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| 1 | Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo显示文摘BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor metastasis.However,its role in hepatocellular carcinoma(HCC) cells remains unknown.METHODS:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical staining.Apoptosis was evaluated by TUNEL assay.Cell viability and apoptosis were determined by MTT assay and flow cytometry,respectively.Apoptosis-related proteins in SMMC-7721 and HepG2 cells were detected by Western blotting.RESULTS:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of 12-LOX.Baicalein inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent manner.Our in vivo study demonstrated that baicalein also reduced neoplastic nodules.Mechanistically,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of caspase-3.Furthermore,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of 12-LOX.CONCLUSIONS:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment. | Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，2： | 20 |
| 2 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 3 | Effects of salinity on methane gas hydrate system显示文摘Using an approximately analytical formation, we extend the steady state model of the pure methane hydrate system to include the salinity based on the dynamic model of the methane hydrate system. The top and bottom boundaries of the methane hydrate stability zone (MHSZ) and the actual methane hy-drate zone (MHZ), and the top of free gas occurrence are determined by using numerical methods and the new steady state model developed in this paper. Numerical results show that the MHZ thickness becomes thinner with increasing the salinity, and the stability is lowered and the base of the MHSZ is shifted toward the seafloor in the presence of salts. As a result, the thickness of actual hydrate occur-rence becomes thinner compared with that of the pure water case. On the other hand, since lower solubility reduces the amount of gas needed to form methane hydrate, the existence of salts in sea-water can actually promote methane gas hydrate formation in the hydrate stability zone. Numerical modeling also demonstrates that for the salt-water case the presence of methane within the field of methane hydrate stability is not sufficient to ensure the occurrence of gas hydrate, which can only form when the methane concentration dissolved in solution with salts exceeds the local methane solubility in salt water and if the methane flux exceeds a critical value corresponding to the rate of diffusive methane transport. In order to maintain gas hydrate or to form methane gas hydrate in marine sedi-ments, a persistent supplied methane probably from biogenic or thermogenic processes, is required to overcome losses due to diffusion and advection. | YANG DingHui1 & XU WenYue2 1 Department of Mathematical Sciences, Tsinghua University, Beijing 100084, China 2 School of Earth & Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA | 2007 | Science China Earth Sciences2007，50，11： | 5 |
| 4 | Insights into human CD34(+) hematopoietic stem/progenitor cells through a systematically proteomic survey coupled with transcriptome显示文摘 | Liu F Lu J Fan HH Wang ZQ Cui SJ Zhang GA Chi M Zhang X Yang PY Chen Z Han ZG | 2006 | 中国生物学文摘2006，20，5： | 2 |
| 5 | Systemic targeting in- hibitor of kappaB kinase inhibits melanoma tumor growth显示文摘 | Yang J Pan WH Clawson GA | 2007 | Cancer Res2007，67，7： | 1 |
| 6 | MMP-mediated disruption of claudin 5 in the blood-brain barrier of rat brain after cer ebral ischemia显示文摘 | Yang Y Rosenberg GA | 2011 | Methods Mol Biol2011，，762： | 1 |
| 7 | Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer显示文摘 | Zhang L Volinia S Bonome T Calin GA Greshock J Yang N | | 0，，： | 1 |
| 8 | t31ood-brain barrier breakdown in acute and chronic cerebrovascular disease显示文摘 | Yang Y Rosenberg GA | 2011 | Stroke2011，42，： | 1 |
| 9 | No relation between ACE-I/D polymorphism and high altitude pulmonary edema in the Han Chinese显示文摘Objectives To explore whether the angiotensin Ι-converting enzyme(ACE) I/D(insertion/deletion) polymorphism is associated with the susceptibility to high altitude pulmonary edema(HAPE) in the Han Chinese. Methods One hundred and forty-seven HAPE-p(HAPE patients) and 193 HAPE-r(HAPE resistants) were enrolled from the Yushu earthquake reconstruction workers in Qinghai province where the altitude is over 3 500 m above sea level. Blood samples were collected from each of the HAPE-p and HAPE-r groups. Information about physiological phenotypes was obtained via fieldwork investigation. The ACE-I/D polymorphism in HAPE-p and HAPE-r was detected by polymerase chain reaction(PCR). Results The SaO2 was significantly lower while HR was significant1y higher in HAPE-p group than those in HAPE-r group. The genotype frequencies of ACE-I/D for II, ID, DD in HAPE-r and HAPE-p groups were 0.430, 0.446, 0.124 and 0.435, 0.469, 0.095, respectively, the allelic frequencies of I and D were 0.650, 0.350 and 0.670, 0.330, respectively. The OR of ID, DD and D alleles relative to II for HAPE was 0.961(0.610~1.514), 1.322(0.634~2.758) and 1.080(0.783~1.489). There was no significant difference of the genotypic and the allelic frequencies in ACE-I/D polymorphism between HAPE-p and HAPE-r groups. Conclusions There is no relation between ACE-I/D polymorphism and HAPE in the Han Chinese. | Ying-zhong YANG Ya-ping WANG Wei GUAN Yang DU Qin GA Ri-li GE | 2013 | 中国应用生理学杂志2013，29，6： | 1 |
| 10 | Induction of the mitochondrial permeability transition causes release of the apoptogenic factor cytochrome C 显示文摘 | YANG JC CORTOPASSI GA | 1998 | Free Radic Biol Med1998，24，4： | 1 |
| 11 | Fuzzy Fault Diagnosis of a Diesel Engine Non-start显示文摘The diesel locomotive plays an important role in the field of transport,and the engine maintenance work is the prerequisite and guarantee for the locomotive normal working.In this paper,we first establish the fault tree model of locomotive engine 16V240ZJ on the basis of engine non-start as the top event.Then we combines the fuzzy mathematics theory and fault tree analysis method for failure diagnosis of 16V240ZJ engine's abnormal start-up.We obtained the fuzzy probability curve and top events probability confidence interval by analyzing the fuzzy fault tree qualitatively and quantitatively.It provides a fuzzy analysis basis for solving the problem of 16V240ZJ engine's abnormal start-up. | LIU Ke-ming YANG Wei-hong XU Guang-ming XU Wei-guo GA O Lei-fu | 2009 | International Journal of Plant Engineering and Management2009，14，3： | 1 |
| 12 | Diverse roles of matrix metalloproteinases and tissue inhibitors of metalloproteinases in neuroinflammation and cerebral ischemia显示文摘 | Candelario-Jalil E Yang Y Rosenberg GA | | 0，，03： | 1 |
| 13 | Vasogenic edema due to tight junction disruption by matrix metalloproteinases in cerebral ischemia显示文摘 | Rosenberg GA Yang Y | | 0，，05： | 1 |
| 14 | Vasogenic edema due to tight junction disruption by matrix metalloproteinases in cerebral ischemia显示文摘 | Rosenberg GA Yang Y | | 0，，： | 1 |
| 15 | Diverse roles of matrix metalloproteinases and tissue inhibitors of metanoproteinases in neuroinflammation and cerebral ischemia 显示文摘 | Candelario-Jalil E Yang Y Rosenberg GA | 2009 | Neuroscience2009，158，3： | 1 |
| 16 | Novel citric acid-based biodegradable elastomers for tissue engineering显示文摘 | Yang J Webb AR Ga A | 2004 | Adv Mater2004，16，8： | 1 |
| 17 | Systemic targeting inhibitor of kappaB kinase inhibits melanoma tumor growth显示文摘 | Yang J Pan WH Clawson GA | 2007 | Cancer Res2007，67，7： | 1 |
| 18 | MMP-mediated dis- ruption of claudin-5 in the blood-brain barrier of rat brain aftar cerebral ischemia显示文摘 | Yang Y Rosenberg GA | 2011 | Methods Mol Biol2011，762，： | 1 |
| 19 | Blood-brain barrier breakdown in acute and chronic cerebrovascular disease显示文摘 | Yang Y Rosenberg GA | 2011 | Stroke2011，42，： | 1 |
| 20 | Galectins: structure, function and therapeutic potential显示文摘 | Yang RY Rabinovich GA Liu FT | 2008 | Expert Rev Mol Med2008，10，： | 1 |
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