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| 1 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 2 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 3 | Renin-angiotensin-aldosterone system blockade for cardiovascular diseases:current status 显示文摘 | Ma TK Kam KK Yan BP | 2010 | Br J Pharmacol2010，160，6： | 1 |
| 4 | Durability of peginterferon alfa-2b treatment at 5 years in patients with hepatitis B e antigenpositive chronic hepatitis B显示文摘 | Wong VW Wong GL Yan KK | 2010 | Hepatology2010，51，： | 1 |
| 5 | Renin-angiotensin-aldosterone system blockade for cardiovascular diseases:current status显示文摘 | Ma TK Kam KK Yan BP | 2010 | Br P harmacol2010，160，6： | 1 |
| 6 | Clinicopathologic analysis of extracapsular extension in prostate cancer: Should the clinical target volume be expanded posterolaterally to account for microscopic extension? 显示文摘 | Chao KK Goldstein NS Yan D et at | 2006 | Int J Radiat Oncol Biol Phys2006，65，4： | 1 |
| 7 | Identification of anti-plasmin antibodies in the antiphospholipid syndrome that inhibit degradation of fibrin显示文摘 | Hwang KK Yan W | 2004 | J Immunol2004，172，9： | 1 |
| 8 | Durability of peginter- feron alfa -2b treatment at 5 years in patients with hepatitis B e antigen -positive chronic hepatitis B显示文摘 | WONG VW WONG GL YAN KK | 2010 | Hepatology2010，51，6： | 1 |
| 9 | Antitumor activity of IFIX, a novel interferon- inducible HIN-200 gene, in breast cancer 显示文摘 | Ding Y Wang L Su LK Frey JA Shao R Hunt KK Yan DH | 2004 | Oncogene2004，23，26： | 1 |
| 10 | A thrombin-cross-reac- tive anticardiolipin antibody binds to and inhibits the anti- coagulant function of activated protein C 显示文摘 | Hwang KK Yang CD Yan W | 2003 | Arthritis Rheum2003，48，6： | 1 |
| 11 | Novel valsartan-loaded solid dispersion with enhanced bioavailability and no crystalline changes 显示文摘 | Yan YD Sung JH Kim KK | 2012 | Int J Pharm2012，422，12： | 1 |
| 12 | Identification of anti-plasmin antibodies in the antiphospholipid syndrome that inhibitdegradation of fibrin显示文摘 | Yang CD Hwang KK Yan W | 2004 | J Immunol2004，172，9： | 1 |
| 13 | Identification of Antiplasmin antibodies in the antiphospholipid syndrome that inhibit degradation of fibrin 显示文摘 | HWANG KK YAN W | 2004 | J Immunol2004，172，9： | 1 |
| 14 | Novel valsartan-loaded solid dispersion with enhanced bioavailability and no crystalline changes 显示文摘 | Yan YD Sung JH Kim KK | 2012 | Int J Pharm2012，422，12： | 1 |
| 15 | Renin-angiotensin-aldosterone system blockade for cardiovascular diseases:current status显示文摘 | Ma TK Kam KK Yan BP | 2010 | Br J Pharmacol2010，160，6： | 1 |
| 16 | Durability of peginterferon alfa-2b treatment at 5 years in patients with hepatitis B e antigen-positive chronic hepatitis B显示文摘 | Wong VW Wong GL Yan KK | 2010 | Hepatology2010，51，： | 1 |
| 17 | Management of diaphragmatic rup- ture from blunt trauma显示文摘 | Tan KK Yan ZY Vijayan A | 2009 | Singapore Med J2009，50，4： | 1 |
| 18 | Induction of the Nrf2 - driven antioxi- dant response by tert - butylhydroquinone prevents ethanol - induced apoptosis in cranial neural crest cells显示文摘 | Yan D Dong J Sulik KK | 2010 | Biochemical Pharmacology2010，，80： | 1 |
| 19 | The effect of latanoprost and brimonidine on rabbit subconjunctival fibroblasts 显示文摘 | Lark KK Pasha AS Yan X | 1999 | J Glaucoma1999，8，1： | 1 |
| 20 | Social exchange processes in leisure and non-leisure settings:a review and exploratory investigation显示文摘 | Walker D Xie H Yan KK Maslov S | 1997 | Journal of Leisure Research1997，29，2： | 1 |
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