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| 1 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
| 2 | APE1 polymorphisms are associated with colorectal cancer susceptibility in Chinese Hans显示文摘AIM:To study the association between four base excision repair gene polymorphisms and colorectal cancer risk in a Chinese population.METHODS:Two hundred forty-seven colorectal cancer(CRC)patients and three hundred cancer-free controls were enrolled in this study.Four polymorphisms(OGG1Ser326Cys,APE1 Asp148Glu,-141T/G in the promoter region,and XRCC1 Arg399Gln)in components of the base excision repair pathway were determined in patient blood samples using polymerase chain reaction with confronting two-pair primers.The baseline information included age,gender,family history of cancer,and three behavioral factors[smoking status,alcohol consumption,and body mass index(BMI)].χ2tests were used to assess the Hardy-Weinberg equilibrium,the distributions of baseline characteristics,and the four gene polymorphisms between the cases and controls.Multivariate logistic regression analyses were conducted to analyze the correlations between the four polymorphisms and CRC risk,adjusted by the baseline characteristics.Likelihood ratio tests were performed to analyze the gene-behavior interactions of smoking status,alcohol consumption,and BMI on polymorphisms and CRC susceptibility.RESULTS:The APE1 148 Glu/Glu genotype was significantly associated with an increased risk of colorectal cancer(OR=2.411,95%CI:1.497-3.886,P<0.001relative to Asp/Asp genotype).There were no associations between OGG1,XRCC1,or APE1 promoter polymorphisms and CRC risk.A multivariate analysis including three behavioral factors showed that the APE1 148Glu/Glu genotype was associated with an increased risk for CRC among both smokers and non-smokers,non-drinkers and individuals with a BMI≥25 kg/m2(ORs=2.356,3.299,2.654,and 2.581,respectively).The XRCC1 399 Arg/Gln genotype was associated with a decreased risk of CRC among smokers and drinkers(OR=0.289,95%CI:0.152-0.548,P<0.001,and OR=0.327,95%CI:0.158-0.673,P<0.05,respectively).The APE1 promoter polymorphism-141 T/G genotype was associated with a reduced risk of colorectal cancer among subjects with a BMI<25 kg/m2(OR=0.214,95%CI:0.069-0.660,P<0.05 relative to T/T genotype).There were significant gene-behavior interactions between smoking status and XRCC1 Arg399Gln,as well as BMI and APE1-141T/G polymorphism(all P<0.05).CONCLUSION:APE1 Asp148Glu is associated with increased CRC risk and smoking alters the association between XRCC1 Arg399Gln and CRC risk in the Chinese Han population. | Shi-Heng Zhang Lin-Ang Wang Zheng Li Yu Peng Yan Ping Cun Nan Dai Yi Cheng He Xiao Yan-Li Xiong Dong Wang | 2014 | World Journal of Gastroenterology2014，20，26： | 8 |
| 3 | Mouse embryonic stem cells have increased capacity for replication fork restart driven by the specific Filia-Floped protein complex显示文摘Pluripotent 干细胞(PSC ) 在他们的快速的增长和作为结果的染色体不稳定性期间怀有组成的 DNA 复制应力在再生药妨碍他们的应用。理解复制的规章的机制在 PSC 强调反应因此是重要的。这里,我们报导胚胎的干细胞(转换字符) 是的那只老鼠比在解决复制应力的区分的房间优异。明确地,转换字符利用唯一的跳动 Filia 的蛋白质高效地支持阻止的复制的重启的复杂依赖者的机制分叉,因此维持 genomic 稳定性。转换字符特定的跳动 Filia 的建筑群在正常条件下面住在复制叉上。复制应力刺激他们的招募到阻止 Filia 的 151 残余是的叉和丝氨酸以一种 ATR 依赖的方式的 phosphorylated。这修正使跳动 Filia 的建筑群能充当功能的脚手架,它然后通过双机制支持拖延的叉重启:复制叉重启蛋白质, Blm,和刺激 ATR kinase 激活的两提高的招募。在 Blm 小径,脚手架招募 E3 ubiquitin ligase, Trim25,到阻止的复制叉,并且接着, Trim25 通过 ubiquitination 在阻止的复制叉拴住并且专注 Blm。在区分的房间,到复制叉和 ATR 发信号的激活的 Trim25-Blm 建筑群的招募由于转换字符特定的跳动 Filia 的支架的缺乏是更不柔韧的。因此,我们的学习表明转换字符利用另外、唯一的规章的层高效地支持阻止的叉重启并且维持 genomic 稳定性。 | Bo Zhao Weidao Zhang Yixian Cun Jingzheng Li Yan Liu Jing Gao Hongwen Zhu Hu Zhou Rugang Zhang Ping Zheng | 2018 | Cell Research2018，28，1： | 4 |
| 4 | Genetic variations of mTORC1 genes and risk of gastric cancer in an eastern chinese population显示文摘 | Jing He Meng‐Yun Wang Li‐Xin Qiu Mei‐Ling Zhu Ting‐Yan Shi Xiao‐Yan Zhou Meng‐Hong Sun Ya‐Jun Yang Jiu‐Cun Wang Li Jin Ya‐Nong Wang Jin Li Hong‐Ping Yu Qing‐Yi Wei | 2013 | Carcinog2013，，1： | 1 |
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