats in vitro.Yeast one-hybrid assay revealed that ZmNAC126 bound the promoters of ZmGBSSI,ZmSSIIa,ZmSSIV,ZmISA1,and ZmISA2.Transient overexpression of ZmNAC126 in maize endosperm increased the activities of promoters pZmSh2,pZmBt2,pZmGBSSI,pZmSSIIIa,and pZmBT1 but inhibited the activities of pZmISA1 and pZmISA2.ZmNAC126 thus acts in starch synthesis by transcriptionally regulating targeted starch synthesis-related genes in maize kernels. | Qianlin Xiao Yayun Wang Hui Li Chunxia Zhang Bin Wei Yongbin Wang Huanhuan Huang Yangping Li Guowu Yu Hanmei Liu Junjie Zhang Yinghong Liu Yufeng Hu Yubi Huang | 2021 | The Crop Journal2021，9，1： | 5 |
| 2 | Extraction of rare earth ions from phosphate leach solution using emulsion liquid membrane in concentrated nitric acid medium显示文摘It is very significant to recover rare earths(REs) from wet-process phosphoric acid, in terms of extraction rate and selectivity, the current carrier di(2-ethlhexly) phosphate(D2EHPA) out there is still inferior.Based on this question, our team modified D2EHPA to synthesize new extractants. This paper presents a comprehensive study on the extraction of rare earth ions(RE^(3+)) from phosphate leach solution using emulsion liquid membrane(ELM) in concentrated nitric acid medium. The ELM system is made up of(RO)_2P(O)OPh-COOH as carrier, polyisocrotyl succinimide(T154) as surfactant, sulfonated kerosene as diluent, phosphoric acid(H_3PO_4) as stripping solution. Different chemical parameters such as type and concentration of carrier, surfactant, stripping solution, volume ratio of oil phase to internal phase, and volume ratio of emulsion ratio to external phase were analyzed. The extraction of RE^(3+) was evaluated by the yield of extraction. In addition, the demulsification process was also investigated. The proposed method of ELM using(RO)_2P(O)OPh-COOH as carrier can be expected to provide an efficient, simplify operation, and facilitated method for extracting RE^(3+). | Qianlin Chen Xin Ma Xin Zhang Yunqi Liu Ming Yu | 2018 | Journal of Rare Earths2018，36，11： | 5 |
| 3 | Efficiency of cotton bollworm (Helicoverpa armigera Hübner) control of different Bt cotton varieties in North China显示文摘Background: The cotton bollworm(Helicoverpa armigera) is one of cotton's most destructive insect pests in terms of yield and quality. Since 1997, China has grown commercially available transgenic Bacillus thuringiensis(Bt) cotton. We aimed to investigate the variation in resistance of transgenic Bt cotton varieties to cotton bollworm in North China.Methods: Populations of cotton bollworm were monitored from 2008 to 2015 in environments where Bt cotton was planted adjacent to other non-Bt crops. The study included 197 Bt cotton varieties planted in 42 counties/locations in three provinces(Hebei, Shandong and Henan) of North China, which were evaluated through field investigations,bioassays, and enzyme-linked immunosorbent assays(ELISA).Results: The average number of cotton bollworms never exceeded the action threshold(10 larvae per 100 cotton plants), however, their number reached 19.55 per 100 cotton plants in 2011. The ratios of damaged plants to total Bt cotton stem-tips, buds, and bolls was low except in 2010, when the ratios reached 1.82%, 2.09%, and 10.63%,respectively. The results of bioassay showed that the corrected mortality were higher at the second generation cotton bollworm stage than the third and fourth germination stages. Totally, Bt protein content declined sharply at the seedling stage from 2008 to 2015.Conclusions: This study indicated that almost all Bt cotton varieties were capable to effectively control the populations of cotton bollworm in North China. | Lü Limin LUO Junyu ZHANG Shuai YU Qianlin MA Ligang LIU Xiaofeng WANG Chunyi MA Xiaoyan MA Yan CUI Jinjie | 2018 | Journal of Cotton Research2018，1，1： | 1 |
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