| 2 | The miR167-OsARF12 module regulates rice grain filling and grain size downstream of miR159显示文摘Grain weight and quality are always determined by grain filling.Plant microRNAs have drawn attention as key targets for regulation of grain size and yield.However,the mechanisms that underlie grain size regulation remain largely unclear because of the complex networks that control this trait.Our earlier studies demonstrated that suppressed expression of miR167(STTM/MIM167)substantially increased grain weight.In a field test,the yield increased up to 12.90%-21.94% because of a significantly enhanced grain filling rate.Here,biochemical and genetic analyses revealed the regulatory effects of miR159 on miR167 expression.Further analysis indicated that OsARF12 is the major mediator by which miR167 regulates rice grain filling.Overexpression of OsARF12 produced grain weight and grain filling phenotypes resembling those of STTM/MIM167 plants.Upon in-depth analysis,we found that OsARF12 activates OsCDKF;2 expression by directly binding to the TGTCGG motif in its promoter region.Flow cytometry analysis of young panicles from OsARF12-overexpressing plants and examination of cell number in cdkf;2 mutants verified that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2.Moreover,RNA sequencing results suggested that the miR167-OsARF12 module is involved in the cell development process and hormone pathways.OsARF12-overexpressing plants and cdkf;2 mutants exhibited enhanced and reduced sensitivity to exogenous auxin and brassinosteroid(BR)treatment,confirming that targeting of OsCDKF;2 by OsARF12 mediates auxin and BR signaling.Our results reveal that the miR167-OsARF12 module works downstream of miR159 to regulate rice grain filling and grain size via OsCDKF;2 by controlling cell division and mediating auxin and BR signals. | Yafan Zhao Xiaofan Zhang Yuan Cheng Xiangxiang Du Sachin Teotia Chunbo Miao Huwei Sun Guoqiang Fan Guiliang Tang Hongwei Xue Quanzhi Zhao Ting Peng | 2023 | Plant Communications2023，4，5： | 1 |
| 3 | Experimental study of the effects of Ulinastatin on reducing MDA in free flaps显示文摘Free flap transplantation is the most important plastic surgery treatment.Although free flap is an important tissue repair tool for plastic surgery,it is easy to happen due to the anastomotic stenosis or thrombosis,as well as the inflammatory reaction caused by ischemia-reperfusion injury of the flap and the inflammation caused by these inflammatory reactions.Microcirculation contracture or occlusion will lead to partial or complete flap necrosis,thus affecting the repair effect.Numerous studies show that neutrophils are closely involved in the process of ischemic injury of free flaps.The purpose of this experiment is to investigate the use of ulinastatin to inhibit the inflammatory infiltration of free flaps to improve the survival of the flap. | Wei Lu Ruixia Qi Yifan Sun Yafan Sun Mingjuan Gu Xiang Gao Peng Yu Jing Sun | 2016 | Discussion of Clinical Cases2016，3，3： | 0 |
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