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| 1 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 2 | Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins显示文摘Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including the ER for specific structural and functional needs.Peroxisomes are recently found to receive cholesterol from lysosomes through lysosomeperoxisome membrane contacts.However,whether and how cholesterol is conveyed from peroxisomes to the ER remain unknown.Here,by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes,we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(4,5)P2-containing liposomes to the ER in vitro,and that the presence of peroxisomes augments cholesterol transfer from lysosomes to the ER.Together,our study reveals a new cholesterol transport pathway along the lysosome-peroxisome-ER membrane contacts in the cell. | Jian Xiao Jie Luo Ao Hu Ting Xiao Meixin Li Zekai Kong Luyi Jiang Zimu Zhou Yacheng Liao Chang Xie Beibei Chu Honghua Miao Boliang Li Xiongjie Shi Bao-Liang Song | 2019 | Science China(Life Sciences)2019，62，9： | 8 |
| 3 | The non-canonical NF-κB pathway promotes NPC2 expression and regulates intracellular cholesterol trafficking显示文摘Niemann-Pick type C2(NPC2) is a lysosome luminal protein that functions in concert with NPC1 to mediate egress of lowdensity lipoprotein-derived cholesterol from lysosome. The nuclear factor kappa B subunit 2(NF-κB2) protein is a component of NF-κB transcription factor complex critically implicated in immune and inflammatory responses. Here, we report that NF-κB2 regulates intracellular cholesterol transport by controlling NPC2 expression. RNAi-mediated disruption of NF-κB2, as well as other signaling members of the non-canonical NF-κB pathway, caused intracellular cholesterol accumulation. Blockage of the non-canonical NF-κB pathway suppressed NPC2 expression, whereas Lymphotoxin β receptor(LTβR) activation or Baff receptor(BaffR) stimulation up-regulated the mRNA abundance and protein level of NPC2. Further, NF-κB2 activated NPC2 transcription through direct binding to its promoter region. We also observed cholesterol accumulation in NF-κB2-deficient zebrafish embryo and NF-κB2 mutant mice. Collectively, these data identify a regulatory role for the non-canonical NF-κB pathway in intracellular cholesterol trafficking and suggest a link between cholesterol transport and immune system. | Yacheng Liao Jian Wei Juqiong Wang Xiongjie Shi Jie Luo Bao-Liang Song | 2018 | Science China(Life Sciences)2018，61，10： | 4 |
| 4 | Generative urban design using shape grammar and block morphological analysis显示文摘The significant challenges that urban design faces when moving forward into this new digital era involve the increasingly massive and complex projects that must be analyzed and developed.The need for efficient analysis tools and rational design methods represent ongoing concerns,while practicable and maneuverable applications rather than experimental studies are scarce.This research implemented a framework of digital description and generative grammar of block form from the perspective of block morphological complexity.The implemented framework was tested in an urban design practice.First,this study adapted the hierarchical structure and access structure theory to analyze the spatial form of target blocks.The characteristics of blocks case samples in Nanjing are extracted as text descriptions.Second,the relevant composition patterns and parameters were employed as features for classificati on and were converted into procedural rules.With the top-down control by rules and bottom-up generation by shape grammar in CityEngine,the texture of the block can be generated as close to the actual block as possible.Furthermore,in a real urban design case located in Nanjing,this work applies the method to construct a three-dimensional scene quickly and accurately.After integrating design factors such as an environment,transportation,and vision and summarizing the intentions of blocks and buildings in corresponding functions and control indexes,the initial generation plan was built by applying the obtained characteristics and procedural rules in specific shape grammar.Finally,designers can adjust the result in detail by employing real-time calculation and interactive operation. | Xiao Wang Yacheng Song Peng Tang | 2020 | Frontiers of Architectural Research2020，9，4： | 4 |
| 5 | Multiple dimensions of urban design development from a practice perspective:A case study of an institute in Nanjing显示文摘The rapid urbanization process in China has promoted the active state of urban design practice.In the process of solving complex problems,the practical scope of urban design has gradually expanded.This paper took a design institution in Nanjing as a case study and sorted the trends in design project types and themes over the past 20 years,which presented a partial and diverse state of evolution in urban design practice.Concept,technology and management were extracted as the three main dimensions of cognitive urban design practice development.The relative positions and relationships of different types of urban design work were interpreted through mapping analysis of these three dimensions.This study provided a foundation for the practical orientation of urban design in China and a platform for communication a mong managers,urban design practitioners,scholars of related disciplines,and the public. | Dongqing Han Yacheng Song Enqi Wang Hua Liu Rong Fang | 2021 | Frontiers of Architectural Research2021，10，1： | 2 |
| 6 | On potential wells applications to sernilinear hyperbolic equation and parabolic equations显示文摘 | LIU Yacheng ZHAO Junsheng | 2006 | Nonlinear Analysis2006，，64： | 1 |
| 7 | On potential wells and vacuum isolating of solutions for semilinear wave equations显示文摘 | Liu Yacheng | 2003 | Jour Diff Eqs2003，192，1： | 1 |
| 8 | On potential wells and applications to semilinear hyperbolic equations and parabolic equations显示文摘 | Liu Yacheng Zhao Junsheng | 2006 | Nonlinear Analysis2006，64，： | 1 |
| 9 | On potential wells and applications to semilinear hyperbolic equations and parabolic equations显示文摘 | Liu Yacheng Zhao Junsheng | 2005 | Nonlinear Analysis2005，，12： | 1 |
| 10 | Fourth order wave equations with nonlinear strain and source terms显示文摘 | Yacheng Liu Runzhang Xu | 2006 | Journal of Mathematical Analysis and Applications2006，，1： | 1 |
| 11 | N pollution sources and denitrification in waterbodies in Taihu Lake region显示文摘 | Guangxi Xing Yacheng Cao Shulian Shi Guoqing Sun Lijuan Du Jianguo Zhu | 2001 | Science in China Series B: Chemistry2001，，3： | 1 |
| 12 | On potential wells and vacuum isolating of solutions for semilinear wave equations显示文摘 | Liu Yacheng | 2002 | Journal of Differential Equations2002，，1： | 1 |
| 13 | Genetic polymorphisms of 17 Y-STRs haplotypes in Chinese Han population residing in Shandong province of China 显示文摘 | Yan Jiangwei Tang Hui Liu Yacheng | 2007 | Legal Medicine2007，9，： | 1 |
| 14 | On potential wells and vacuum isolating of solutions for semilinear wave equations显示文摘 | Liu Yacheng | 2003 | J Differential Equations2003，192，： | 1 |
| 15 | Multidimensional viscoelasticity equations with nonlinear damping and source terms 显示文摘 | Liu Yacheng Zhao Junsheng | 2004 | Nonlinear Anal2004，56，： | 1 |
| 16 | On potential wells and vacuum isolating of solutions for semilinear wave equations 显示文摘 | Liu Yacheng | 2003 | J Differential Equations2003，192，： | 1 |
| 17 | On potential wells and applications to semilinear hyperbolic equations and parabolic equations 显示文摘 | Liu Yacheng Zhao Junsheng | 2006 | Nonlinear Anal2006，64，： | 1 |
| 18 | On potential wells and applications to semilinear hyperbolic equations and parabolic equations显示文摘 | Liu Yacheng Zhao Junsheng | 2006 | Nonlinear Analysis2006，64，： | 1 |
| 19 | Wave equations and reaction - diffusion equations with several nonlinear source terms of different sign显示文摘 | Liu Yacheng Xu Runzhang | 2007 | Discrete and Continuous Dynamical System - Series B2007，7，1： | 1 |
| 20 | Global existence, nonexistence and asymptotic behavior of solutions for the Cauchy problem of semilinear heat equations显示文摘 | Liu Yacheng Xu Runzhang Yu Tao | 2008 | Nonlinear Anal2008，68，11： | 1 |
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